WANG Shang-de,WANG Xiao-chuan,LIU Jia-shen,et al.Pollen Morphology and Germination Characteristics of Peach Triploid Germplasms[J].Northern Horticulture,2015,39(12):80-84.[doi:10.11937/bfyy.201512022]
桃三倍体种质的花粉形态及萌发特性
- Title:
- Pollen Morphology and Germination Characteristics of Peach Triploid Germplasms
- 文章编号:
- 1001-0009（2015）12-0080-05
- 分类号:
- S 882.103.6
- 文献标志码:
- A
- 摘要:
- 以同起源的二倍体为对照，采用显微测量和离体培养方法，对3份桃三倍体种质的花粉形态及萌发特性进行了观察分析。结果表明：桃三倍体种质的花粉在大小、形态等方面存在巨大差异，花粉粒径大小分布无明显的规律，横径的众数占比约为20.0%~40.0%，远低于二倍体种质的66.0%~76.0%；三倍体种质的花粉萌发率低，仅4.1%~17.5%，而二倍体的萌发率高达60.2%~75.8%；二倍体花粉萌发花粉以体积为（67.1±7.1）×10.3 μm3的正常花粉为主，而三倍体种质‘10-15-23’,‘10-15-16’萌发最多的为体积分布在（95.3±7.1）×10.3 μm3的大花粉。
- Abstract:
- By microscopic measurement and in vitro germination，pollen morphology and germination characteristics of three triploid germplasms of peaches were observed and analysed，to contrast that of the same origin of diploid of peaches.The results showed that，for triploid，the size and shape of pollen varied between a large scale，no regular mode of the distribution of pollen grain sizes could be drawn，pollen minor diameter mode proportion of triploid ranges in 20.0%-40.0%,far lower than that of diploid，which was 66%-76%;the pollen germination rate of triploid was low，only 4.08%-17.52%，while that of diploid was up to 60.8%-75.8%.Thirdly，most of germinated pollens of diploid peaches were the ones with (67.1±7.1)×10.3 μm3 as their volume，While those of triploid ‘10-15-23’,‘10-15-16’ were larger pollens in volume （95.3±7.1)×10.3 μm3.
参考文献/References:
[1]Ramsey J，Schemske D W.Pathways，mechanisms and rates of polyploidy formation in flowering plants[J].Annu Rev Ecol Syst,1998,29:467-501.[2]Sarah P O.The evolutionary consequences of polyploidy[J].Cell,2007,131:452-462.[3]Husband B C.The role of triploid hybrids in the evolutionary dynamics of mixed ploidy populations[J].Biol J Linn Soc Lond,2004,82:537-546.[4]康向阳.林木多倍体育种研究进展[J].北京林业大学学报,2003,25(4):70-74.[5] Zeven A C.Polyploidy and domestriction:the origin and survival of polyploids in cytotype mixtures/in:polyploidy biological relevance[M].Plenum Press:New York,1980:385-407.[6]王同坤,张京政,齐永顺,等.我国果树多倍体育种研究进展[J].果树学报,2004,21(6):592-597.[7] Nico de Storme,Linda Z,Martin M,et al.Volume based pollen size analysis:an advanced method to assess somatic and gametophytic ploidy in flowering plants[J]. Plant Reprod.2013,DOI 10.1007/s00497/012-0209-0.[8]Jacob Y,Pierret V.Pollen size and ploidy level in the genus rosa[J].Acta Hortic,2000,508:289-292.[9]李绍华.桃树学[M].北京:中国农业出版社,2013.[10]Monet R，Bassi D.Classical genetics and breeding/the peach.botany,production and uses [M].London,CABI,2008:61-84.[11]Jansen B,Vonbothmer R.Pollen size in Hordeum L. correlation between size,ploidy level and breeding system[J].Sex Plant Reprod,1994,7:259-263. [12]Katsiotis A,Forsberg R A.Pollen grain size in four ploidy levels of genus Avena[J].Euphytica,1995,83:103-108.[13]Punyasingn K.Chromosome numbers in crosses of diploid triploid and tetraploid maize[J].Genetics,1947,32(6):541-554.[14]Wang L,Han D G,Gao C,et al.Paternity and ploidy segregation of progenies derived from tetraploid Malus xiaojinensis[J].Tree Genetics and Genomes,2012,8:1469-1476.[15]Diao W P,Bao S Y,Jiang B,et al.Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber[J].Biologia Plantarum, 2010,54(2):373-376.[16]Lazar M S,Tholath,de Storme N,et al.A screen for the identification of male meiotic mutants based on pollen size[J].Acta Horticulturae,2012,961:503-509.
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备注/Memo
第一作者简介：王尚德（1976-），男，硕士，副研究员，现主要从事果树学和细胞遗传学等研究工作。E-mail：shangdee@163.com.基金项目： 北京市农林科学院青年基金资助项目（QNJJ201306）；北京市农林科学院科技创新能力建设专项资助项目（KJCX20140108）；科技基础性工作专项资助项目(2013FY111700-2)。