HEWei-qiang,CHAOXin-dong,BAIJie.StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes[J].Northern Horticulture,2013,37(18):125-128.
香菇中多酚类物质提取工艺的优化研究
- Title:
- StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes
- 文章编号:
- 1001-0009(2013)18-0125-04
- 分类号:
- S646.1+2
- 文献标志码:
- A
- 摘要:
- 以香菇子实体为原料，采用Plackett-Burman设计对影响香菇多酚提取效率的8个因素进行筛选，并通过Box-Behnken设计及响应面分析法确定了影响多酚类提取因素的最佳条件。结果表明：影响香菇多酚提取的3个主要因素为：乙醇浓度、液料比和提取温度；最佳提取条件为：香菇过160目筛，以81%的乙醇作溶剂，液料比为34∶1mL/g，先超声处理15min，在85℃的水浴中提取45min，提取3次。根据最优试验条件对模型进行验证，得到香菇子实体的多酚提取量为186.50mg/100gFW，实际值与预测值的误差为3.24%。
- Abstract:
- TakingthefruitofLentinusedodesasmaterial,Plackett-BurmandesignwasusedtoevaluatetheinfluenceofrelatedeightfactorsoftotalphenolicfromLentinusedodes.AndtheBox-Behnkendesignandresponsesurfaceanalysiswereusedtodeterminetheoptimallevelsofthemainfactors.Theresultsshowedthatthreefactorsplayedtheimportantrolesinthemedium,includingethanolconcentration,temperatureandsolventsolidratio.Theoptimalconditionswereparticlesizelessthan160pore,15minforultrasonictime,34∶1mL/gforsolventsolidratio,81%ethanolforsolution,extractedat85℃for45min,extractingfor3times.Undertheoptimalconditions,theyieldoftotalphenolicreached186.50mg/100gFW,only3.24%differencesfromtheforecastbyRSM.
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备注/Memo
第一作者简介：贺伟强（1979-），男，河南郑州人，硕士，讲师，现主要从事天然活性成分的提取和分离技术等研究工作。
基金项目：嘉兴市科技计划资助项目（2012AY1058）。
收稿日期：2013-04-15