NING Jing,ZHAO He-xiang,DONG Ran,et al.Study on Selection and Propagation of Superior Individuals of Dictamnus dasycarps Turcz.[J].Northern Horticulture,2013,37(07):131-133.
白藓优良单株的选择及离体快繁技术研究
- Title:
- Study on Selection and Propagation of Superior Individuals of Dictamnus dasycarps Turcz.
- 文章编号:
- 1001-0009(2013)07-0131-03
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以野生白藓地下幼芽为外植体，进行优良单株的选择和组织培养研究，以期建立其快繁体系。结果表明：丛生芽诱导的最佳培养基为MS+6-BA 0.5 mg/L+NAA 1.0 mg/L，诱导率为90%；最佳增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.5 mg/L，增殖系数为4.6；最佳生根培养基为1/2MS+NAA 2.0 mg/L，生根率为70%。为提高试管苗的增殖率，其最佳继代周期为30 d。
- Abstract:
- Taking the plumule of Dictamnus dasycarps Turcz. underground as explants，the excellent individual plants were selected and the tissue culture of Dictamnus dasycarps Turcz.were established，in order to establish the propagation system.The results showed that the optimum medium for shoot induction was MS＋6-BA 0.5 mg/L＋NAA 1.0 mg/L，germination rate was 90％；the optimal proliferation medium was MS＋6-BA 0.5 mg/L＋NAA 0.5 mg/L，the multiplication coefficient was 4.6；the rooting medium was 1/2MS+NAA 2.0 mg/L，and its growth was the best and the rooting rate was 70％.In order to enhance the proliferation rate of test-tube plantlets，the best subculture period should be controlled in 30 d.
参考文献/References:
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备注/Memo
第一作者简介：宁静（1986-），女，在读硕士,现主要从事植物组织培养研究工作。