[1]陈爽,李金平,马会勤,等.不同植物生长调节剂对“金傲芬”无花果组培的影响[J].北方园艺,2013,37(03):109-111.
　CHEN Shuang,LI Jin-ping,MA Hui-qin,et al.Effect of Different Plant Growth Regulators on Tissue Culture of Ficus carica L.‘Jin Ao-fen’[J].Northern Horticulture,2013,37(03):109-111.
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不同植物生长调节剂对“金傲芬”无花果组培的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年03期 页码: 109-111 栏目: 出版日期: 2013-03-30
Title:
Effect of Different Plant Growth Regulators on Tissue Culture of Ficus carica L.‘Jin Ao-fen’
文章编号:
1001-0009（2013）03-0109-03
作者:
陈爽; 李金平; 马会勤; 张文
中国农业大学 农学与生物技术学院，果树逆境生理与分子生物学北京市重点实验室，北京 100193
Author(s):
CHEN Shuang; LI Jin-ping; MA Hui-qin; ZHANG Wen
College of Agriculture and Biotechnology，China Agricultural University，Key Laboratory of Beijing Municipality’s Stress Physiology and Molecular Biology for Fruit Trees，Beijing 100193
关键词:
无花果; “金傲芬”; 组织培养; 植物生长调节剂
Keywords:
Ficus carica L; ‘Jin Ao-fen’(A212); tissue culture; plant growth regulator
分类号:
S 482.8
文献标志码:
A
摘要:
以无花果“金傲芬”幼嫩带芽茎段为外植体，MS为基本培养基，采用常规组织培养方法，研究了不同植物生长调节剂对“金傲芬”无花果组织培养的影响。结果表明：2%的NaClO对外植体消毒6.5 min能起到较好的消毒效果，污染率为28%；适合“金傲芬”组培的初始培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂 8.0 g/L(pH 5.8);壮苗培养基为MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+GA 0.2 mg/L+蔗糖30 g/L+琼脂8.0 g/L（pH 5.8）；生根培养基为MS+IBA 1.0 mg/L+蔗糖 30 g/L+琼脂 8.0 g/L(pH 5.8)。
Abstract:
Taking young stems with buds of ‘Jin Ao-fen’ Ficus carica Linn as explants, on the basic MS medium，the effects of different plant growth regulators on shoot multiplication were studied.The results showed that the most appropriate time of disinfection of 2% NaClO was 6.5 min.The most appropriate medium of bud differentiation was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 8.0 g/L(pH 5.8);the most appropriate medium of making bud strong was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+GA 0.2 mg/L+sucrose 30 g/L+agar 8.0 g/L（pH 5.8）；the most appropriate rooting culture medium was MS+IBA 1.0 mg/L+sucrose 30 g/L+agar 8.0 g/L(pH 5.8).

参考文献/References:

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备注/Memo

第一作者简介：陈爽（1989-），女，在读硕士，研究方向为果树生理和栽培。E-mail:cspollyanna@yeah.net.

责任作者：张文（1955-），男，本科，副教授，硕士生导师，研究方向为果树生理和栽培。E-mail：zhangwen@cau.edu.cn.
基金项目：北京市都市农业学科群建设资助项目；北京市园林绿化局计划资助项目。

更新日期/Last Update: 2014-08-23