[1]刘鹏,董永义,陈永胜.蓖麻胚轴外植体离体再生研究[J].北方园艺,2013,37(03):107-109.
　LIU Peng,DONG Yong-yi,CHEN Yong-sheng.Study on Regeneration of Castor Bean in vitro Using Hypocotyl Explants[J].Northern Horticulture,2013,37(03):107-109.
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蓖麻胚轴外植体离体再生研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年03期 页码: 107-109 栏目: 出版日期: 2013-03-30
Title:
Study on Regeneration of Castor Bean in vitro Using Hypocotyl Explants
文章编号:
1001-0009(2013)03-0107-03
作者:
刘鹏1; 2; 董永义1; 陈永胜2; 3
1.内蒙古民族大学 农学院，内蒙古 通辽 028042；
2.内蒙古自治区高校蓖麻产业工程技术研究中心，内蒙古 通辽 028042；
3.内蒙古民族大学 生命科学学院，内蒙古 通辽 028042
Author(s):
LIU Peng1; 2; DONG Yong-yi1; CHEN Yong-sheng2; 3
1.College of Agriculture，Inner Mongolia University for Nationalities，Tongliao，Inner Mongolia 028042；
2.Engineering Research Center of Castor at Universities of Inner Mogolia Autonomons，Tongliao，Inner Mongolia 028042；
3.School of Life Science，Inner Mongolia University for Nationalities，Tongliao，Inner Mongolia 028042
关键词:
蓖麻; 胚轴; 离体再生
Keywords:
castor bean; hypocotyl; in vitro regeneration
分类号:
S 565.635.3
文献标志码:
A
摘要:
以蓖麻胚轴为外植体，研究了不同种类和浓度的细胞分裂素对胚轴再生不定芽的影响以及不同浓度的生长素对外植体根分化的影响。结果表明：从成熟蓖麻种子中剥离的胚轴接种在MS基本培养基并添加0.5 mg/L 6-BA或10.0 mg/L ZT中诱导不定芽的再生频率最高，分别为86.7%和68.9%。健壮的不定芽在MS+0.2 mg/L NAA的培养基中根分化率达26.7%。
Abstract:
Taking cotyledon explants of castor bean as materials，the effect of different species and concentrations of cytokinin on shoot induction and different concentrations of auxin on root induction were studied.The results showed that mature seed-derived hypocotyl explants produced adventitious shoots when placed on MS medium containing 0.5 mg/L 6-BA or 10.0 mg/L ZT.The rate of shoot regeneration was maximal (86.7% and 68.9% respectively).Shoots were transferred to root induction medium (MS+0.2 mg/L NAA)，which root regeneration frequency achieved 26.7%.

参考文献/References:

[1]Anjani K.Castor genetic resources：A primary gene pool for exploitation[J].Industrial Crops and Products,2012,35(1):1-14.

[2]Birch R G.Plant transformation：problems and strategies for practical application [J].Ann Rev Plant Physiol Plant Mol Biol 1997,48:297-326.

[3]Sujatha M，Sailaja M.Stable genetic transformation of castor (Ricinus communis?L.) via Agrobacterium tumefaciens-mediated gene transfer using embryo axes from mature seeds[J].Plant Cell Rep,2005,23:803-810.

[4]Malathi B，Ramesh S，Rao K V，et al.Agrobacterium-mediated genetic transformation and production of semilooper resistant transgenic castor (Ricinus communis L.)[J].Euphytica,2006,147:441-449.

[5]Ahn Y J，Chen G Q.In vitro?regeneration of castor (Ricinus communis?L.) using cotyledon explants[J].Hort Science,2008,43(1):215-219.

[6]Sujatha M，Reddy T P.Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis?L.)[J].Plant Cell Rep,1998(7):561-566.

[7]张利明,侯玲玲,李文彬,等.蓖麻组织培养和植株再生的研究[J].中国油料作物学报,2009,31(2):253-255.

[8]Alam I，Sharmin S A，Mondal S C，et al.?In vitro?micropropagation through cotyledonary node culture of castor bean (Ricinus communis?L.)[J].Australian Journal of Crop Science,2010,4(2):81-84.


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备注/Memo

第一作者简介：刘鹏（1973-），男，内蒙古赤峰人，博士，副教授，现主要从事分子生物学研究工作。E-mail:mindaliupeng@126.com.

责任作者：陈永胜（1971-），男，内蒙古通辽人，博士，教授，现主要从事分子生物学研究工作。E-mail:chenys2000@163.com.
基金项目：国家自然科学基金资助项目（31060194）；内蒙古自治区自然科学基金资助项目（2011MS0512）；内蒙古自治区高等学校科学研究资助项目（NJZY11201）。

更新日期/Last Update: 2014-08-23