[1]高苇,李宝聚,王万立,等.菌糠木霉发酵物对黄瓜枯萎病的防效研究[J].北方园艺,2013,37(16):140-143.
　GAOWei,LIBao-ju,WANGWan-li,et al.StudyonControlEffectof Trichoderma spp-spentMushroomSubstrateFermentationto CucumberFusarium Wilt[J].Northern Horticulture,2013,37(16):140-143.
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菌糠木霉发酵物对黄瓜枯萎病的防效研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年16期 页码: 140-143 栏目: 出版日期: 2013-08-30
Title:
StudyonControlEffectof Trichoderma spp-spentMushroomSubstrateFermentationto CucumberFusarium Wilt
文章编号:
1001-0009(2013)16-0140-04
作者:
高苇1; 2; 李宝聚2; 王万立1; 郝永娟1; 刘春艳1; 王勇1
1.天津市植物保护研究所，天津300381；
2.中国农业科学院蔬菜花卉研究所，北京100081
Author(s):
GAOWei 1; 2; LIBao-ju2; WANGWan-li1; HAOYong-juan1; LIUChun-yan1; WANGYong1
1.TianjinInstituteofPlantProtection，Tianjin300381；
2.InsitituteofVegetablesandFlowers，ChineseAcademyofAgriculturalSciences，Beijing100081
关键词:
菌糠; 木霉; 发酵物; 平板稀释法; 几丁质酶; β-1; 3-葡聚糖酶
Keywords:
mushroomsubstrate; Trichoderma spp; fermentation; platedilutionmethod; chitinase; β-1; 3-glucanase
分类号:
S436.421.1+3
文献标志码:
A
摘要:
以栽培平菇的棉籽壳为原料，研究了新型的土壤添加剂菌糠与生防木霉的发酵物对黄瓜枯萎病的防治效果。结果表明：菌糠木霉发酵物对黄瓜枯萎病的防治效果为63.58%，显著优于木霉的其它接种方式。采用平板稀释法检测根际木霉的数量，表明菌糠发酵物可以促进木霉在根际的定殖。通过酶活测定发现添加菌糠木霉发酵物的土壤中几丁质酶和β-1，3-葡聚糖酶的活性显著升高，分别在病原菌诱导后的第6天和第9天达到高峰，对病原真菌细胞壁的分解作用最强。
Abstract:
Takingcottonseedinmushroomcultivationasmaterial，thespentmushroomsubstratefermentationproductwasusedasanewtypeofsoiladditivesincucumbercultivationraw,itspreventioneffectoncucumber Fusariumwiltwasstudiedinthispaper.Theresultsshowedthatinpotexperiment，thecontroleffecttocucumberFusarium wiltwasupto63.58%insoiladdingspentmushroomsubstratefermentation，andwassignificantlybetterthanthatof Trichoderma sppotheraddingmethods.Trichodermasppaddingasspentmushroomsubstratefermentationwaseasiertocolonizeinthecucumberrhizospherthanotheraddingmethods.Basedontheenzymeactivityassay，itwasfoundthatchitinaseandβ-1,3-glucanaseactivitysignificantlyincreased，andrespectivelyreachedthepeakvalueat6and9daysundertheinductionof Fusariumoxysporum

参考文献/References:

[1]WeindlingR.Trichodermalignorumasaparasitcofothersoilfungi[J].Phytopathology,1932,22:837-845.
[2]JacksonAM,WhippsJM，LlynchJM.Nutritonalstudiesoffourfungiwithdiseasebiocontrolpotential[J].EnzymeMicrobiologytechnology,1991,13:456-461. 
[3]KredicsL.InfluenceofEnvironmentalparameterson Trichoderma Strainswithbiocontrolpotentia[J].Foodtechnologybiotechnology,2003,41(1):37-42.
[4]WhippsJM.Microbialinteractionsandbiocontrolintherhizosphere[J].JournalofExperimentBotany,2001,52:487-551. 
[5]HarmanGE，HayesCK,LoritoM,etal.Chitinolyticenzymesof Trichodermaharzianum：purificationofchitobiosidaseandendochitinase[J].Phytopathology,1993,83:313-318.
[6]TronSA，HarmanGE.DetectionandquantificationofN-Acetyl-β-D-glucosaminadase,chitoopsodase,andendochitinaseinsolutionsandongels[J].AnalBiochem,1993,208:74-79.
[7]ParhamJA，DengSP.Detection，quantificationandcharacterizationofβ-glucosaminidaseactivityinsoil[J].SoilBiologyBiochemistry,2000,32:1183-1190.
[8]郭润芳，刘晓光，高克祥,等．拮抗木霉菌在生物防治中的应用与研究进展[J]．中国生物防治，2002，18（4）：180-184.
[9]ZmoraNS，HadarY，ChenY.Pysico-chemicalpropertiesofcommercialcompostsvaryingintheirsourcematerialsandcountryoforigin[J].SoilBiology&Biochemistry，2007,10:101-114.

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备注/Memo

第一作者简介：高苇（1982-），女，辽宁大连人，博士，助理研究员，现主要从事植物病原真菌检测和防治的研究等工作。E-mail:gaowei5277@163.com.
 收稿日期：2013-04-15

更新日期/Last Update: 2014-09-12