HUMei-juan,ZHOUXing-chen,WANGLi,et al.ProducingEnzymeActivityandIdentificationAbout EndophyticActinomycetesIsolatedfromSophoraalopecuroides L.[J].Northern Horticulture,2013,37(16):98-102.
苦豆子内生放线菌产酶特性分析及活性菌株鉴定
- Title:
- ProducingEnzymeActivityandIdentificationAbout EndophyticActinomycetesIsolatedfromSophoraalopecuroides L.
- 文章编号:
- 1001-0009（2013）16-0098-05
- 关键词:
- 苦豆子; 内生放线菌; 产酶活性; 鉴定; 16SrRNA序列分析
- 分类号:
- S503.53
- 文献标志码:
- A
- 摘要:
- 以宁夏干旱荒漠区的126株苦豆子为试材，采用透明圈法对分离自其内生放线菌进行分泌胞外酶活性检测。结果表明：有71株内生放线菌具有酶活性，占总菌株数的56.3%。其中有3株菌株酶活性最为显著，菌株NDZKDS73、NDZKDS7具有的淀粉酶活性最高，透明圈半径达到24.62mm和19.12mm；菌株FJYKDS5具有的蛋白酶活性高，透明圈半径16.94mm；菌株FJYKDS11、BJGKDS2具有的纤维素酶活性最高，透明圈半径为24.72mm和22.40mm；菌株FJJKDS2具有的几丁质酶活性最高，透明圈半径17.76mm；菌株NDZKDS24、NDZKDS20对葡聚糖酶活性最高，透明圈半径为12.06mm和10.50mm。通过形态特征和16SrRNA基因序列同源性分析，有7株活性菌属链霉菌属(Streptomyces)，1株属拟诺卡氏菌属(Nocardiopsis)。
- Abstract:
- Taking126endophyticactinomycetesfromSophoraalopecuroidesL.inariddesertregionofNingxiaasmaterial,throughtransparentcirclemethod，studiedthefeatureofexcretingenzyme.Theresultsshowedthatthe71strainscouldproduceenzyme,accountingfor56.3%oftotalisolates,inwhich8strainswerethemostsignificantlyactive.Inthesestrains，NDZKDS73 andNDZKDS7strainsproducedthehighestactivityamylase，thetransparentcircleradiuswere24.62mmand19.12mm，respectively；FJYKDS5strainproducedthehighestactivityprotease，thetransparentcircleradiuswas16.94mm；FJYKDS11andBJGKDS2 strainsproducedthehighestactivitycellulase，transparentcircleradiuswere24.72mmand 22.40mm，respectively；FJJKDS2strainsproducedthehighestactivitychitinase，transparentcircleradiuswas17.76mm；NDZKDS24andNDZKDS20 strainsproducedhighestactivityglucanase，transparentcircleradiuswere12.06mmand 10.50mm，respectively.Bymorphologicalcharacteristicsand16SrRNAgenesequencesimilarityanalysis，7strainswereidentifiedas Streptomyces and1strainwere Nocardiopsis in8strains.
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备注/Memo
第一作者简介：胡美娟（1987-），女，宁夏银川人，硕士研究生，研究方向为生物防治及微生物资源利用。E-mail:598315218@qq.com.
责任作者：顾沛雯（1969-），女，宁夏银川人，博士，教授，现主要从事植物病理学的教学与科研工作。E-mail：gupeiwen2005@yahoo.com.cn.
基金项目：国家自然科学基金资助项目（30960228）；宁夏科技支撑计划资助项目（2007BAD57B02）；宁夏自然科学基金资助项目（nz1002）。
收稿日期：2013-04-10