QI He-xing,HU Mei-juan,WANG Li,et al.Screening and Identification of Antagonistic Endophytic Actinomycetes Isolated from Sophora alopecuroides L.[J].Northern Horticulture,2014,38(08):86-91.
苦豆子内生拮抗放线菌的筛选及活性菌株鉴定
- Title:
- Screening and Identification of Antagonistic Endophytic Actinomycetes Isolated from Sophora alopecuroides L.
- 文章编号:
- 1001-0009（2014）08-0086-06
- 关键词:
- 苦豆子; 内生放线菌; 抑菌活性; 鉴定; 16S rRNA序列分析
- Keywords:
- Sophora alopecuroides L.; endophytic actinomycetes; antimicrobial activity; identification; 16S rRNA sequence analysis
- 分类号:
- S 476
- 文献标志码:
- A
- 摘要:
- 以6种植物病原真菌为靶标菌，采用皿内对峙培养法，对126株苦豆子内生放线菌进行了抗菌活性筛选。结果表明：15株菌具有较高的抑菌活性，占总菌株数11.9%，其中菌株YWZKDS4和NDZKDS65对稻瘟病病菌和黄瓜枯萎病菌的抑菌带宽度最大，分别为15.12 mm和18.54 mm；菌株NDZKDS22具有广谱抗菌活性，它们的抑菌带宽度均大于10 mm以上；根据形态特征和16S rRNA基因序列同源性分析，3株活性菌株YWZKDS4、NDZKDS22、NDZKDS65分别与Streptomyces scabiei、Streptomyces albospinus和Streptomyces capillispiralis亲缘关系最近，序列相似性分别为99.1%、96.4%、98.8%。试验结果表明，苦豆子内生放线菌对6种植物病原真菌具有较好的抑菌活性。
- Abstract:
- Taking six kinds of plant pathogenic fungi as target bacteria，culture dishes method was adopted,antibacterial activity of 126 endophytic actionmycetes strains isolated from .alopecuroides L.were tested.The results showed that 15 strains had strong inhibiting effect on target pathogens，accounting for 11.9% of total isolates.Of them，inhibition zones of YWZKDS4 against Pyricularia oryzae and NDZKDS65against Fusarium oxysporum reached 15.12 mm and 18.54 mm respectively,NDZKDS22 had broad-spectrum antifungal activity，their inhibition zones against 5 species of target pathogens were more than 10 mm.The closest genetic relationships of YWZKDS4 with Streptomyces scabiei,NDZKDS22with S.albospinus，and NDZKDS65with S.capillispiralis were found respectively by morphological characteristics and 16S rRNA sequence similarity analysis.Sequence similarity were 99.1%，96.4%，98.8%.According to the experiment，the antimicrobial activity of endophytic actinomycetes from ?S.alopecuroides L.against 6 species of target plant pathogens was better.
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备注/Memo
第一作者简介：祁鹤兴（1990-）,女,青海西宁人,硕士研究生,研究方向为生物防治及微生物资源利用。E-mail:390495559@qq.com.