[1]于安民,杨锦芬,詹若挺,等.贮存条件对阳春砂花粉活力的影响[J].北方园艺,2013,37(10):147-150.
　YU An-min,YANG Jin-fen,ZHAN Ruo-ting,et al.Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum Lour.[J].Northern Horticulture,2013,37(10):147-150.
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贮存条件对阳春砂花粉活力的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年10期 页码: 147-150 栏目: 出版日期: 2013-06-15
Title:
Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum  Lour.
文章编号:
1001-0009(2013)10-0147-04
作者:
于安民1; 2; 杨锦芬1; 2; 詹若挺1; 2; 陈蔚文1; 2
1.广州中医药大学 中药资源科学与工程研究中心，广东 广州 510006；
2.岭南中药资源教育部重点实验室(广州中医药大学)，广东 广州 510006
Author(s):
YU An-min1; 2; YANG Jin-fen1; 2; ZHAN Ruo-ting1; 2; CHEN Wei-wen1; 2
1.Research Center of Chinese Herbal Resource Science and Engineering，Guangzhou University of Traditional Chinese Medicine，Guangzhou,Guangdong 510006；
2.Key Laboratory of Chinese Medicine Resource from Lingnan(Guangzhou University of Chinese Medicine),Ministry of Education，Guangzhou,Guangdong 510006
关键词:
阳春砂; 花粉活力; 贮存条件; 离体萌发法; MTT染色法
Keywords:
A.villosum; pollen viability; preservation condition; pollen germination in vitro; MTT staining method
分类号:
S 567.23+9
文献标志码:
A
摘要:
以种植于广州中医药大学的阳春砂为试材，将收集的花粉贮存在不同的温度和湿度条件下，分别在贮存0、3、6、9、12、24、48 h后，用离体花粉萌发法和噻唑蓝(MTT)染色法测定花粉活力，以期探讨不同贮存条件对阳春砂花粉活力的影响。结果表明：适当的高温和高湿度条件能延长阳春砂花粉的贮存寿命。离体花粉萌发法比MTT染色法能更真实的反映阳春砂花粉活力；27℃+高湿度的条件最适合阳春砂离体花粉的短期(≤9 h)贮存，这与阳春砂喜高温高湿的生长习性相符。
Abstract:
Taking Amomum villosum Lour. that cultivated in Guangzhou University of Chinese Medicine as material，the effect of different preservation conditions on the pollen viability of Amomum villosum Lour. were studied.The collected pollen were kept in different conditions for 0，3，6，9，12，24 and 48 hours respectively，then the pollen viability were determined using MTT staining method and in vitro germination method.The results showed the moderately high temperature and high humidity prolonged the life of pollen from A.villosum. In vitro germination method was appropriate to reveal the pollen viability.The optimum shortterm storage(≤9 h)condition for pollen of A.villosum was 27℃ high humidity，which was consistent with the growth habit of high temperature and humidity.

参考文献/References:

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备注/Memo

第一作者简介：于安民(1987-)，女，在读硕士,研究方向为中药生物技术。E-mail:sdynyam@163.com.
责任作者：杨锦芬(1978-)，女，博士，副研究员，现主要从事中药生物技术研究等工作。E-mail:yangjf@gzucm.edu.cn.
基金项目：南药可持续利用研究与综合开发利用技术平台资助项目(粤财教[2010]358)；广东省自然科学基金面上资助项目(S2011010003717)。
收稿日期：2013-01-16

更新日期/Last Update: 2014-08-15