MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(10):107-110.
分子标记辅助选择辣椒抗疫病新种质研究
- Title:
- Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight
- 文章编号:
- 1001-0009(2013)10-0107-04
- Keywords:
- hot pepper; phytophthora blight; molecular markers; breeding
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 以野生辣椒‘12-16’和甜椒‘三道筋’、‘12-1’、‘12=19’为试材，选用与辣椒疫病抗性相关的4对引物，对4份辣椒材料的疫病抗性进行了快速鉴定。结果表明：4份辣椒材料中，‘12-16’中能够扩增出抗病特异性条带，推断其为抗病材料；其它3份材料均未扩增出抗病特异性条带，表明其为感病材料；供试的4对引物中仅有1对引物的稳定性和重复性较高。
- Abstract:
- Taking pepper ‘12-16’，‘Sandaojin’，‘12-1’，‘12-19’ as materials，their resistance to phytophthora blight were rapidly identified by using 4 molecular markers related to resistance genes and field surveys.The results showed that phytophthora blight resistance specificity band could be amplified from hot pepper materials，namely‘12-16’.The other 3 hot pepper materials，‘Sandaojin’，’12-1’，and ’12-19’，with no specificity band found，were regarded as susceptible materials.Only one pair of primers of the 4 pairs of primers reported was stable repeated.
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备注/Memo
第一作者简介：马寿宾(1987-)，男，在读硕士，研究方向为蔬菜育种与生物技术。E-mail:mashoubin1987@163.com.
责任作者：刘维信(1965-)，男，博士，副教授，硕士生导师，研究方向为蔬菜遗传育种与生物技术。E-mail:liuweixin2006@163.com.
基金项目：2010～2012年山东省现代农业产业技术体系蔬菜创新团队建设资助项目。
收稿日期：2013-01-18