SUN Xiao-mei,WANG Hui-cong,ZHOU Wen-qiang,et al.Preliminary Study on Embryo Culture in vitro of Paeonia lactiflora Pall.[J].Northern Horticulture,2013,37(10):97-99.
芍药胚离体培养的初步研究
- Title:
- Preliminary Study on Embryo Culture in vitro of Paeonia lactiflora Pall.
- 文章编号:
- 1001-0009(2013)10-0097-03
- Keywords:
- Paeonia lactiflora Pall.; embryo; in vitro culture
- 分类号:
- S 682.1+2
- 文献标志码:
- A
- 摘要:
- 以芍药去皮种子和胚为外植体，研究了不同培养基对胚萌发率和生长状况的影响。结果表明：在诱导种子萌发过程中，3种基本培养基中以MS的效果最好，且在MS+6-BA 2.0 mg/L+NAA 0.2 mg/L培养基上萌发率最高，达71.4%;离体胚培养中，单独添加2.0 mg/L 6-BA时，胚生长受到抑制；MS+6-BA 1.0 mg/L+NAA 0.4 mg/L能促进愈伤组织形成；适宜浓度的GA-3能够打破上胚轴休眠，MS+6-BA 1.0 mg/L+GA30.5 mg/L为胚诱导真叶抽生的最佳培养基。
- Abstract:
- Paeonia lactiflora Pall.peeled seeds and embryos were used as explants to study the effects of different mediums on the germination and growth of Paeonia lactiflora Pall..The results showed that MS medium was the best for inducing the germination of seeds among 3 medias and seeds had the highest germination rate (71.4%) on MS+6-BA 2.0 mg/L+NAA 0.2 mg/L.In vitro embryo culture，adding 6-BA solely at 2.0 mg/L inhibited the growth of embryo；MS+6-BA 1.0 mg/L+NAA 0.4 mg/L promoted the formation of callus；GA3 at suitable concentration could break the dormancy of epicotyl and the optional medium for inducing leaves to throw out from embryo was MS+6-BA 1.0 mg/L+GA30.5 mg/L.
参考文献/References:
[1]胡映泉,冯海华,时宝凌.芍药不定芽的诱导技术[J].山西林业科技,2003(增刊):23-33.
[2]张庆瑞,杨秋生,李永华.不同植物生长调节物质对芍药离体培养的影响[J].河南农业大学学报,2007,41(1):25-28.
[3]王吉凤.芍药组织培养研究[D].北京:北京林业大学,2009.
[4]于晓南,吴红娟,潘瞳.4个品种芍药愈伤组织的诱导及分化[J].湖南农业大学学报(自然科学版),2001,37(2):166-171.
[5]王瑶,岳桦.芍药组培中抗外植体褐化相关问题的研究[J].湖北农业科学,2009,48(4):783-786.
[6]郑相穆,周阮宝,谷丽萍,等.凤丹种子的休眠和萌发特性[J].植物生理学通讯,1995,31(4):260-262.
[7]赵明,张松荣,何小弟,等.芍药愈伤组织诱导及遗传转化技术的初步研究[J].林业实用技术,2009(1):10-12. [8]于恒秀,周美艳,王淼,等.利用二次正交旋转组合设计优化芍药胚苗快繁的培养基[J].扬州大学学报(农业与生命科学版),2008,29(4):84-89.
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备注/Memo
第一作者简介：孙晓梅(1970-)，女，博士，教授，研究方向为园林植物遗传育种。
基金项目：国家自然科学基金资助项目(31240028)。
收稿日期：2012-01-14