SUN Jing-xian,GAO Yun-tao,ZHANG Ji,et al.Comparison of Pteris vittata L.Propagation Between Tissue Culture and Natural Sowing[J].Northern Horticulture,2013,37(05):91-93.
蜈蚣草组织培养与自然播种繁殖的比较研究
- Title:
- Comparison of Pteris vittata L.Propagation Between Tissue Culture and Natural Sowing
- 文章编号:
- 1001-0009(2013)05-0091-03
- Keywords:
- Pteris vittata L.; spore; tissue culture; natural sowing; compare
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以蜈蚣草（Pteris vittata L.）孢子为外植体，研究了蜈蚣草无性系快繁体系技术，比较分析了蜈蚣草组织培养和自然播种繁殖的效果。结果表明：蜈蚣草孢子萌发条件并不苛刻，无论在培养基中，还是普通基质中都能萌发，且萌发率都在90%以上，分化率也较高。故而，可以创造适合孢子萌发的人工条件，通过自然播种,低成本地获得大量蜈蚣草种苗；同时可以通过组织培养快速繁殖优良的蜈蚣草株系，并在相对干净的背景下研究微生物与蜈蚣草对砷吸收的相互作用。
- Abstract:
- Taking spore of Pteris vittata L.as material，its rapid propagation with clone system were studied,and the propagation of tissue culture and natural sowing of Pteris vittata L.were compared.The results showed that the sprouting condition of its spores was not rigorous.They could sprout both in medium and normal matrix.The germination rate could reach more than 90%，and the differentiation rate was also high.Therefore，low-cost seedlings could be obtained cheaply through natural sowing with spores under artificial conditions.In the other hand，the screened strains of Pteris vittata L.plantlet could be reproduced by tissue culture.Consequently the interaction between microbe and Pteris vittata L.on the absorption of arsenic could be studied under a relatively clean background.
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备注/Memo
第一作者简介：孙静贤（1978-），女，硕士，讲师，研究方向为生物技术。E-mail:kmsjx@163.com.
基金项目：云南省本科教学实验生物技术示范中心资助项目（0205-2001015209）。
收稿日期：2012-11-07