ZHU Mei-xia,HAN Gai-ying,WANG Lan-ming,et al.Molecular Cloning and Sequence Analysis of afp1 Gene in Momordica charantiap[J].Northern Horticulture,2012,36(24):120-123.
苦瓜afp1基因的克隆与序列分析
- Title:
- Molecular Cloning and Sequence Analysis of afp1 Gene in Momordica charantiap
- 文章编号:
- 1001-0009（2012）24-0120-04
- Keywords:
- Momordica charantiap; afp1; RT-PCR; clone; sequene analysis
- 分类号:
- Q 943.2
- 文献标志码:
- A
- 摘要:
- 通过异硫氰酸胍法提取苦瓜总RNA，采用一步RT-PCR方法获得394 bp苦瓜afp1基因序列。应用DNA star软件进行序列分析，表明这个片段包含了基因完整的编码。Blast分析表明,它与荠菜的防卫基因有很高的同源性，序列一致率为87%。该试验结果将为分子植物抗病育种提供基因材料。
- Abstract:
- Total RNA of Momordica charantiap was extracted using guanidine isothiocyantehod method.afp1 gene（394 bp）of Momordica charantiap was obtained by a step of RT-PCR method.afp1sequence was analyzed using DNA star software，the results showed afp1 sequence contained complete open reading frame.Identity percent of it with defensin gene of Brassica juncea was 87%.The results of the project would provide experimental evidence for molecular breeding of plants disease resistance.
参考文献/References:
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备注/Memo
第一作者简介：朱美霞（1970-），女，河北武安人，硕士，副教授，现主要从事植物分子育种和分子生物学等方面的教学和科研工作。E-mail:zmx0412@163.com.