SUN Fang,NIU Tian,LI Cheng-xiu,et al.Establishment of Rapid Propagation System of Hybrid Embryo Between Cymbidiumkanran Makino and Cymbidium faberi Rolfe[J].Northern Horticulture,2012,36(24):124-128.
寒兰与蕙兰杂交种胚快繁体系的建立
- Title:
- Establishment of Rapid Propagation System of Hybrid Embryo Between Cymbidiumkanran Makino and Cymbidium faberi Rolfe
- 文章编号:
- 1001-0009（2012）24-0124-03
- Keywords:
- hybrid Cymbidium; rhizome; proliferation times; differentiation rate
- 分类号:
- S 682.31
- 文献标志码:
- A
- 摘要:
- 为加快杂交兰快繁体系的建立，以蕙兰‘新上海’（♂）与寒兰‘寒香梅’（♀）杂交种胚获得的根状茎为外植体，采用不同浓度NAA和6-BA配比组合，研究其对杂交兰增殖与分化的影响。结果表明：杂交根状茎在MS+1.0 mg/L NAA+0.1 mg/L 6-BA的基本培养基中增殖效果最好，增值倍数达到3.08。在培养基MS+0.1 mg/L NAA+5 mg/L 6-BA的培养基上分化产生的不定芽数量最多。1/2MS+1.0 mg/L NAA+0.2%AC培养条件下，不定根得到很好诱导。激素配比可以很好的加快杂交兰的快速繁殖。
- Abstract:
- In order to speed up the building of the rapid propagation system for hybrid cymbidium，taking rhizome of ‘Xinshanghai’(♂)בHanxiangmei’(♀) as explants,the effects of different concentrations of 6-BA and NAA combinations on rapid propagation and differention were studied.The results showed that MS+1.0 mg/L NAA+0.1 mg/L 6-BA was the highest propagation coefficient and it could reach 3.08.MS+0.1 mg/L NAA+5 mg/L 6-BA was the optimum medium for adventitious bud regeneration.1/2MS+1.0 mg/L NAA+0.2%AC could be good for adventitious roots’ induction.The use of hormone would speed up the rapid propagation of hybrid Cymbidium.
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备注/Memo
第一作者简介：孙芳（1987-），女，山东潍坊人，在读硕士，研究方向为园林植物遗传育种。E-mail:yufang12221987@126.com.