MA Li,WANG Yu-hai,MING Dong-feng.Analysis of Genetic Relationship for Punica granatum L.Germplasm Based on Peroxidase Isozyme[J].Northern Horticulture,2012,36(19):124-127.
石榴种质资源过氧化物酶同工酶的亲缘关系分析
- Title:
- Analysis of Genetic Relationship for Punica granatum L.Germplasm Based on Peroxidase Isozyme
- 文章编号:
- 1001-0009（2012）19-0124-04
- Keywords:
- Punica granatum L.(pomegranates); germplasm; POD isozyme; cluster analysis; genetic relationship
- 分类号:
- S 665.4
- 文献标志码:
- A
- 摘要:
- 采用聚丙烯酰胺凝胶电泳方法对19份石榴品种的过氧化物酶（POD）同工酶酶谱进行分析，并利用系统聚类的方法，研究了19个品种间的亲缘关系。结果表明：19个品种的POD同工酶酶谱多态性较高，不同品种的酶带数目、相对迁移率及酶活性均有差异；19个石榴品种的POD同工酶酶谱共呈现12条酶带，其中1条酶带为所有种质共有的酶带；Rf为0.216的谱带为大青皮酸品种的特征谱带，Rf为0.113的酶带为大马牙甜石榴的特征谱带。POD同工酶聚类分析结果在阈值0.60处将19个品种分为5类，类群间遗传差异较大。
- Abstract:
- The peroxidase (POD) isozyme of 19 pomegranates germplasm cultivars were determined by polyarcylamide gel electrophoresis (PAGE) and the genetic relationship were studied with clustering analysis method.The results showed that a better polymorphism was observed in POD enzyme.The number，the activity and Rf of POD enzyme were difference among 19 pomegranates.12 enzyme bands were detected and there was a common locus for total experiment material in the zymogram patterns；the two specific locuses that Rf was 0.216 and 0.113 were obtained for ‘Daqingpisuan’ cultivar and ‘Damayatian’ cultivar respectively.Furthemore，the clustering analysis indicated 19 pomegranates were divided into five groups based on 0.60 of similarity coefficient，which indicated that the genetic difference between groups was significant.
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备注/Memo
第一作者简介：马丽（1980-），女，博士，副教授,现主要从事石榴分子生物学遗传育种研究工作。E-mail:mary1976816@163.com.