[1]韦坤华,李林轩,缪剑华,等.姜种质资源离体保存技术研究[J].北方园艺,2013,37(08):112-116.
　WEI Kun-hua,LI Lin-xuan,MIAO Jian-hua,et al.In vitro Conservation Technique of Germplasm in Zingiber officinale Rosc.[J].Northern Horticulture,2013,37(08):112-116.

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姜种质资源离体保存技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷期数: 2013年08期页码: 112-116 栏目: 出版日期: 2013-04-30

Title:: In vitro Conservation Technique of Germplasm in Zingiber officinale Rosc.

文章编号:: 1001-0009（2013）08-0112-05

作者:: 韦坤华¹; 2; 李林轩¹; 缪剑华¹; 高山林²; 1.广西壮族自治区药用植物园，广西药用资源保护与遗传改良重点实验室，广西南宁 530023；
2.中国药科大学遗传育种教研室，江苏南京 211198

Author(s):: WEI Kun-hua¹; 2; LI Lin-xuan¹; MIAO Jian-hua¹; GAO Shan-lin²; 1.Guangxi Botanical Garden of Medicinal Plants,Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning，Guangxi 530023；
2.Department of Genetics and Breeding，China Pharmaceutical University，Nanjing，Jiangsu 211198

关键词:: 姜; 种质资源; 离体保存; 生长恢复; 遗传稳定性

Keywords:: Zingiber officinale Rosc.; germplasm resource; in vitro conservation; growth recovery; genetic stability

分类号:: S 632.502

文献标志码:: A

摘要:: 采用正交实验设计，研究了生长调节素、渗透压、温度等因素对姜种质资源离体保存的影响，以期建立姜种质资源离体保存技术体系，并通过生长恢复与遗传稳定性考察对保存材料进行初步评价。结果表明：生长调节剂、渗透压、温度等因素对姜种质资源的离体保存均有显著影响，最终确定姜离体保存的最佳保存方法为：MS+蔗糖60 g/L+琼脂4.0 g/L+甘露醇10 g/L+矮壮素(CCC)2.0 mg/L+多效唑(PP₃₃₃)2.0 mg/L+脱落酸(ABA)2.0 mg/L，培养条件为光照时间14～16 h/d，光照强度1 500 lx，温度20℃，在此条件下保存300 d，存活率在50%以上，保存材料生长恢复情况良好，染色体保持稳定。

Abstract:: To establish the optimal in vitro conservation technology system of Zingiber officinale Rosc.germplasm，the effects of phytohormone，osmotic pressure and temperature on it were tested，and the growth recovery and genetic stability were evaluated after 300 days of in vitro conservation.The results showed that the best conservation conditionwas MS basal medium containing 6 g/L sucrose，4.0 g/L agar powder and 10 g/L mannite supplemented with 2.0 mg/L CCC，2.0 mg/L PP₃₃₃ and 2.0 mg/L ABA，in an illuminated chambr under 14~16 h photoperiod of 1 500 lx light intensity at 20℃，more than 50% conservation material survived after 300 days，most of them could grew well on propagation medium，and the chromosome number maintained stably.

参考文献/References:

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[8]Wither L A.Technology and prospects of cryopreservation of germplasm[A].In Holden J H W,J T Williamsed.Crop Genetic Resources:Conservation and Evaluation”.George Allen and Unwib (Pubilsher) Ltd,1984:138-157.

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备注/Memo

第一作者简介：韦坤华（1983-），女，博士，助理研究员，现主要从事中药生物技术和资源保护等研究工作。E-mail:divinekh@163.com.

责任作者：高山林（1946-），男，教授，博士生导师，现主要从事中药生物技术研究工作。E-mail：shlingao@163.com.

更新日期/Last Update: 2014-08-25