ZENG Ying-ping,ZHU Qian-kun,WANG Wan-jun.The Coating on Artificial Seeds of Dendrobium candidum Wall ex Lindl.[J].Northern Horticulture,2012,36(17):155-158.
铁皮石斛人工种子包衣技术研究
- Title:
- The Coating on Artificial Seeds of Dendrobium candidum Wall ex Lindl.
- 文章编号:
- 1001-0009(2012)17-0155-04
- 分类号:
- S 681.9
- 文献标志码:
- B
- 摘要:
- 以铁皮石斛未萌发原球茎、萌发原球茎为繁殖体，利用蔗糖、淀粉、ABA、NAA、活性炭、多菌灵、海藻酸钠设计了36种人工胚乳并研究它们对铁皮石斛人工种子萌发及生长状态的影响，筛选出适宜的繁殖体和胚乳配方，在此基础上进行包衣制作。结果表明：以萌发原球茎为繁殖体的人工种子萌发率和成活率较高。适宜的人工胚乳组合为蔗糖3.0％、ABA 1.0 mg/L、活性炭浓度0.5％、多菌灵3.0％、海藻酸钠3.0％。在铁皮石斛人工种子外层制作包衣，自然条件下可获得较高的萌发率和成活率。
- Abstract:
- The artificial seeds of Dendrobium candidum Wall ex Linldl. were produced with protocorm-like bodies and 36 kinds of prescription of artificial endosperm composed of sucrose, starch, ABA, NAA, activated carbon, carbendazim, sodium alginate. The results showed that the germination and survival rate of artificial seeds with the germinated protocorm-like bodies were higher than with the ungerminated pLBs. Appropriate prescription of artificial endosperm was composed of 3.0% sugar, 1 mg/L ABA, 0.5% activated carbon, 3.0% carbendazim and 3.0% sodium alginate. Synthetic artificial seeds after coated, the germination and survival rate of the synthetic artificial seeds of Dendrobium candidum got higner under natural conditions.
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备注/Memo
第一作者简介：曾颖苹（1982-），女，在读硕士，现主要从事植物组织培养等研究工作。