MA Guang,LIANG Fei-fei,GUO Ji-ping,et al.Silico Cloning and Sequence Analysis of F3H Gene of Chinese Cabbage[J].Northern Horticulture,2012,36(17):116-118.
白菜黄烷酮-3-羟化酶基因的电子克隆与序列分析
- Title:
- Silico Cloning and Sequence Analysis of F3H Gene of Chinese Cabbage
- 文章编号:
- 1001-0009（2012）17-0116-03
- Keywords:
- Flavanone 3-hydroxylase; Chinese cabbage; in silico cloning
- 分类号:
- S 682.36
- 文献标志码:
- A
- 摘要:
- 现根据同源基因相对保守性，利用已知的油菜F3H基因序列，采用电子克隆的方法获得了白菜F3H基因的cDNA序列；并采用生物信息工具对白菜F3H的性质包括氨基酸序列组成，物理和化学性质，疏水性或亲水性，二级和三级结构的特点进行了研究。结果表明：白菜F3H基因cDNA全长为1 228 bp，包含1个完整的开放读码框（1 077 bp），编码358个氨基酸。其分子量为40 104.6 Da，等电点为5.45；其三维结构显示其是具有7个螺旋和12个折叠和一些不规则卷曲组成的球状结构。
- Abstract:
- Flavanone 3-hydroxylase (F3H)，a critical enzyme，catalyzes an step in anthocyanin biosynthesis.According to the conservation of homologous gene，with F3H gene in Brassica napus,the cDNA of F3H gene in Chinese cabbage was in silico cloned.Some characters of the F3H，such as the composition of amino acid sequences，physical and chemical properties，hydrophobicity or hydrophilicity，secondary and tertiary structure of the protein and function were analyzed and predicted by the tools of bioinformatics.The results showed that the length of cloned F3H cDNA was 1 228 bp and it contained a complete ORF (1 077 bp)，encoded 358 amino acids.The calculated molecular weight of it was 40 104.6 Da，theoretical pI of 5.45.It was a bulbous structure with 7 helices，12 sheets，and some irregular coils in tertiary structure.
参考文献/References:
[1]Forkmann G，Heller W，Grisebach H.Anthocyanin biosynthesis in flowers of Matthiola incana.Flavanone 3-and flavonoid 3′-hydroxylases[J].Z Naturforsch Sect C Biosci，1980，35:691-695. [2]Britsch L，Ruhnau-Brich B，Forkmann G.Molecular cloning，sequence analysis，and in vitro expression of flavanone 3 beta-hydroxylase from Petunia?hybrida[J].J Biol Chem,1992，267:5380-5387. [3]Shen G A，Pang Y Z，Wu W S，et al.Cloning and Characterization of a Flavanone 3-Hydroxylase Gene from Ginkgo biloba[J].Biosci Rep，2006，26:19-29. [4]Xu B B，Li J N，Zhang X K，et al.Cloning and molecular characterization of afunctional flavonoid 3′-hydroxylase gene from Brassica napus[J].J Plant Physiol,2007，164：350-363. [5]马光.芜菁花青素合成关键基因F3H启动子区序列及蓝光受体CRY1基因的克隆与初步功能分析[D].哈尔滨:东北林业大学，2008. [6]Zhang H M，Jiang M G，Feng Y J.In silico cloning of MgEno-1 cDNA from Magnaporthe grisea[J].China J Bioinformatics，2005(4)：57-61.
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备注/Memo
第一作者简介：马光（1977-），男，山东济宁人，讲师，研究方向为植物生理学。E-mail：maaohan@163.com.