SONG Yang-bo,LIU Yan-lin,GENG Wan-gang.Comparison of Three Optimized Method for Isolating Total RNA from Grapevine Tissues[J].Northern Horticulture,2012,36(15):119-122.
三种提取酿酒葡萄不同组织总RNA的方法比较
- Title:
- Comparison of Three Optimized Method for Isolating Total RNA from Grapevine Tissues
- 文章编号:
- 1001-0009（2012）15-0119-04
- Keywords:
- grapevine; total RNA; isolation; optimized SDS-phenol method; optimized CTAB method; optimized Trizol method
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 通过对比改良SDS-酚法、改良CTAB法和改良Trizol法3种RNA提取方法，提取“赤霞珠”(‘Cabernet Sauvignon’)葡萄芽、叶片和果皮RNA的质量及浓度，并进一步分离、纯化得到较高质量的RNA。结果表明：改良SDS-酚法与改良CTAB法适用于芽和叶片的提取，可以获得较好质量、高浓度的总RNA，其28S与18S亮度极高，5S清晰可见，完整性较好；改良Trizol操作环节较少，省时，质量好，条带锐利，可清晰看出28S与18S亮度比为2∶1，虽提取的样品浓度较低，但足以供下一步研究使用。
- Abstract:
- In order to choose the best optimized method for isolating total RNA from grapevine tissues，the effects of optimized SDS-phenol method，optimized CTAB method and optimized Trizol method were compared by isolating total RNA from the grapevine buds，leaves and ripe berry skins.The results showed that quality and high cencentrition RNA were harvested by the optimized SDS-phenol method and the optimized CTAB method and the two methods are more suitable for RNA isolation from leaves and buds.At the same time，quality but less cencentrition RNA were gotten by the optimized Trizol method，while the total RNA was still enough for further research.
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备注/Memo
第一作者简介：宋洋波(1985-)，男，硕士，研究方向为酿酒酵母基因工程。