[1]马丽源,张瑛,尚福强,等.油松未成熟合子胚离体培养诱导胚性愈伤组织[J].北方园艺,2012,36(07):126-129.
　MA Li-yuan,ZHANG Ying,SHANG Fu-qiang,et al.Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulaeformis[J].Northern Horticulture,2012,36(07):126-129.
点击复制

油松未成熟合子胚离体培养诱导胚性愈伤组织

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年07期 页码: 126-129 栏目: 出版日期: 2012-07-16
Title:
Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulaeformis
文章编号:
1001-0009（2012）07-0126-04
作者:
马丽源1; 张瑛1; 尚福强1; 2; 姚荣升2; 崔建国1
1.沈阳农业大学 林学院，辽宁 沈阳 110866；
2.辽宁省森林经营研究所，辽宁 丹东 118002
Author(s):
MA Li-yuan1; ZHANG Ying1; SHANG Fu-qiang1; 2; YAO Rong-sheng2; CUI Jian-guo1
1.College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866；
2.Liaoning Institute of Forest Management，Dandong，Liaoning 118002
关键词:
油松; 胚性愈伤组织; 未成熟合子胚; 体胚发生
Keywords:
Pinus tabulaeformis; embryogenic calluses; immature zygotic embryo; somatic embryogenesis
分类号:
S 791.254
文献标志码:
A
摘要:
研究了油松未成熟合子胚不同发育时期和不同植物生长调节剂组合对油松胚性愈伤组织诱导的影响。结果表明:在沈阳地区，油松未成熟合子胚诱导胚性愈伤组织的最佳采种时间为7月10日左右；以DCR为基本培养基，添加较低浓度的KT和较高浓度的2,4-D有利于油松胚性愈伤组织的诱导，其中以添加2,4-D 2 mg/L和KT 0.5 mg/L时诱导率最高，可达 88.3%。
Abstract:
Effects of different developmental stages of immature zygotic embryo of Pinus tabulaeformis and different combination of plant growth regulators on induction of emvryogenic calluses were studied.The results showed that the optimal time of sampling the immature zygotic embryo for induction of embryogenic calluses in Shenyang region of China was around 10th of July；and when DCR was adopted as the basal medium，combination of low concentration of KT and high concentration of 2,4-D was favorable the induction of embryogenic calluses.The highest initiation rate was up to 88.3% on DCR medium when 2 mg/L of 2,4-D and 0.5 mg/L of KT were added.

参考文献/References:

[1]孙志强,孙占育,席梦利.针叶树体细胞胚胎发生研究进展[J].林业科技开发,2010,24(4):1-5.

[2]温伟,崔建国,张罡,等.松属树种体胚发生研究进展[J].辽宁林业科技,2010(3):44-47.
[3]祝朋芳,罗凤霞,陈长青,等.日本落叶松胚性愈伤组织的诱导及体胚的形成[J].辽宁农业科学,2001(5):46-47.
[4]贺凤美,王力,刘阳.落叶松体细胞发生研究进展[J].辽宁林业科技,2000(6):33-35.[5]郑均宝,王进茂,杜克久,等.油松体细胞无性系的建立[J].遗传学报,2000,24(1):1-6.[6]唐巍.火炬松胚性愈伤组织诱导和植株再生的研究[J].林业科学,1998,4(3):115-119.
[7]周巧云,郑彩霞,赵程亮.油松种胚愈伤诱导的初探[J].江西农业大学学报,2007,29(3):409-412.
[8]董丽芬,肖颖,邵崇斌.氮、磷、钾元素形态配比及浓度对油松胚培养的影响[J].西北林学院学报,2006,21(3)：64-66.
[9]贺娜,郑彩霞,周巧云.油松成熟胚的不定芽诱导及植株再生试验[J].现代农业科技,2009(16):145-148.
[10]李慧,张茜,付文锋.离体条件下油松成熟合子胚不定芽的形成[J].北京林业大学学报,2010,32(5):111-115.
[11]Kong D M，Wan T.Direct organogenesis and plantlet regeneration from mature zygotic embryos of Chinese pine (Pinus tabulaeformis Carr.) [J].Bulletin of Botanical Research，2010，30(6):668-673.[12]王永波,唐德瑞,马佩.培养基和生长调节剂对油松离体胚不定芽诱导的影响[J].北方园艺,2010(12)：149-151.
[13]温伟,林梅,魏爽,等.油松成熟胚的不定芽诱导及植株再生[J].辽宁林业科技,2010(5):17-18.
[14]张朝军,李付广,张玲.植物体细胞胚胎发生机理的研究进展[J].棉花学报,2008,20(2):141-147.
[15]崔凯荣,陈克明,王晓哲,等.植物体细胞胚发生研究的某些现状[J].植物学通报,1993,10(3):14-20.
[16]吴丽君,叶健仁.火炬松体胚发生与几种专利培养基的应用[J].南京林业大学学报,2008，32(1):1-5.
[17]吴丽君.长叶松优良家系的体胚发生研究[J].福建林学院学报,2008,28(1):42-47.[18]Pullman G S,Bucalo K,John M W.Pine somatic embryogenesis using zygotic embryos as explants [M]// Thorpe T A,Yeung E C.Plant Embryo Culture：Methods and Protocols.New York:Humana Press，2011:267-285.
[19]Jones N B，van Stadent J.Plantlets production from somatic embryo of Pinus patula[J].Journal of Plant Physiology，1995，145：519-525.
[20]Mott R L，Amerson H V，Frampton J.Annual progress report：Special project on tissue culture [J].Agriculture Research Service，1987：145.
[21]吴涛,陈少瑜,陈芳,等.思茅松胚性愈伤组织的诱导[J].中南林业科技大学学报,2007,27(5):74-78.
[22]Ellisd D.Stable transformation of Picea glauca by particle acceleration [J].Bio.Technology，1993,11:84-89.
[23]唐巍,欧阳藩,郭仲琛.火炬松胚性愈伤组织诱导和植株再生的研究[J].林业科学,1998,34(3):115-119.
[24]李茜,张存旭,郑瑞杰.白皮松胚性愈伤组织诱导因素的研究[J].西北林学院学报,2006,21(2):80-83.

相似文献/References:

[1]涂永成,裴宗平,周 江,等.八种抗旱材料组合对干旱矿区油松抗旱性的影响[J].北方园艺,2014,38(09):63.
　TU Yong-cheng,PEI Zong-ping,ZHOU Jiang,et al.Effect of Eight Kinds of Drought-resistant Materials Combination on Drought Resistance of Pinus tabulaeformis in Dry Mining Area[J].Northern Horticulture,2014,38(07):63.
[2]曹亚琼,张存旭.侧柏胚性愈伤组织的诱导研究[J].北方园艺,2013,37(06):108.
　CAO Ya-qiong,ZHANG Cun-xu.Study on Induction of Embryogenic Tissue of Platycladus orientalis [J].Northern Horticulture,2013,37(07):108.
[3]代月,吴雄,廉美兰,等.影响洋葱愈伤组织及胚性愈伤组织诱导的因素研究[J].北方园艺,2013,37(07):125.
　DAI Yue,WU Xiong,LIAN Mei-lan,et al.Study on the Factors of Onion Callus and Embryonic Callus Induction[J].Northern Horticulture,2013,37(07):125.
[4]王虎,樊军锋,杨培华,等.油松二代种子园遗传多样性RAPD分析[J].北方园艺,2012,36(04):117.
　WANG HU,FAN Jun-feng,YANG Pei-hua,et al.Genetic Diversity Analysis on Second-generation Seed Orchard of Pinus tabulaeformis Carr.by RAPD Markers[J].Northern Horticulture,2012,36(07):117.
[5]魏 杰,闫 伟.油松外生菌根真菌名录[J].北方园艺,2015,39(03):126.[doi:10.11937/bfyy.201503037]
　WEI Jie,YAN Wei.A Checklist of Ectomycorrhizal Fungi of Chinese Pine[J].Northern Horticulture,2015,39(07):126.[doi:10.11937/bfyy.201503037]
[6]国润才,高永,黄昕.包头市不同生长状况油松光合速率对比研究[J].北方园艺,2015,39(19):69.[doi:10.11937/bfyy.201519017]
　GUO Runcai,GAO Yong,HUANG Xin.Comparative Study on Photosynthetic Rate of Pinus tabulaeformis Carr. in Different Growth Conditions of Baotou City[J].Northern Horticulture,2015,39(07):69.[doi:10.11937/bfyy.201519017]
[7]陈 建 中,葛 水 莲,杨 明 建.太行山东麓油松种子园遗传多样性的 RAPD 分析[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007051]
　CHEN Jian -zhong,GE Shui-lian,YANG Ming -jian.Genetic Diversity Analysis on SeedOrchard of Pinus tabulaeformis in the Southern T aihang Mountainousby RAPD Markers[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.201007051]
[8]王 永 波,唐 德 瑞,马 　 佩.培养基和生长调节剂对油松离体胚不定芽诱导的影响[J].北方园艺,2010,34(12):0.[doi:10.11937/bfyy.2010012057]
　W ANG Yong -bo,TANG De -rui,MA Pei.The Influence of Medias and G rowth Regulators on Explants to Induce Adventitious Buds of Pinustabulaeformis[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.2010012057]
[9]马 　 佩,唐 德 瑞,王 永 波.油松成熟胚子叶-胚轴段的离体培养与不定芽诱导[J].北方园艺,2011,35(07):0.[doi:10.11937/bfyy.201107042]
　MA Pei,TANG De -rui,W ANG Yong -bo.In Vitro Formation of Adventitious Buds From the Embryos’ Cotyledon-epicotyl of Pinus tabulaeformis and Adventitious Buds Culture[J].Northern Horticulture,2011,35(07):0.[doi:10.11937/bfyy.201107042]
[10]樊 永 军,等.油松外生菌根真菌对其幼苗根际其它真菌的影响[J].北方园艺,2009,33(02):0.[doi:10.11937/bfyy.200902009]
　FAN Yong -jun,YAN Wei,W ANG Li -yuan.The Research on the Effect of Ectomycorrhizal Fungi of PinusTabulaeformis on Other Rhizosphere Fungi of It’ s Young Seedings[J].Northern Horticulture,2009,33(07):0.[doi:10.11937/bfyy.200902009]

备注/Memo

第一作者简介：马丽源(1986-)，女，硕士，现从事林木生物技术研究工作。E-mail：mly_19861221@yahoo.com.cn。

责任作者：崔建国（1969-），男，博士，教授，现从事林木生物技术和林木遗传改良研究工作。E-mail：cjgsau@yahoo.com.cn。
收稿日期：2011-12-21

更新日期/Last Update: 2014-09-03