SUN Jing-di,WEI Xiao-wei,CAI Rui,et al.The Regulation of Regeneration on Tomato Genotypes by Different Hormone Combinations[J].Northern Horticulture,2012,36(05):115-118.
激素配比对不同基因型番茄再生体系的调控
- Title:
- The Regulation of Regeneration on Tomato Genotypes by Different Hormone Combinations
- 文章编号:
- 1001-0009（2012）05-0115-04
- Keywords:
- tomato; genotype; hormone combinations; regeneration
- 分类号:
- Q 943.1
- 文献标志码:
- A
- 摘要:
- 以“粉桃”、“春秀”、“中蔬四号”、“齐研矮粉”、“红润”5种基因型番茄品种为试材,取7 d的无菌苗子叶作为外植体，研究不同激素配比对不同基因型番茄愈伤组织、不定芽及根诱导的影响。结果表明：不同基因型番茄再生体系受不同激素配比的调控差异显著。筛选得出愈伤组织诱导最佳培养基为：MSB5+6-BA 2.0 mg/L+IAA 0.2~0.5 mg/L；不定芽诱导最佳培养基为： MSB5+6-BA 2.0 mg/L+IAA 0.15~0.50 mg/L；不定芽生根最佳培养基为：1/2MS+IBA 0.5 mg/L。
- Abstract:
- Taking five genotypes of tomato: ‘Pink peach’，‘Chunxiu’，‘Zhongshu IV’，‘Qiyanaifen’,‘Red moist’ as materials,with cotyledons were cultured 7 days as explants,the effecet on different hormone combinations of different genotypes of tomato callus，adventitious bud and root-induced were studied.The results showed that the best medium for callus induction MSB5+6-BA 2.0 mg/L+IAA 0.2~0.5 mg/L；the best medium for adventitious bud induction: MSB5+6-BA 2.0 mg/L+IAA 0.15~0.50 mg/L;the best medium for rooting shoots 1/2MS+IBA 0.5 mg/L.Obtained through this research comparing different genotypes of tomato regeneration subject to regulation significantly by different of hormone ratio.
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备注/Memo
第一作者简介：孙靖棣（1981-），女，在读硕士，研究方向为番茄遗传转化。E-mail: wxw0610616@126.com。
责任作者：周晓馥（1964-），女，博士，教授，硕士生导师，现主要从事植物基因工程的研究工作。E-mail: zhouxiaofu@jlnu.edu.cn。
基金项目：国家自然科学基金资助项目（30970219）。
收稿日期：2011-12-08