ZONG Chang-ling,ZONG Cheng-wen,ZHAO Wei-wei,et al.In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain[J].Northern Horticulture,2012,36(04):113-116.
长白山笃斯越桔优良单株组培快繁技术的研究
- Title:
- In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain
- 文章编号:
- 1001-0009(2012)04-0113-04
- 分类号:
- S 666.9
- 文献标志码:
- A
- 摘要:
- 以长白山笃斯越桔优良单株的茎尖、茎段为外植体,对其组培快繁技术进行了研究。结果表明:笃斯越桔的幼芽分化适宜的启动培养基为WPM+ZT 2.0 mg/L;增殖培养基为改良MS+ZT 0.5 mg/L+IBA 0.15 mg/L,继代培养30 d后的增殖倍数可达5.3倍;适宜的生根培养基为1/4改良MS+IBA 0.5 mg/L,培养50 d后的生根率可达80%。将笃斯越桔组培苗在纯沙子中练苗20 d,将苗移栽至草炭土∶腐殖土为1∶2的基质中,组培苗移栽成活率达78%。
- Abstract:
- Taking stem or shoot tip of Vaccinium uliginosum as the explants,in vitro culture of superior individual of Vaccinium uliginosum distributing in Changbai Mountain were studied.The results indicated that the media for inducing shoot clusters was WPM+ZT 2.0 mg/L;the medium for subculture multiplication culture was modified MS+ZT 0.5 mg/L+IBA 0.2 mg/L,and the coefficient of multiplication was 5.3 after culture for 30 days;the medium for the rooting was 1/4 MS+IBA 0.5 mg/L with rooting rate of over 80% after culture for 50 days.Practices transplanting seedling was to tissue culture in sand 20 days,and then transplanting seedlings to the turfy soil: humus soil 1∶2,and survival rate in nursery was up to 78%.
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备注/Memo
第一作者简介:宗长玲(1983-),女,吉林榆树人,在读硕士,现主要从事果树生物技术研究工作。E-mail:zongchangling0925@163.com。
责任作者:宗成文 (1974-),男,博士,副教授,现主要从事果树遗传育种与生物技术研究。E-mail:zongchengwen@yahoo.com.cn。
基金项目:国家自然科学基金资助项目(30960231)。
收稿日期:2011-11-29