[1]李庆玲,陈荣,杨跃生,等.两种天南星科植物根尖组织染色体观察方法的优化[J].北方园艺,2012,36(02):134-136.
　LI Qing-ling,CHEN Rong,YANG Yue-sheng,et al.Optimization of Chromosome Observing Method of Root-tip Tissue in Two Species of Araceae[J].Northern Horticulture,2012,36(02):134-136.
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两种天南星科植物根尖组织染色体观察方法的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年02期 页码: 134-136 栏目: 出版日期: 2012-02-16
Title:
Optimization of Chromosome Observing Method of Root-tip Tissue in Two Species of Araceae
文章编号:
1001-0009（2012）02-0134-03
作者:
李庆玲1; 陈荣1; 2; 杨跃生1; 朱昌叁2
1.华南农业大学 生命科学院药用植物研究中心，广东 广州 510642；
2.广西生态工程职业技术学院，广西 柳州 545004
Author(s):
LI Qing-ling1; CHEN Rong1; 2; YANG Yue-sheng1; ZHU Chang-san2
1.Center for Medicinal Plant Research,College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642;
2.GuangxiEco-engineering Vocational and Technical College,Liuzhou,Guangxi 545004
关键词:
白掌; 观音莲; 染色体观察
Keywords:
Spathiphyllum floribundum; Alocasia indica Schott; chromosome observation
分类号:
S 68236
文献标志码:
A
摘要:
以白掌和观音莲2种天南星科观赏植物为试材，对其根尖组织染色体观察方法进行优化。结果表明：2种材料均在12:00~14:00取材较好；对二氯苯的饱和液中预处理时，白掌以在8℃冰箱中预处理6 h效果好，观音莲在16℃环境下预处理8 h为最优条件；40℃恒温水浴锅中1 mol/L盐酸酸解处理时，白掌酸解10 min，观音莲处理15 min效果理想；最后切取根尖白色分生组织部分于载玻片上使用卡宝品红染色20 min可以满足染色体计数和拍照的需要。
Abstract:
Araceae plants Spathiphyllum floribundum and Alocasia indica Schott were used in this study to optimize the chromosome observation method of root-tip tissue.The results showed that both materials were better collected at the time period of 12:00~14:00.Spathiphyllum floribundum achieved better result with a pretreatment of 6 h in fridge at the temperature of 8℃ and Alocasia indica Schott reached optimum condition with a pretreatment of 8 h in fridge at the temperature of 16℃ when pretreated in saturated dichlorobenzene.Spathiphyllum floribundum and Alocasia indica Schott reached ideal results when respectively treated for 10 min and 15 min with HCl 1 mol/L for 40 min in water bath.White meristematic tissue at the root-tip was cut and put on glass slide for chromosome number observation and photographing after 20 min dyeing with carbolfuchsin.

参考文献/References:

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[2]杜宝贵,黄丽娟,张志胜,等.红掌花药培养[J].生物技术通报,2009（增刊）：189-195.
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备注/Memo

第一作者简介：李庆玲（1987-），女，在读硕士，现从事植物倍性育种研究工作。E-mail:chentianyigl@126.com。

责任作者：朱昌叁（1975-），男，硕士，工程师，现从事植物生物技术研究工作。
基金项目：广西自然科学基金资助项目（2010GXNSFB013024)。
收稿日期：2011-10-27

更新日期/Last Update: 2014-08-24