[1]丁兰,郭艳,董刚,等.国产蝴蝶兰种苗携带建兰花叶病毒(CymMV)和齿兰环斑病毒 (ORSV)的调查及脱毒的初步研究[J].北方园艺,2012,36(02):137-140.
　DING Lan,GUO Yan,DONG Gang,et al.Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China[J].Northern Horticulture,2012,36(02):137-140.
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国产蝴蝶兰种苗携带建兰花叶病毒(CymMV)和齿兰环斑病毒 (ORSV)的调查及脱毒的初步研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年02期 页码: 137-140 栏目: 出版日期: 2012-02-16
Title:
Investigation and Preliminary Study of Elimination of CymMV and ORSV from Phalaenopsis in China
文章编号:
1001-0009（2012）02-0137-04
作者:
丁兰; 郭艳; 董刚; 杨玲; 张丽; 刘国安
西北师范大学 生命科学学院,甘肃 兰州 730070
Author(s):
DING Lan; GUO Yan; DONG Gang; YANG Ling; ZHANG Li; LIU Guo-an
College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070
关键词:
DAS-ELISA; 蝴蝶兰; 建兰花叶病毒; 齿兰环斑病毒; 脱毒
Keywords:
DAS-ELISA; Phalaenopsis; Cymbidium mosaic virus; Odontoglossum ringspot virus; virus-free
分类号:
S 68231
文献标志码:
A
摘要:
采用双抗夹心酶联免疫吸附法(DAS-ELISA)对国内不同蝴蝶兰种植场的17个蝴蝶兰分生苗品系种苗（22个样本）携带的2种主要病毒(CymMV和ORSV)的情况进行了检测调查，并采用茎尖培养和原球茎诱导的方式对携带病毒的4个蝴蝶兰品系进行了脱毒研究。结果表明：22个样本中有20个样本复合感染2种病毒，仅有1个样本显示了阴性结果。采用茎尖培养的‘R-2-2’和‘Y-4-2’品系，其脱毒率分别为227%(CymMV)和191%(ORSV)，303%(CymMV)和80%(ORSV)；采用原球茎脱毒的‘R-1-2’和‘R-11-1’品系，其脱毒率分别为254%(CymMV)和14% (ORSV)，252%(CymMV)和242% (ORSV)。茎尖培养和原球茎诱导方式均不能一次性彻底脱毒。
Abstract:
The study had detected two main virus,Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) of seventeen strains（twenty two samples） of Phalaenopsis collected from different farms,with the method of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA);Meanwhile,two ways were employed for Elimination of the viruses from four Phalaenopsis.The results showed that twenty samples were positive for two viruses (CymMV and ORSV),and only one sample was negative for two viruses (CymMV and ORSV).The virus-free rate of strain R-2-2 and strain Y-4-2 were 227% (CymMV) and 191% (ORSV),303% (CymMV) and 80% (ORSV) after shoot-tip culture,respectively;the virus-free rate of strain R-1-2 and strain R-11-1 were 254% (CymMV) and 14% (ORSV)，252% (CymMV) and 242% (ORSV) by induction protocorm,respectively.It indicated that the viruses from Phalaenopsis could not be eliminated thoroughly by shoot-tip culture or induction protocorm for only one time.

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备注/Memo

第一作者简介：丁兰（1964-），女，四川德阳人，博士，教授，现从事植物细胞工程及分子药理学研究工作。E-mail:dinglan@nwnu.edu.cn。

基金项目：国家自然科学基金资助项目(30960464)；西北师范大学知识与科技创新工程资助项目（NWNU-KJCXGC-03-65）。
收稿日期：2011-10-14

更新日期/Last Update: 2014-08-24