[1]王静,麻冬梅,许兴.苜蓿高频再生体系的建立[J].北方园艺,2012,36(02):109-112.
　WANG Jing,MA Dong-mei,XU Xing.Establishment of High Frequency Regeneration System of Alfalfa[J].Northern Horticulture,2012,36(02):109-112.
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苜蓿高频再生体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年02期 页码: 109-112 栏目: 出版日期: 2012-02-16
Title:
Establishment of High Frequency Regeneration System of Alfalfa
文章编号:
1001-0009(2012)02-0109-04
作者:
王静1; 麻冬梅2; 许兴2
1.宁夏大学 生命科学学院,宁夏 银川 750021;
2.宁夏大学 农学院,宁夏 银川 750021
Author(s):
WANG Jing1; MA Dong-mei2; XU Xing2
1.College of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021;
2.College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021
关键词:
苜蓿; 外植体; 愈伤组织; 高频再生体系
Keywords:
alfalfa; explant; callus; high frequency regeneration system
分类号:
S 551+7
文献标志码:
A
摘要:
以10个品种苜蓿无菌苗的子叶和下胚轴为外植体,研究了不同培养基、不同激素种类和配比对愈伤组织诱导率及分化率的影响。结果表明：国产苜蓿下胚轴外植体愈伤组织分化率最高；愈伤组织诱导最适培养基为MS盐+UM维生素+2 mg/L 2,4-D+025 mg/L KT+2 g/L水解酪蛋白+6 g/L琼脂+30 g/L蔗糖；愈伤组织最适分化培养基为MS+05 mg/L KT+01 mg/L NAA；最适生根培养基为1/2MS。
Abstract:
The experiment gets across using ten kinds of the hypocotyl and cotyledon of alfalfa as explants were used to study the effect of different mediums,hormones in different concentrations on callus induction rate and differentiation rate.The results indicated that the Medicago sativa L.cv.Guochan hypocotyl callus differentiation rate was the highest;the best medium for callus induction was the MS salt+UM vitamin,2.0 mg/L 2,4-D+025 mg/L KT,2.0 g/L casein acid hydrolysate+6 g/L Agar+30 g/L sucrose;the best differentiation medium was the MS medium+05 mg/L KT+01 mg/LNAA;the best root medium for plant development was 1/2 MS.

参考文献/References:

[1]从学滋,秦孟根,王书安.苜蓿总皂甙的提取与分析[J].中药通报,1998,13(9):19-20.

[2]David A,Stuart,Carol M,McCall Induction of somatic embryogenesis usingside chain and ring modified forms of phenoxy acid growth regulators[J].Plant Physiol.,1992,99:111-118.
[3]杨广东,朱祯,李燕娥,等.大白菜高效遗传转化体系的建立[J].农业生物技术学报,2002,10(2):127-132.
[4]危晓薇,蔡丽娟,李仁敬.紫花苜蓿组织培养及其再生植株[J].新疆农业科学,1999(2):73-75.
[5]刘淑兰,罗松,王天原,等.苜蓿组织培养及不定根芽分化蛋白质的研究[J].北京农业大学学报,1993(7):35-39.[6]刘明志.大叶紫花苜蓿愈伤组织原生质体再生植株[J].武汉植物学研究,1996,14(4):329-333.
[7]舒文华,耿华珠,阎伦兴.紫花苜蓿胚轴愈伤组织培养与植株再生[J].草业科学,1993,10(3):65-67.
[8]Jin T C,Chang Q,Li W F,et al.Stress-inducible expression of GmDREB1 conferred salt tolerance in transgenic alfalfa[J].Plant Cell Tiss Organ Cult,2010,100:219-227.
[9]李聪,熊德邵,耿华珠.苜蓿愈伤组织再生植株研究[J].中国草地,1989(3):51-55.
[10]王涌鑫,关宁,李聪.高效的苜蓿组织培养再生体系的建立[J].东北师范大学学报(自然科学版),2008(3):112-117.
[11]吕冬霞,曲长福.植物生长调节剂对愈伤组织培养的影响[J].北方园艺,2004(5):68-72.
[12]Ana S D,Ana S P,Dulce M D S,et al.Efficient somatic embryogenesis and plant regeneration from long-term cell suspension cultures of Medicago truncatula cv.Jemalong In Vitro xellular and developmental[J].Biology-Plant,2006,42(3):270-273.

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备注/Memo

第一作者简介：王静（1988-），女，宁夏银川人，硕士，现从事植物基因工程研究工作。E-mail：fengqingyangzi@163.com。

责任作者：许兴(1959-),男,教授,博士生导师,现从事作物逆境生理及生物技术育种研究工作。
基金项目：“十一五”国家科技支撑计划资助项目(2011BAC07B03)。
收稿日期：2011-10-08

更新日期/Last Update: 2014-08-24