WANG Gang,WEI Rong.Molecular Markers and Application of Seedless Grape Gene[J].Northern Horticulture,2014,38(04):178-181.
葡萄无核基因分子标记及应用
- Title:
- Molecular Markers and Application of Seedless Grape Gene
- 文章编号:
- 1001-0009(2014)04-0178-04
- Keywords:
- grape; seedless; molecular marker; breeding
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 无核葡萄是鲜食与制干葡萄的重要组成部分。当前市场上无核葡萄品种较少的主要原因是无核葡萄抗病性差、果粒小、产量低,因此优质无核葡萄品种选育成为当前葡萄育种领域的重要课题。育种过程中有核后代所占比例较大及童期较长在一定程度上制约无核葡萄育种的进度。无核基因分子标记技术的引入可以高效的在幼苗期检测出该品系是否为无核葡萄,有效的排除有核后代,加速无核葡萄育种进程。该文主要从葡萄无核标记的研究进展、在科研实践中的应用、存在问题以及未来发展方向等研究进行了综述。
- Abstract:
- Seedless grape (Vitis viniferaL.) is a great part in grapes produced for fresh and raisin consumption.There exist few varieties of seedless grapes in current market due to poor disease resistance,small fruits and low yield.So selecting high-quality seedless grape has become an important issue in grape breeding.The breeding progress is somewhat hindered by a large percentage of offspring made up by seeded grapes,and its long juvenile phase.Applying marker assisted selection (MAS) in breeding process can effectively discern and eliminate seeded germplasm,thus facilitate the breeding for seedless grapes.Research advances,problems and future directions of seedless grape molecular marker were reviewed in this paper.
参考文献/References:
[1]Oiv.Statistical report on world vitiviniculture[R].International Organisation of Vine and Wine,2012. [2]Cain D W,Emershad R L,Tarallo R E.In-ovulo embryo culture and seedling development of seeded and seedless grapes (Vitis viniferaL.)[J].Vitis,1983,22:9-14. [3]Doligez A,Bouquet A,Danglot Y,et al.Genetic mapping of grapevine (Vitis viniferaL.) applied to the detection of QTLs for seedlessness and berry weight[J].Theor Appl Genet,2002,105:780-795. [4]Uri H,Margarita V,Etti O,et al.Silencing of Chaperonin 21,that was differentially expressed in inflorescence of seedless and seeded grapes,promoted seed abortion in tobacco and tomato fruits[J].Transgenic Res,2007(16):515-525. [5]Uri H,Margarita V,Nachman S,et al.The ubiquitin extension protein S27a is differentially expressed in developing flower organs of Thompson seedless versus Thompson seeded grape isogenic clones[J].Plant Cell Rep,2009,28:1033-1042. [6]Wang S Y,Wang Y J,Zhang C H,et al.Molecular cloning and characterization of a novel gene encoding and EF-hand calcium-binding protein related to fruit seedlessness of grapevine[J].Scientia Hortculturae,2011,130:708-714. [7]Mejía N,Soto B,Guerrero M,et al.Molecular,genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine[J].BMC Plant Biology,2011(11):57. [8]Stout A B.Breeding for hardy seedless grapes[J].Proc Amer Soc Hort Sci,1937,34:416-420. [9]Bozhinova-Boneva I.Inheritance of seedlessness in grapes[J].Genet Sel,1978(11):399-405. [10]Loomis N H,Weinberger J H.Inheritance studies of seedlessness in grape[J].J Amer Soc Hort Sci,1979,104:181-184. [11]Roytchev V.Inheritance of grape seedlessness in seeded and seedless hybrid combinations of grape cultivars with complex genealogy[J].Am J Enol Vitic,1998,49:302-305. [12]Cabezas J A,Cervera M T,Garcia L R,et al.A genetic analysis of seed and berry weight in grapevine[J].Genome,2006,49:1572-1585. [13]Striem M J,Roy P S,Sahar N.The degree of development of the seed coat and the endosperm as separate subtraits of stenospermocarpic seedlessness in grapes[J].Vitis,1992,31:149-155. [14]Striem M J,Hayyim G B,Roy P S.Developing molecular markers for grape breeding,using polymerase chain reaction procedures[J].Vitis,1994,33:53-54. [15]Striem M J,Hayyim G B,Roy P S.Identifying molecular genetic markers associated with seedlessness in grape[J].J Amer Soc Hort Sci,1996,121(5):758-763. [16]Bouquet A,Danglot Y.Inheritance of seedlessness in grapevine (Vitis viniferaL.)[J].Vitis,1996,35(1):35-42. [17]Striem M J,Roy P S,Baron I,et al.Genomic DNA fingerprinting of Vitis viniferaby the use of multi-loci probes[J].Vitis,1990,29:223-227. [18]Wang Y J,Lamikanra O.Analysis of sequencing the RAPD marker linked to seedless genes in grapes[J].Acta Univ Agric Boreali-occidentalis,1997,25(4):1-5. [19]王跃进,Lamikanra O.检测葡萄无核基因DNA探针的合成与应用[J].西北农林科技大学学报(自然科学版),2002,30(3):42-46. [20]杨英军,王跃进,周鹏,等.葡萄无核基因的SCAR标记及Southern blot分析[J].西北农林科技大学学报(自然科学版),2002,30(6):77-80. [21]杨克强,王跃进,张今今,等.用限制性酶切和Southern杂交对葡萄无核基因分子标记的分析[J].农业生物技术学报,2005,13(3):294-298. [22]杨克强,王跃进,张今今,等.无核葡萄基因定位与作图的研究[J].遗传学报,2005,32(3):297-302. [23]Lahogue F,This P,Bouquet A.Identification of a codominant scar marker linked to the seedlessness character in grapevine[J].Theor Appl Genet,1998,97:950-959. [24]Ryan F J,Ramming D W.Application of a molecular marker for berry seed size to two populations of grapevines (Vitissp.) developed in a breeding program[J].Hortscience,2005,40(4):1069. [25]Mejía N,Hinrichsen P.A new,highly assertive SCAR marker potentially useful to assist selection for seedlessness in table grape breeding[J].Acta Horticulturae,2003,603:559-564. [26]Korp?s A,Bar?nek M,Pidra M,et al.Behaviour of two SCAR markers for seedlessness within Central Eurapean Varieties of grapevine[J].Vitis,2009,48(1):33-42. [27]Adam-Bondon A F,Esnault F L,Bouquet A,et al.Usefulness of two SCAR markers for marker-assisted selection of seedless grapevine cultivars[J].Vitis,2001,40(3):147-155. [28]Karaagac E,Vargas A M,Andrés M T,et al.Marker assisted selection fro seedlessness in table grape breeding[J].Tree Genetics and Genomes,2012(8):1003-1015. [29]Bergamini C,Cardone M F,Anaclerio A,et al.Validation assay of p3_VvAGL11 marker in a wide range of genetic background for early selection of stenospermocarpy in Vitis viniferaL[J].Mol Biotechnol,2013,54:1021-1030. [30]Li Z J,Dhekney S A,Gray D J.Molecular characterization of a SCAR marker purportedly linked to seedlessness in grapevine (Vitis)[J].Mol Breeding,2010,25:637-644. [31]Yang K Q,Wang Y J,Zhang J J,et al.Analysis of restriction sites and Southern blotting of two molecular markers linked to grape seedless gene[J].Chinese Journal of Agricultural Biotechnology,2006,3(1):13-17. [32]Costantini L,Battilana J,Grando M S,et al.Berry and phenology-related traits in grapevine (Vitis viniferaL.):From Quantitative Trait Loci to underlying genes[J].BMC Plant Biology,2008(8):38-55. [33]Scott K D,Ablett E M,Lee L S,et al.AFLP markers distinguishing an early mutant of Flame Seedless grape[J].Euphytica,2000,113:245-249. [34]张剑侠,王勇,王跃进,等.利用分子标记对无核抗病葡萄杂交后代的辅助选择[J].东北农业大学学报,2010,41(6):55-63. [35]朱瑜,夏培蓓,伍新宇,等.木纳格葡萄胚挽救苗的获得及其无核性状分子鉴定[J].新疆农业大学学报,2012,35(1):22-24. [36]Akkurt M,Cakir A,Shidfar M,et al.Using SCC8,SCF27 and VMC7f2 markers in grapevine breeding for seedelessness via marker assisted selection[J].Genetics and Molecular Research,2012,11(3):2288-2294. [37]Akkurt M,Cakir A,Shidfar M,et al.Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg×Sultani progeny[J].Turk J Biol,2013,37:101-105. [38]Adam-blondon A F,Lahogue-esnault,Bouquet A,et al.Usefulness of two SCAR markers for marker-assisted selection of seedless grapevine cultivars[J].Vitis,2001,40(3):147-155.
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备注/Memo
第一作者简介:王刚(1989-),男,硕士研究生,现主要从事鲜食无核葡萄胚挽救育种等研究工作。E-mail:wanggang827@126.com.