ZHANG Ruigang,WANG Chaoyue.Optimization of Extraction Process and in vitro Lipid-lowering Activity of Seabuckthorn Leaf Tea Polysaccharides[J].Northern Horticulture,2024,(1):93-99.[doi:10.11937/bfyy.20231928]
沙棘叶茶多糖提取工艺优化及体外降脂活性研究
- Title:
- Optimization of Extraction Process and in vitro Lipid-lowering Activity of Seabuckthorn Leaf Tea Polysaccharides
- 文章编号:
- 1001-0009(2024)01-0093-07
- 分类号:
- S 793.6
- 文献标志码:
- A
- 摘要:
- 以沙棘叶茶为试材，采用单因素试验与正交实验相结合的方法，研究了料液比、超声时间、超声温度和乙醇浓度对多糖提取率的影响以及沙棘叶茶多糖在体外的降脂功能，以期为沙棘叶茶多糖的综合开发利用提供参考依据。结果表明：沙棘叶茶多糖的最优提取条件为料液比1∶20 g·mL-1，超声时间60 min，超声温度60 ℃，乙醇浓度85%，在此条件下，沙棘叶茶多糖的提取率为(5.277±0.149)mg·g-1。并且，沙棘叶茶多糖对胆固醇酯酶和胰脂肪酶的抑制率分别可达到70%和60%，且存在剂量-效应关系,说明沙棘叶茶多糖具有一定降脂效果。
- Abstract:
- Taking Hippophae rhamnoides leaf tea as test material，using the method of single factor experiment and orthogonal experiment to study the ratio of material to liquid，the effects of ultrasonic time，ultrasonic temperature and ethanol concentration on the extraction rate of polysaccharide and the lipid-lowering function of tea polysaccharide from Hippophae rhamnoides leaf in vitro were studied，in order to provide reference for comprehensive development and utilization of tea polysaccharide from hippophae rhamnoides leaf.The results showed that the optimal extraction conditions of sea buckthorn tea polysaccharide were 1∶20 g·mL-1，60 minutes sonication time，60 ℃ sonication temperature and 85% ethanol concentration，under which the extraction rate of sea buckthorn tea polysaccharide was (5.277±0.149)mg·g-1.Moreover，the inhibition rates of sea buckthorn tea polysaccharide on cholesterol esterase and pancreatic lipase could reach 70% and 60%，respectively，and the dose-effect relationship was observed，indicating that sea buckthorn tea polysaccharide has certain lipid-lowering effect.
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备注/Memo
第一作者简介：张瑞刚（1984-），男，博士，副教授，现主要从事植物天然活性物质提取及功能研究等工作。E-mail:164613361@qq.com.基金项目：山西省应用基础研究计划资助项目（201901D211447）。收稿日期：2023-06-05