TAO Gang,GAI Jiaxin,RUAN Shiqin,et al.Potential Impacts of MRE on the Growth Promotion of Pepper Seedling Mediated by Their Host Arbuscular Mycorrhizal Fungi[J].Northern Horticulture,2024,(1):1-8.[doi:10.11937/bfyy.20231779]
内共生细菌对宿主丛枝菌根真菌促进辣椒生长的潜在影响
- Title:
- Potential Impacts of MRE on the Growth Promotion of Pepper Seedling Mediated by Their Host Arbuscular Mycorrhizal Fungi
- 文章编号:
- 1001-0009(2024)01-0001-08
- Keywords:
- arbuscular mycorrhizal fungi; mollicutes/mycoplasma-related endobacteria; chlorophyll content; growth-promoting effect
- 分类号:
- S 476
- 文献标志码:
- A
- 摘要:
- 以有MRE和无MRE的4个AMF种类为试材，采用AMF单孢分离法、分子生物学方法以及活体接种方法，研究了MRE的分类地位，辣椒生长指标和叶绿素含量的变化情况，以期初步阐明MRE在AMF促进植物生长发育中的潜在作用和影响。结果表明：MRE属于Candidatus Moeniiplasma glomeromycotorum (CaMg) 类细菌；4种AMF均能促进辣椒植株生长，但不同种类处理间存在显著差异；与空白对照相比，有MRE的AMF在辣椒株高方面增加了11.11%～33.43%，辣椒叶片的叶绿素含量增加了65.38%～95.19%，无MRE的AMF在辣椒株高方面增加了20.45%，辣椒叶片中叶绿素含量增加了60.58%；其中有MRE的明根孢囊霉 (Rc) 对辣椒株高和叶绿素含量影响最显著，与无MRE的根内根孢囊霉 (Ri) 相比，明根孢囊霉 (Rc) 处理后辣椒株高增加了10.78%，叶绿素含量增加了21.56%。MRE在宿主AMF对辣椒植株生长作用中具有潜在影响，表明MRE是提高宿主AMF对辣椒生长发育促进作用的一个重要潜在因素，该研究结果为AMF在绿色农业实践和应用中提供参考依据。
- Abstract:
- Four species of AMF with and without MRE were selected as experimental materials to study the classification status of MRE，the changes of growth index and chlorophyll content of pepper by single spore isolation，molecular biology and in vivo inoculation，in order to preliminarily clarify the potential role and influence of MRE in promoting plant growth and development by AMF.The results showed that MRE belonged to Candidatus Moeniiplasma glomeromycotorum (CaMg).All the four AMFs could promote the growth of pepper plants，but there were significant differences among different treatments.Compared with blank control，AMF with MRE increased plant height by 11.11%-33.43% and chlorophyll content in pepper leaves by 65.38%-95.19%，AMF without MRE increased plant height by 20.45% and chlorophyll content in pepper leaves by 60.58%.The most significant effect of MRE on plant height and chlorophyll content of capsicum was found in Rc.Compared with Ri without MRE，plant height and chlorophyll content increased by 10.78% and 21.56% after Rc.The potential effect of MRE on the growth of pepper plants by host AMF indicates that MRE is an important potential factor to enhance the promoting effect of host AMF on the growth and development of pepper plants.The results of this study can provide reference for the practice and application of green agriculture by AMF.
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备注/Memo
第一作者简介：陶刚（1972-），男，博士，研究员，现主要从事植物真菌病害生物防治等研究工作。E-mail：ttg729@sina.com.基金项目：国家自然科学基金资助项目（31860520）；黔农科院国基后资助项目（［2021］37号）。收稿日期：2023-05-23