PENG Lyuchun,GE Baozhi,LI Xuejiao,et al.Growth and Physiological Responses to Alkali Stress and Alkali Tolerance Evaluation of Four Rhododendron Cultivars[J].Northern Horticulture,2023,(16):58-63.[doi:10.11937/bfyy.20230093]
四个高山杜鹃品种对碱胁迫的响应及其耐碱性评价
- Title:
- Growth and Physiological Responses to Alkali Stress and Alkali Tolerance Evaluation of Four Rhododendron Cultivars
- Keywords:
- Rhododendron; cultivar; alkali resistance; morphological indicators; physiological indicators
- 文献标志码:
- A
- 摘要:
- 以4个高山杜鹃园艺品种‘Cosmopolitan’‘Lord Roberts’‘Scintillation’‘Polaris’的试管苗为试材，采用试管内耐碱性评价的方法，通过形态指标（碱害指数、株高增长量、芽增殖数）观测和生理指标（可溶性糖含量、超氧化物岐化酶(SOD)活性）测定，研究了5个pH水平的培养基对高山杜鹃试管苗生长的影响，以期为高山杜鹃耐碱性室内评价提供参考依据。结果表明：4个高山杜鹃品种 中‘Cosmopolitan’耐碱性最强，培养基 中pH 8.9时，碱害指数为13，pH为8.1时，株高增长量显著高于其它处理；其次为‘Lord Roberts’，pH 8.9时，碱害指数为62，pH为4.8和7.0时，株高增长量显著高于其它处理；‘Scintillation’和‘Polaris’最不耐碱，pH 89时，碱害指数分别为97和100。除pH 89的处理外，同一品种在其余pH水平下芽增殖系数差异不大。‘Cosmopolitan’和‘Polaris’在pH 7.0时，SOD活性开始显著降低；‘Scintillation’则在pH 5.9时，SOD活性开始显著降低；而‘Lord Roberts’自pH 8.1开始，SOD活性已显著低于pH 4.8和5.9时。4个高山杜鹃品种为应对不断增强的碱胁迫在pH 8.1~8.9时，可溶性糖含量最高。
- Abstract:
- Taking the tube seedlings of four Rhododendron cultivars ‘Cosmopolitan’‘Lord Roberts’‘Scintillation’ and ‘Polaris’ as test materials，the effects of five pH levels of culture media on the growth of test tube seedlings of Rhododendron were studied by using the method of alkaline tolerance evaluation in vitro，through observation of morphological indicators(alkaline damage index，plant height growth，bud proliferation number) and determination of physiological indicators(soluble sugar content，superoxide dismutase (SOD) activity)，in order to provide reference for indoor evaluation of alkaline resistance of Rhododendrons.The results showed that among the four Rhododendron cultivars,‘Cosmopolitan’ had the strongest alkali resistance with an alkali damage index of 13 at pH 8.9,it′s plant height growth was significantly higher than other treatments at pH 8.1; next was ‘Lord Roberts’,with an alkali damage index of 62 at pH 8.9 and a significantly higher plant height growth at pH 4.8 and 7.0 compared to other treatments;‘Scintillation’ and ‘Polaris’ were the least alkali resistant,with an alkali damage index of 97 and 100 at pH 8.9,respectively. Except for the treatment of pH 8.9,there was no significant difference in bud proliferation coefficient for the same variety at other pH levels. At pH 7.0,the SOD activity of ‘Cosmopolitan’ and ‘Polaris’ began to significantly decrease,while at pH 5.9,the activity of SOD began to significantly decrease in ‘Scintillation’,since pH 8.1,the SOD activity of ‘Lord Roberts’ had been significantly lower than at pH 4.8 and 5.9. Four Rhododendron cultivars have the highest soluble sugar content in response to increasing alkaline stress at pH 8.1-8.9.
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备注/Memo
第一作者简介：彭绿春（1981-），女，硕士，副研究员，现主要从事杜鹃花繁育及种质创新等研究工作。E-mail：green315@126.com.责任作者：李世峰（1970-），男，硕士，研究员，现主要从事新型花卉植物的研究和开发等工作。E-mail：452977351@qq.com.基金项目：国家自然科学基金资助项目(31760230)；云南省种子种业联合实验室资助项目(202205AR070001);云南省科技厅科技计划生物种业和农产品精深加工专项资助项目（202302AE090018）。收稿日期：2023-01-09