HAN Meiling,ZHAO Yuheng,LI Houhua,et al.Analysis of Phenolic Material Accumulation and Flower Color Fading Forms of Different Malus spp.During Flowering Process[J].Northern Horticulture,2023,(16):64-70.[doi:10.11937/bfyy.20224807]
开花过程中不同海棠花瓣酚类物质动态变化与花色淡化原因分析
- Title:
- Analysis of Phenolic Material Accumulation and Flower Color Fading Forms of Different Malus spp.During Flowering Process
- Keywords:
- Malus spp.; flower color; polyphenol; anthocyanin
- 文献标志码:
- A
- 摘要:
- 以不同花色的海棠（‘绚丽’海棠、垂丝海棠、西府海棠、‘春喜’海棠和湖北海棠）为试材，采集不同开花时期的花瓣，观测其花色变化的过程，采用紫外分光光度计和高效液相色谱仪的方法测定花瓣酚类物质的含量和种类，研究了影响不同海棠花色差异和淡化的关键物质，以期为探究海棠花色淡化机制与花色育种提供参考依据。结果表明：5种海棠 中，‘绚丽’海棠的花色最红，开花过程 中，亮度L*值最低，总花青苷含量最高；湖北海棠花瓣在开花过程 中以白色为主，其L*值是最高的，总花青苷含量是极低的。因此花青苷含量的差异是引起海棠花色差异的关键原因。在开花过程 中，不同海棠花色显著淡化，总多酚、总黄酮、总花青苷含量均显著下降。花瓣 中含有丰富的花青苷、黄酮醇、黄烷酮、黄烷醇、二氢黄酮醇和酚酸类物质。其 中矢车菊素-3-O-半乳糖苷是海棠花瓣 中主要的花青苷，其含量在开花过程 中均显著降低，是引起花色淡化的主要物质。花瓣 中也检测到丰富的槲皮素-3-O-葡萄糖醛酸苷、金丝桃苷、绿原酸、根皮苷等物质，推测能够辅助花青苷呈色。
- Abstract:
- Five Malus spp.,including Malus ‘Radiant’,Malus halliana,Malus micromalus,Malus ‘Spring Sensation’,Malus hupehensis,were used as test materials.The petals at different blooming stages were collected,and the processes of flower color changing were observed.The contents and kinds of polyphenols in petals were detected through spectrophotometer and high performance liquid chromatography.The study objects are aimed at ascertaining the key substance influencing the flower color difference and fading of different Malus spp.,in order to provide reference for exploring the fading mechanism and breeding of flower color.The results showed that among five Malus spp.,the flower colors of Malus ‘Radiant’were the reddest,correspondingly,brightness L* values were the lowest and total anthocyanin contents were the highest.The flower colors of Malus hupehensis were nearly white during blooming process,correspondingly,L* values were the highest and total anthocyanin contents were extremely low.Therefore,the difference of anthocyanin contents was the key reason for the difference of Malus spp.flower colors.The flower colors of Malus spp.faded significantly,and total phenol contents,total flavonoid contents,and total anthocyanin contents decreased evidently during blooming process.There were abundant anthocyanins,flavonols,flavanols,flavonones,phenolic acids,and dihydroflavonols detected in petals .The cyanindin-3-O-galactoside was the main anthocyanin in Malus spp.petals,and its contents were significantly decreased during blooming process,which was the main subtance for the flower fading.Also,the high levels of quercetin-3-O-glucuronide,hyperoside,chlorogenic acid,and phloridzin were detected in petals.It was speculated that these polyphenols could assist anthocyanin color.
参考文献/References:
[1]HAN M L，YIN J，ZHAO Y H，et al.How the color fades from Malus halliana flowers:Transcriptome sequencing and DNA methylation analysis[J].Frontiers in Plant Science，2020(11):576054.[2]李元鹏，张英杰，朱志奇,等.遮荫对不同色系月季发育过程 中花色变化的影响[J].西北植物学报，2022，42(4):664-673.[3]LUO X，SUN D，WANG S，et al.Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony (Paeonia suffruticosa Andr.) flowers[J].Horticulture Research，2021，8(1):3242-3256.[4]YING L，YURY T，SCHOUTEN R E，et al.Anthocyanin biosynthesis and degradation mechanisms in solanaceous vegetables:A review[J].Frontiers in Chemistry，2018(6):52.[5]王业，闫亚丹，陶依玲，等.色素成分与理化因子对金花茶花瓣呈色的影响[J]. 中南林业科技大学学报，2022，11(21):1-13.[6]CUI L，HE N，ZHANG X，et al.Dynamic change of secondary metabolites and spectrum-effect relationship of Malus halliana koehne flowers during blooming[J].Nephron Clinical Practice,2018，16(1):362-370.[7]REHMAN R，YOU Y，ALI S，et al.Phenolic compounds as biochemical markers of senescence in woody ornamental flowers of Malus crabapple[J].Horticulture Environment & Biotechnology，2018，59:1-10.[8]张往祥，裘靓，江志华，等.观赏海棠品种群开花进程 中色素组分动态特征研究[J].园艺学报，2014，41(6):1145-1156.[9]FAN J，ZHANG W，ZHANG D，et al.Flowering stage and daytime affect scent emission of Malus ioensis ‘Prairie Rose’[J].Molecules，2019(24):2356.[10]WOLFE K，WU X Z，RUI H L.Antioxidant activity of apple peels[J].Journal of Agriculture and Food Chemistry，2003，51(3):609-614.[11]LI N，SHI J，WANG K.Profile and antioxidant activity of phenolic extracts from 10 crabapples(Malus wild species)[J].Journal of Agricultural and Food Chemistry,2014，62(3):574-581.[12]LI Y Y，MAO K，ZHAO C，et al.Mdcop1 ubiquitin e3 ligases interact with mdmyb1 to regulate light-induced anthocyanin biosynthesis and red fruit coloration in apple[J].Plant Physiology，2012，160(2):1011-1022.[13]QIN X X，LU Y F，PENG Z，et al.Systematic chemical analysis approach reveals superior antioxidant capacity via the synergistic effect of flavonoid compounds in red vegetative tissues[J].Frontiers in Chemistry，2018(6):9.[14]付林江，李厚华，李玲，等.金银花花色变化原因分析[J].林业科学，2013，49(10):155-161.[15]程怡，张云婷，王清明，等.月季花发育过程 中花色变化的生理生化研究[J].西北植物学报，2014(34):733-739.[16]REHMAN R，YOU Y，YANG C，et al.Characterization of phenolic compounds and active anthocyanin degradation in crabapple(Malus orientalis) flowers[J].Horticulture Environment Biotechnology，2017，58:324-333.[17]ZHAO X，HE F，ZHANG X K，et al.Impact of three phenolic copigments on the stability and color evolution of five basic anthocyanins in model wine systems[J].Food Chemistry，2022，375:131670.[18]PANDEY J，BASTOLA T，TRIPATHI J，et al.Estimation of total quercetin and rutin content in Malus domestica of nepalese origin by hplc method and determination of their antioxidative activity[J].Journal of Food Quality,2020，2020:8853426.[19]XUE L，WANG J，ZHAO J，et al.Study on cyanidin metabolism in petals of pink-flowered strawberry based on transcriptome sequencing and metabolite analysis[J].BMC Plant Biology,2019(19):423.[20]STOMPOR M，BRODA D，BAJEK-BIL A.Dihydrochalcones:Methods of acquisition and pharmacological properties,a first systematic review[J].Molecules，2019，24(24):4468.[21]ZENG X，LI H，JIANG W，et al.Phytochemical compositions，health-promoting properties and food applications of crabapples:A review[J].Food Chemistry，2022，386:132789.[22]TIAN L，CAO J，ZHAO T，et al.The bioavailability，extraction，biosynthesis and distribution of natural dihydrochalcone:Phloridzin[J].International Journal of Molecular Sciences，2021，22(2):962.[23]SU Z，JIA H，SUN M，et al.Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit[J].Frontiers in Nutrient，2022(9):961626.[24]王鹏，郭丽，刘爽，等.绿原酸提取物的抗氧化活性协同增效作用[J].北方园艺，2021(15):106-111.
相似文献/References:
[1]徐 慧,刘 超,钟汉东.不同光照强度对八仙花开花的影响[J].北方园艺,2014,38(01):81.
XU Hui,LIU Chao,ZHONG Han-dong.Effects of Different Light Intensity on the Blossom of Hydrangea macrophylla[J].Northern Horticulture,2014,38(16):81.
[2]王 婷.天津地区观赏海棠引种与筛选[J].北方园艺,2015,39(03):70.[doi:10.11937/bfyy.201503022]
WANG Ting.Introduction and Screening of Crabapple in Tianjin Region[J].Northern Horticulture,2015,39(16):70.[doi:10.11937/bfyy.201503022]
[3]丁灵,李崇晖,黄少华,等.调控观赏植物花色的类黄酮3′羟化酶基因研究进展[J].北方园艺,2015,39(17):188.[doi:10.11937/bfyy.201517049]
DING Ling,LI Chonghui,HUANG Shaohua,et al.Research Advances of Flavonoid 3′-Hydroxylase Gene in Ornamental Plant[J].Northern Horticulture,2015,39(16):188.[doi:10.11937/bfyy.201517049]
[4]刘小微,李厚华,王冰洁,等.AmAS1与Am4’CGT基因双价载体构建及在海棠花中的瞬时表达[J].北方园艺,2016,40(14):97.[doi:10.11937/bfyy.201614024]
LIU Xiaowei,LI Houhua,WANG Bingjie,et al.Construction of Recombinant Vector of AmAS 1 and Am4’CGT Genes and the Transient Expression in Malus Flowers[J].Northern Horticulture,2016,40(16):97.[doi:10.11937/bfyy.201614024]
[5]石 学 丽,刘 雅 平,罗 言 云.红色系观花植物的科属统计及其园林应用[J].北方园艺,2009,33(10):0.[doi:10.11937/bfyy.2009010070]
SHI Xue -li,LIU Ya -ping,LUO Yan -yun.The Statistics of Families and Classes for Red Flower Plants and the Application in Landscape Architecture[J].Northern Horticulture,2009,33(16):0.[doi:10.11937/bfyy.2009010070]
[6]韩江南,樊金玲,巩卫东,等.中原牡丹品种基于花色测定的聚类分析[J].北方园艺,2010,34(03):0.[doi:10.11937/bfyy.201003059]
HAN Jiang-nanI,FAN Jin-ling,GONG Wei-dong,et al.Cluster Analysis on Determination Flower Colors of Central Plains Tree Peony[J].Northern Horticulture,2010,34(16):0.[doi:10.11937/bfyy.201003059]
[7]李晓丽,庄得凤,马策,等.月季杂交后代花色遗传特性初探[J].北方园艺,2017,41(14):96.[doi:10.11937/bfyy.20164823]
(Liaoning Research Institute of Cash Crops,Liaoyang,Liaoning 000).Inheritance Characteristics of Flower Color in Rose Hybrid Offspring LI Xiaoli,ZHUANG Defeng,MA Ce,MA Dan,LI Zhentao[J].Northern Horticulture,2017,41(16):96.[doi:10.11937/bfyy.20164823]
[8]丁芳兵,孙伟博,原雅玲,等.百合花色及相关育种研究进展[J].北方园艺,2017,41(18):170.[doi:10.11937/bfyy.20170056]
DING Fangbing,SUN Weibo,YUAN Yaling,et al.Research on Flower Color and Breeding of Lilium[J].Northern Horticulture,2017,41(16):170.[doi:10.11937/bfyy.20170056]
[9]李晓颍,赵晴晴,刘月,等.海棠果实中脱落酸的高效液相色谱测定[J].北方园艺,2018,42(04):138.[doi:10.11937/bfyy.20172564]
LI Xiaoying,ZHAO Qingqing,LIU Yue,et al.Determination of Abscisic Acid in Crabapple by High Performance Liquid Chromatography[J].Northern Horticulture,2018,42(16):138.[doi:10.11937/bfyy.20172564]
[10]邢世通,韩冰,谷颐.重要观赏植物的花色改良[J].北方园艺,2018,42(09):166.[doi:10.11937/bfyy.20173362]
XING Shitong,HAN Bing,GU Yi.Genetic Improvement of Flower Color in Major Ornamental Plants[J].Northern Horticulture,2018,42(16):166.[doi:10.11937/bfyy.20173362]
[11]田涛,王晓叶,赵思思,等.绚丽海棠实生后代叶色及花色变化研究[J].北方园艺,2017,41(14):69.[doi:10.11937/bfyy.20170042]
TIAN Tao,WANG Xiaoye,ZHAO Sisi,et al.Study on the Changes of Leaf Color and Flowers Color of Malus chaenomeles[J].Northern Horticulture,2017,41(16):69.[doi:10.11937/bfyy.20170042]
备注/Memo
第一作者简介：韩美玲（1991-）,女，博士，讲师，现主要从事园林植物类黄酮合成途径等研究工作。E-mail:hml1215@sxau.edu.cn.责任作者：李厚华（1973-），男，博士，教授，现主要从事园林植物类黄酮次生代谢及转基因等研究工作。E-mail：lihouhua73@163.com.基金项目：山西省自然科学基金资助项目（20210302124687）；山西农业大学博士科研启动基金资助项目（2020BQ52）；国家自然科学基金资助项目（32171862）。收稿日期：2022-11-24