[1]周莹,袁孟娟,韩军,等.中药材丹参根腐病生防菌的分离与鉴定[J].北方园艺,2015,39(01):145-147.[doi:10.11937/bfyy.201501039]
　ZHOU Ying,YUAN Meng-juan,HAN Jun,et al.Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents[J].Northern Horticulture,2015,39(01):145-147.[doi:10.11937/bfyy.201501039]

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中药材丹参根腐病生防菌的分离与鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年01 页码: 145-147 栏目: 出版日期: 2015-01-15

Title:: Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents

作者:: 周莹 1; 袁孟娟 1; 韩军 1; 董惠钧 1; 藏香银 2; 陈芳 1; （1.聊城大学药学院，山东聊城 252059；2 山东东阿阿胶股份有限公司，山东聊城 252200）

Author(s):: ZHOU Ying 1; YUAN Meng-juan 1; HAN Jun 1; DONG Hui-jun 1; ZANG Xiang-yin 2; CHEN Fang 1; (1.College of Pharmacy，Liaocheng University，Liaocheng，Shandong 252059；2.Shandong Dong’e E Jiao Co.，Ltd.，Liaocheng，Shandong 252200)

关键词:: 关键词：丹参; 根腐病; 生防菌; 鉴定

Keywords:: Keywords: S.miltiorrhiza Bunge; root rot; biocontrol agents; identification

DOI:: 10.11937/bfyy.201501039

文献标志码:: A

摘要:: 摘要：以聊城市茬平县杜郎口镇中药材种植基地采集的丹参根际土壤为试材，采用梯度稀释法和平板对峙培养法在丹参根际土壤中筛选对丹参根腐病具有较好抑制作用的生防菌。结果表明：初步筛选出10株具有抑菌活性的有益微生物；进一步通过复筛及其抑菌谱的测定分离出2株抑菌效果较好的菌株，分别为2-1和9-2。通过形态学和分子生物学鉴定，2-1和9-2分别为枯草芽孢杆菌（Bacillus subtilis ）、解淀粉芽孢杆菌（Bacillus amyloliquefaciens ）,该试验可为今后高效生防菌剂的研发奠定基础。

Abstract:: Abstract:Taking rhizosphene soil of Chinese medicine S.miltiorrhiza Bunge root that from langkou town Chinese herbal medicine planting base in Chiping Connty Liaocheng city as material,used gradient dilution method and dual culture method,better inhibitory biocontrol agents to root rot were selected.The results showed that in the rhizosphere soil of Salvia miltiorrhiza Bunge discovered 10 strains beneficial microorganisms.And through the determination of reduplicative screening and its antibacterial spectrum,isolated 2 bacteriostatic strains：2-1 and 9-2.With the morphology and molecular biology identification，2-1 and 9-2 were bacillus subtilis and bacillus amyloliquefaciens.This lad a foundation for effective bio-control bacteria agent research and development.

参考文献/References:

[1] 卫生部药典委员会,中国药典（一部）[M].北京:化学工业出版社, 〖LL〗1995:63. [2] Du G H，Zhang J T.The general situation and progress of the modern 〖JP5〗research of red sage root ( Radix salviae miltiorrhizae)[J].Herald of Medicine, 2004，23:355-360. [3] 文家富,陈光华,王刚云,等.丹参根部病害发生与综合防治技术[J].中国植保导刊,2009,29(10):32-33. [4] 王刚云,文家富,郑小惠,等.陕西商洛丹参根部病害调查研究[J].陕西农业科学,2009,55(3):53-54. [5] 朱伟杰,王楠,郁雪平,等.生防菌 Pseudomonas fluorescens 2P24对甜瓜根围土壤微生物的影响[J].中国农业科学,2010,43(7):1389-1396. [6] 刘晓妹,刘文波,范秀利.芒果细菌性黑斑病生防菌的筛选及防效测定[J].中国生物防治,2006(10):94-97. [7] 李旭,马福海,王秀娟,等.大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素[J].生态学杂志,2012,31(6):1453-1460. [8] 汪雪静.草莓尖孢镰刀菌根腐病生防细菌的分离鉴定及抑菌作用的初步研究[D].北京:北京农学院,2012:14-16. [9] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社，2001：267-388. [10] 中国科学院微生物所细菌分类组.一般细菌常用鉴定方法[M].北京:科学出版社,1978. [11] 陈开森,余阳,孙爱娣,等.一株马脲片球菌的分离、鉴定及其部分16SrDNA基因序列测定[J].江西医学院学报,2009,49(2):41-43. [12] 刘海龙,李春杰,许艳丽.生防细菌的抑菌谱和对大豆根腐病的防治[J].大豆通报,2008(1):10-13.

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备注/Memo

第一作者简介：周莹（1987-），女，山东聊城人，硕士研究生，研究方向为生物制药研究。E-mail：hebe11183@yeah.net.责任作者：陈芳（1980-），女，山东惠民人，博士，副教授，现主要从事微生物制药等研究工作。E-mail：chenfang20045@163.com.基金项目：山东省自然科学基金资助项目（ZR2013CQ019）；聊城大学博士科研启动基金资助项目（3010）。

更新日期/Last Update: 2015-02-16