FENG Lidan,LI Jie,HE Jing,et al.Spraying BABA Regulate Reactive Oxygens Metabolism of Wolfberry Fruits to Improve Disease Resistance of Postharvest[J].Northern Horticulture,2023,(13):112-120.[doi:10.11937/bfyy.20224568]
喷施BABA调控枸杞活性氧代谢提高果实采后抗病能力
- Title:
- Spraying BABA Regulate Reactive Oxygens Metabolism of Wolfberry Fruits to Improve Disease Resistance of Postharvest
- Keywords:
- β-Aminobutyric acid; wolfberry; disease resistance; active oxygen; antioxidant
- 文献标志码:
- A
- 摘要:
- 以六年生枸杞‘宁杞7号’植株为试材,采用叶面喷施β氨基丁酸(BABA)的方法,研究了BABA对枸杞果实失重率、自然发病率、MDA含量、主要活性氧、抗氧化酶和抗氧化物质的影响,以期为增强枸杞鲜果采后抗病能力,减少果实的腐烂,延长保鲜时间提供参考依据。结果表明:采前叶面喷施1.0 mg?m-1 BABA诱导21 d后,可明显降低采后枸杞果实的失重率和自然腐烂率;抑制果实中超氧阴离子(O?2-)和过氧化氢(H2O2)的产生,显著提升超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活性;提升抗坏血酸-谷胱甘肽(AsA-GSH)循环中抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)的活性,提高抗坏血酸(AsA)和脱氧抗坏血酸还原酶(DHA)等非酶促抗氧化剂的含量,降低丙二醛(MDA)含量。在枸杞第1茬坐果后开始施用1 mg?mL-1 BABA,每7 d施用1次,可通过激活枸杞果实抗氧化酶活性,提升非酶促抗氧化剂的含量,将果实氧化程度控制在较低水平,有效减少果实的腐烂,延长保鲜时间。
- Abstract:
- The six years old wolfberry ‘Ningqi VII’ was used as the test material,and foliar spraying the β-Aminobutyric acid (BABA).The weight loss rate,natural incidence rate,MDA content,main active oxygen species,antioxidant enzymes and antioxidant substances effect by BABA in the wolfberry fruit were studied,in order to provide reference of wolfberry fresh fruit for enhancing the disease resistance after harvest,reducing fruit decay,and extending the preservation time.The results showed that foliar spraying 1.0 mg?mL-1 BABA before harvest,and induced 21 days could significantly reduce the weight loss rate and natural decay rate of postharvest wolfberry fruits;inhibited the production of O?2 -and H2O2 in fruits;increased the activities of antioxidant enzymes such as SOD,POD and CAT significantly;improved the activity of APX and GR,increased the content of non enzymatic antioxidants such as ASA and DHA in ASAGSH cycle;and reduced the content of MDA.Therefore,after the first fruit setting of wolfberry foliar sprayed 1.0 mg?mL-1 BABA for every 7 days,which could increase the content of non enzymatic antioxidants and activate the activity of antioxidant enzyme,control the degree of oxidation at a lower level,reduce the decay of fruit effectively,and extend the freshness preservation time.
参考文献/References:
[1]王益民,张珂,许飞华,等.不同品种枸杞子营养成分分析及评价[J].食品科学,2014,35(1):34-38.[2]章培军,邢雁霞,刘斌钰,等.枸杞多糖对实验性肝损伤保护作用的研究[J].中国药物与临床,2011,11(11):1286-1287.[3]FU Y W,PENG Y F,HUANG X D,et al.Lycium barbarum polysaccharidEglycoprotein preventative treatment ameliorates aversive[J].Neural Regeneration Research,2021,16(3):543-549.[4]ZHU Z,LUO W,SUN D W.Effects of liquid nitrogen quick freezing on polyphenol oxidase and peroxide activities,cell water states and epidermal microstructure of wolfberry[J].LWT,2020,120:108923.[5]向文娟,王孝雯,孙大文.水杨酸结合气调保鲜对宁夏枸杞贮藏品质的影响[J].食品科学,2022,43(9):215-222.[6]周宜洁,李新,马三梅,等.贮藏温度对鲜枸杞类胡萝卜素和氨基酸的影响及调控机制[J].科学通报,2022,67(Z1):385-395.[7]THEVENET D,PASTOR V,BACCELLI I,et al.The priming molecule betaaminobutyric acid is naturally present in plants and is induced by stress[J].New Phytologist,2017,213(2):552-559.[8]邱德文.植物免疫诱抗剂的研究进展与应用前景[J].中国农业科技导报,2014,16(1):39-45.[9]高静,王楠,刘阿萍,等.β氨基丁酸对吊竹梅的气孔运动影响机制初探[J].陕西农业科学,2019,65(4):71-73,82.[10]OORT A J,ANDEL O V.Aspects in chemotherapy[J].Mededel Opz Gent,1960,25(2):961-992.[11]PAPAVIZAS G,DAVEY C.Effect of sulfurcontaining amino compounds and related substances on Aphanomyces root rot of peas[J].Phytopathology,1963,53:109-115.[12]SILU D,PAJOT E,COHEN Y.Induction of resistance to downy mildew(Peronospora parasitica) in cauliflower by DLβ-amino n butanoic acid (BABA)[J].Plant Pathology,2002,51(1):97-102.[13]HAMIDUZZAMAN M M,JAKAB G,BARNAVON L,et al.Beta Aminobutyric acid induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling[J].Molecular Plant microbe Interactions:MPMI,2005,18(8):819-829.[14]LAZZARATO L,TREBBI G,PAGNUCCO C,et al.Exogenous spermidine,arsenic and betaaminobutyric acid modulate tobacco resistance to tobacco mosaic virus,and affect local and systemic glucosylsalicylic acid levels and arginine decarboxylase gene expression in tobacco leaves[J].J Plant Physiol,2009,166(1):90-100.[15]WALZ A,SIMON O.β-Aminobutyric acidinduced resistance in cucumber against biotrophic and necrotrophic pathogens[J].Journal of Phytopathology,2009,157(6):356-361.[16]王海霞,余小玲,戚烨通,等.超量表达响应β-氨基丁酸诱导的 StWRKY8基因提高马铃薯晚疫病抗性[J].农业生物技术学报,2018,26(7):1107-1115.[17]LI R,SHEN J,SHEN L.Nitric oxide plays an important role in betaaminobutyric acidinduced resistance to Botrytis cinerea in tomato plants[J].Plant Pathology Journal,2020,36(2):121-132.[18]王静,王海霞,田振东.β氨基丁酸诱导马铃薯对晚疫病的抗性组织化学及信号传导途径分析[J].中国农业科学,2014,47(13):2571-2579.[19]范存斐,毕阳,王云飞,等.水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响[J].中国农业科学,2012,45(3):584-589.[20]REN Y,WANG Y,BI Y,et al.Postharvest BTH treatment induced disease resistance and enhanced reactive oxygen species metabolism in muskmelon(Cucumis melo L.) fruit[J].European Food Research and Technology,2012,234(6):963-971.[21]YUAN L,BI Y,GE Y,et al.Postharvest hot water dipping reduces decay by inducing disease resistance and maintaining firmness in muskmelon(Cucumis melo L.) fruit[J].Scientia Horticulturae,2013,161:101-110.[22]KAMPFENKEL K,VAN MONTAGU M,INZE D.Extraction and determination of ascorbate and dehydroascorbate from plant tissue[J].Analytical Biochemistry,1995,225(1):165-167.[23]CAO S,YANG Z,CAI Y,et al.Fatty acid composition and antioxidant system in relation to susceptibility of loquat fruit to chilling injury[J].Food Chemistry,2011,127(4):1777-1783.[24]耿艺.低/高湿小白菜的降压调控真空预冷研究[D].广州:华南理工大学,2020.[25]MITTLER R.ROS are good[J].Trends Plant Sci,2017,22(1):11-19.[26]WASZCZAK C,CARMODY M,KANGASJARVI J.Reactive oxygen species in plant signaling[J].Annu Rev Plant Biol,2018,69:209-236.[27]APEL K,HIRT H.Reactive oxygen species:Metabolism,oxidative stress,and signal transduction[J].Annu Rev Plant Biol,2004,55:373-399.[28]APOSTOL L,HEINSTEIN P F,PHILIP S.Low rapid stimulation of an oxidative burst during eliciation of cultured plant cells:Role in defense and signal transduction[J].Plant Physiology,1989,90(1):109-116.[29]井一诺,王雷.β-氨基丁酸抑制鸭梨果实采后青霉病的机理研究[J].食品研究与开发,2017,38(12):169-174.[30]谭卫萍,庞学群,张昭其,等.BABA诱导香蕉果实抗病性与贮藏期活性氧积累的关系[J].中国农业科学,2014,47(16):3290-3299.[31]汪跃华,徐兰英,庞学群,等.BABA处理提高采后砂糖桔对青霉菌的抗病性[J].食品科技,2011,36(3):34-37,41.[32]CONKLIN P.Environmental stress sensitivity of an ascorbic acisdeficient Arabidopsis mutant[J].Proc Natl Acad Sci USA,1996,93:9970-9974.[33]CREISSEN,FRYER,KULAR,et al.Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress[J].Plant Cell,1999,11(7):1277-1292.[34]王懿,侯媛媛,马钰晴,等.甘氨酸甜菜碱处理对桃果实冷害及抗坏血酸谷胱甘肽循环代谢的影响[J].食品科学,2021,42(13):158-165.[35]陈偲.BABA处理控制采后桃果实腐烂的作用及其机理研究[D].南京:南京农业大学,2019.[36]WANG K,LI C,LEI C,et al.beta aminobutyric acid induces priming defence against Botrytis cinerea in grapefruit by reducing intercellular redox status that modifies posttranslation of VvNPR1 and its interaction with VvTGA1[J].Plant Physiology and Biochemistry,2020,156:552-565.[37]WANG K,WU D,BO Z,et al.Regulation of redox status contributes to priming defense againstBotrytis cinerea in grape berries treated with beta aminobutyric acid[J].Scientia Horticulturae,2019,244:352-364.[38]LI C,WANG K,ZHENG Y.Redox status regulates subcelluar localization of PpTGA1 associated with a BABA induced priming defence against Rhizopus rot in peach fruit[J].Molecular Biology Reports,2020,47(9):6657-6668.[39]汪开拓,雷长毅,黎春红,等.BABA处理对草莓采后灰霉病的控制及其转录组学分析[J].果树学报,2020,37(11):1744-1757.
相似文献/References:
[1]武小靖,杨 晴,张国君,等.土壤含盐量对枸杞生理生化指标的影响[J].北方园艺,2014,38(12):137.
WU Xiao-jing,YANG Qing,ZHANG Guo-jun,et al. Effect of Salinity of Soil on Physio-biochemistry Indexes of Lycium barbarum[J].Northern Horticulture,2014,38(13):137.
[2]王丽娟,赵 辉,王彦才,等.枸杞酸性转化酶基因的克隆及组织表达分析[J].北方园艺,2014,38(01):86.
WANG Li-juan,ZHAO Hui,WANG Yan-cai,et al.Cloning and Tissues Expression Analysis of Soluble Acid Invertase Gene from Lycium barbarum.L.[J].Northern Horticulture,2014,38(13):86.
[3]王亚军,安巍,罗青,等.丛枝菌根真菌菌剂扩繁及菌根化枸杞育苗技术研究[J].北方园艺,2014,38(05):139.
Research on Enlargment Reproducing of AMF Microbial Inoculum and Growing Seedling Technology of Wolfberry With Mycorrhization.WANG Ya-jun1,2,AN Wei1,LUO Qing1,MA Ping3,SHI Zhi-gang1,ZHAO Jian-hua1[J].Northern Horticulture,2014,38(13):139.
[4]樊光辉.“宁杞1号”枸杞辐射诱变育种初报[J].北方园艺,2014,38(02):149.
FAN Guang-hui.Preliminary Report on ‘Ningqi No. 1’ Lycium barbarum Radiation Mutation Breeding[J].Northern Horticulture,2014,38(13):149.
[5]魏雯雯,班兆军,冯建华,等.热处理对‘宁杞4号’鲜食枸杞果实冷藏品质的影响[J].北方园艺,2013,37(10):124.
WEI Wen-wen,BAN Zhao-jun,FENG Jian-hua,et al.Effects of Heat Treatment on Quality of Lycium barbarum Druit During Storage[J].Northern Horticulture,2013,37(13):124.
[6]刘长江,匡 明.枸杞钙果复合运动饮料的研制[J].北方园艺,2013,37(17):129.
LIU Chang-jiang,KUANG Ming.Manufacture of Medlar-calcium Fruit Compound Health Beverage[J].Northern Horticulture,2013,37(13):129.
[7]王忠忠,马茜,慕金凤,等.宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究[J].北方园艺,2013,37(11):152.
WANG Zhong-zhong,MA Qian,MU Jin-feng,et al.Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.[J].Northern Horticulture,2013,37(13):152.
[8]鲍瑞,周筠,康建宏,等.不同有机肥对枸杞活性成分的影响[J].北方园艺,2013,37(05):174.
BAO Rui,ZHOU Yun,KANG Jian-hong,et al.Effect of Different Organic Fertilizer Treatments on Active Ingredient of Lycium barbarum L.[J].Northern Horticulture,2013,37(13):174.
[9]郑国琴.,柳金凤.宁夏枸杞叶片组培快繁技术研究[J].北方园艺,2012,36(01):122.
ZHENG Guo-qin.,LIU Jin-feng.Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.[J].Northern Horticulture,2012,36(13):122.
[10]何 军,李晓莺,焦恩宁,等.三个枸杞品种花粉直感效应研究[J].北方园艺,2013,37(09):178.
HE Jun,LI Xiao-ying,JIAO En-ning,et al.Study on Pollen Xenia Effect of Three Wolfberry Cultivars[J].Northern Horticulture,2013,37(13):178.
备注/Memo
第一作者简介:冯丽丹(1981-),女,硕士,讲师,现主要从事食品科学等研究工作。Email:yanzedan1982@163.com.责任作者:李捷(1981-),男,博士,副教授,现主要从事经济林栽培等研究工作。Email: lj81658@gsau.edu.cn.基金项目:甘肃省高校创新基金资助项目(2020A055);甘肃省特色优势农产品评价资助项目(TYNPZ2022-13)。收稿日期:2022-11-09