CAO Deyou,WANG Yuxia,LI Chunjun,et al.Genetic Identification and Agronomic Traits of Monocaryon Hybrid Strain of Auricularia auricula Judae[J].Northern Horticulture,2022,(20):119-124.[doi:10.11937/bfyy.20220928]
黑木耳单核杂交菌株鉴定与农艺性状评价
- Title:
- Genetic Identification and Agronomic Traits of Monocaryon Hybrid Strain of Auricularia auricula Judae
- Keywords:
- Auricularia auricula Judae; protoplast monokaryon hybridization; agronomic character; grey relational
- 文献标志码:
- A
- 摘要:
- 以完成了酯酶同工酶酶谱鉴定的黑木耳亲本菌株、单核菌株和栽培筛选的“Z5”杂交菌株为试验材料，采用RAPD分子图谱比较和栽培农艺性状灰色关联度分析方法，研究了单核杂交菌株与亲本菌株的图谱差别、遗传关系，并对杂交菌株栽培性状进行比较，以期获得适合市场需求的性状改良的新菌株。结果表明：RAPD分子图谱显示“Z5”菌株缺失亲本及杂交单核的部分图谱条带，也产生了新条带。聚类分析显示“Z5”菌株与2个亲本相似度仅为54%，遗传距离较远。根据给出的参考品种性状值比较了“Z5”菌株和2个亲本栽培农艺性状和灰色关联度，“Z5”菌株农艺性状可满足现阶段市场要求，其等权关联度和加权关联度排在了2个亲本之前。
- Abstract:
- Taking the parent strain of Auricularia auricula, the mononuclear strain and the ‘Z5’ hybrid strain,which had been identified by esterase isozyme zymogram,as the experimental materials,the map difference and genetic relationship between the mononuclear hybrid strain and the parent strain were studied by RAPD molecular map comparison and the gray correlation analysis of cultural and agronomic characters,and the cultural characters of the hybrid strains were compared to obtain the new strains with improved characters suitable for the market demand.The results showed that RAPD molecular map showed that ‘Z5’ strain lacked some map bands of parents and hybrid mononuclear,and also produced new bands.Cluster analysis showed that the similarity between ‘Z5’ strain and two parents was only 54%,and the genetic distance was far.The agronomic characters and grey correlation degree of ‘Z5’ strain and two parents were compared according to the given reference variety character values.The agronomy of ‘Z5’ strain could meet the current market requirements,and its equal weight correlation degree and weighted correlation degree rank ahead of the two parents.
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备注/Memo
第一作者简介：曹德友（1973-），男，硕士，现主要从事食用菌研究现状与发展等研究工作。E-mail:13766800999@163.com.责任作者：韩增华（1968-），女，本科，研究员，硕士生导师，现主要从事食药用菌遗传育种等研究工作。E-mail:hanzenghua77@126.com.基金项目：黑龙江省院所基本应用技术研究专项资助项目（ZNJZ2020SW01）;黑龙江省科学院中试与熟化项目（ZSSH202005）。收稿日期：2022-03-14