GUO Chengjin,CHEN Hang,WANG Xigang,et al.Effects of Compound Microbial Agent Added to Seedling Substrate on Tomato Growth and Root Rot Occurrence[J].Northern Horticulture,2022,(16):52-57.[doi:10.11937/bfyy.20220698]
育苗基质添加复配微生物菌剂对番茄生长及根腐病发生的影响
- Title:
- Effects of Compound Microbial Agent Added to Seedling Substrate on Tomato Growth and Root Rot Occurrence
- Keywords:
- Clonostachys rosea; Bacillus subtilis; seedling substrate; synergistic effect; tomato root rot
- 文献标志码:
- A
- 摘要:
- 以“博远2号”番茄为试材，采用育苗基质添加粉红螺旋聚孢霉菌剂和枯草芽孢杆菌菌剂单独或联合使用的方法，研究了不同处理对设施番茄生长、产量、品质及其根腐病防治效果的影响，以期为制定宁夏设施番茄土传病害生物防治技术提供参考依据。结果表明：育苗基质中添加粉红螺旋聚孢霉菌剂和枯草芽孢杆菌菌剂单独或联合使用均能促进番茄种苗生长，降低根腐病发病率，联合使用处理效果最好。联合使用处理番茄幼苗出苗率和株高分别为9722%和667 cm，均明显高于对照；移栽前联合使用处理的番茄株高、根长、鲜质量和干质量分别较对照提高了702%、2153%、923%和3333%；联合使用处理的番茄根际土壤中真菌和芽孢杆菌数量分别是对照的10倍和100倍；定植后联合使用处理的番茄株高和茎粗较对照显著提高了896%和2820%，根腐病较对照显著降低了5140%；联合使用处理的番茄单果质量、单株产量和667 m2产量分别较对照显著提高了681%、1397%和1546%，番茄可溶性糖含量和糖酸比分别较对照显著提高了1708%和4638%。综上所述，1 kg基质中添加01 g粉红螺旋聚孢霉菌剂和2 g枯草芽孢杆菌菌剂对番茄具有较好的防病促生作用。
- Abstract:
- Taking tomato cultivar ‘Boyuan No2’ as test material，seedling substrate add Clonostachys rosea and Bacillus subtilis alone or in combination method were used to study the effects of different treatments to tomato growth，yield，quality and root rot，in order to provide reference for Ningxia greenhouse tomato soil-borne disease biological control technology.The results showed that addition of Clonostachys rosea and Bacillus subtilis alone or in combination in seedling substrate could promote the growth of tomato seedlings and reduced the incidence of root rot，and the combined treatment was the best.The emergence rate and shoot height of tomato treated with the combined treatment were 9722% and 667 cm，respectively，significantly higher than CK.The shoot height，root length，fresh biomass and dry biomass of tomato treated with the combined treatment were increased by 702%，2153%，923% and 3333%，respectively，before transplanting.The numbers of rhizosphere soil fungi and Bacillus sp.in the combined treatment were 10 times and 100 times higher than CK，respectively.After transplanting，the shoot height and stem diameter of tomato treated with the combined treatment significantly increased by 896% and 2820% respectively compared with CK，while the root rot of tomato treated with the combined treatment was significantly reduced by 5140% compared with CK.The weight of single fruit，yield per plant and yield per 667 m2 of tomato treated with the combined treatment were 681%，1397% and 1546% significantly higher than CK，respectively.The soluble sugar content and sugar-acid ratio of tomato treated with the combined treatment were significantly increased by 1708% and 4638% respectively compared with CK.In conclusion，the addition of 01 g of Clonostachys rosea agent and 2 g of Bacillus subtilis agent per kilogram of seedling substrate had better disease control and tomato growth promotion effect.Keywords:Clonostachys rosea；B
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备注/Memo
第一作者简介：郭成瑾（1978-），女，博士，副研究员，现主要从事作物病害综合防治等研究工作。E-mail：chj.guo@163.com.责任作者：沈瑞清（1964-），男，博士，研究员，现主要从事植物病害防治等研究工作。E-mail：srqzh@sina.com.基金项目：宁夏回族自治区重点研发计划重大（重点）资助项目（2019BFF02006）；宁夏回族自治区自然科学基金资助项目（2020AAC03316）；宁夏回族自治区青年拔尖人才培养工程资助项目（宁人社函[2017]787号）。收稿日期：2022-02-28