CAO Zijian,WANG Shouxian,ZHENG Suyue,et al.Identification of Paecilomyces variotii on Contaminated Artificial Bed-logs of Lentinula edodes and Their Interactions[J].Northern Horticulture,2022,(14):116-125.[doi:10.11937/bfyy.20220036]
香菇菌棒污染菌宛氏拟青霉的鉴定及其与香菇菌丝的相互作用
- Title:
- Identification of Paecilomyces variotii on Contaminated Artificial Bed-logs of Lentinula edodes and Their Interactions
- Keywords:
- Lentinula edodes; Paecilomyces variotii; contaminating fungus; biological characteristics; interaction
- 文献标志码:
- A
- 摘要:
- 以河北省平泉县发现的香菇污染菌棒为试材，采用组织分离法结合形态学特征、多基因序列分析方法，研究了该污染菌的生物学特性及其与生产中普遍种植的4种香菇菌丝的相互作用，以期为香菇菌棒拟青霉的防治提供参考依据。结果表明：鉴定污染菌为宛氏拟青霉（Paecilomyces variotii），宛氏拟青霉嗜高温，致死温度高达89 ℃，其菌丝体生长的最佳条件为温度35 ℃，pH 6，葡萄糖作为碳源，氯化铵作为氮源，光照对菌丝生长有一定的促进作用；宛氏拟青霉对不同香菇栽培菌棒的污染能力存在差异，其可通过产生次生代谢产物、竞争空间和养分等方式抑制香菇菌丝的生长。在香菇生产过程中需严格控制温度条件，同时加强通风和环境卫生管理有助于预防宛氏拟青霉的发生和为害。该研究是宛氏拟青霉污染香菇栽培菌棒的首次报道。
- Abstract:
- The contaminated artificial bed-logs of Lentinus edodes found in Pingquan，Hebei Province were used as the test materials，the method of tissue isolation combined with morphological characteristics and multiple gene sequences analysis were used.The biological characteristics of contaminating bacteria and its interaction with the hyphae of four L.edodes strains widely planted in production were further studied，in order to provide reference basis for the control of contaminated artificial bed-logs of Lentinus edodes caused by P.variotii.The results showed that the contaminated bacterium was identified as Paecilomyces variotii,P.variotii was thermophilic，and the lethal temperature was as high as 89 ℃.The optimum conditions for the growth of its hyphae were as follows,temperature 35 ℃，pH 6，glucose as carbon source and ammonium chloride as nitrogen source.Light promoted the hyphae growth of P.variotii to a certain extent.Paecilomyces variotii showed varied polluting capacity to the artificial bed-logs from different strains of L.edodes.It could inhibit the hyphae growth of L.edodes by producing secondary metabolites，competing space and nutrients.It was necessary to strictly control the temperature conditions，strengthen ventilation and environmental sanitation management in the process of production，which was helpful to prevent the occurrence and harm of P.variotii.This was the first report of P.variotii contaminated the artificial bed-logs on the cultivation of L.edodes.
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备注/Memo
第一作者简介：曹子健（1997-），男，硕士研究生，研究方向为食用菌病害防控。E-mail：18821710624@163.com.责任作者：秦文韬（1988-），女，博士，助理研究员，现主要从事食用菌病害诊断与防控技术等研究工作。E-mail：qinwentao@ipepbaafs.cn.基金项目：北京市农林科学院创新能力建设专项资助项目（KJCX20220415）；北京市食用菌创新团队资助项目（BAIC05-2022）；国家食用菌产业技术体系资助项目（CARS-20）。收稿日期：2022-01-05