[1]龙思宇,李萌,邓亚婷,等.ALA对干旱胁迫下绣球生理特性的影响[J].北方园艺,2022,(10):63-69.[doi:10.11937/bfyy.20214364]
　LONG Siyu,LI Meng,DENG Yating,et al.Effects of 5-Aminolevulinic Acid (ALA) on Physiological Characteristics of Hydrangea Under Drought Stress[J].Northern Horticulture,2022,(10):63-69.[doi:10.11937/bfyy.20214364]
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ALA对干旱胁迫下绣球生理特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年10 页码: 63-69 栏目: 出版日期: 2022-05-30
Title:
Effects of 5-Aminolevulinic Acid (ALA) on Physiological Characteristics of Hydrangea Under Drought Stress
作者:
龙思宇1; 李萌2; 邓亚婷2; 毛容婕2; 谭子祎2; 曹受金1
（1.中南林业科技大学 林学院，湖南 长沙 410004；2.中南林业科技大学 生命科学与技术学院,湖南 长沙 410004）
Author(s):
LONG Siyu1; LI Meng2; DENG Yating2; MAO Rongjie2; TAN Ziyi2; CAO Shoujin1
(1 College of Forestry，Central South University of Forestry and Technology，Changsha，Hunan 410004;2 College of Life Science and Technology，Central South University of Forestry and Technology，Changsha，Hunan 410004)
关键词:
绣球; 干旱胁迫; ALA; 生理特性
Keywords:
Hydrangea macrophylla; drought stress; ALA; physiological characteristics
DOI:
10.11937/bfyy.20214364
文献标志码:
A
摘要:
以绣球品种‘无尽夏’盆栽为试材，采用盆栽控水和叶面喷施(ALA)法，研究了不同质量浓度（0、25、50、75、100 mg?L-1）的ALA溶液对干旱胁迫下绣球叶片的可溶性糖含量、可溶性蛋白质含量、游离脯氨酸（Pro）含量、抗氧化酶（超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX））活性、丙二醛（MDA）含量、超氧阴离子（O?〖TX--*9〗2）产生速率的影响，以期为研究外源ALA增强植物在干旱胁迫下的抗旱能力提供参考依据，为提高绣球对干旱胁迫环境的生理适应性提供可行性方案。结果表明：干旱胁迫下绣球叶片表型失绿、渗透调节物质大量积累、抗氧化酶活性增强，植物生长受到抑制；适宜浓度的ALA溶液可以提高干旱胁迫下绣球渗透调节物质含量、抗氧化酶活性，缓解活性氧伤害，降低干旱胁迫对细胞质膜抗氧化程度，外源ALA可以有效缓解干旱胁迫对绣球的伤害，增强绣球对干旱胁迫的适应能力。
Abstract:
Hydrangea macrophylla variety ‘Endless Summer’ potted plants were used as materials,the method of potted water control and foliar spraying ALA were measured to study the effects of different concentrations (0，25，50，75，100 mg?L-1)ALA on the soluble sugar content，soluble protein content，free proline(Pro) content，antioxidant enzyme (SOD，POD，CAT，APX)activities of H.macrophylla leaves，as well as malondialdehyde (MDA) content and O?2 production rate,in order to provide reference for the study of exogenous ALA to enhance the drought resistance of plants under drought stress，and to provide a feasible scheme for improving the physiological adaptability of H.macrophylla to drought stress environment.The results showed that under drought stress，H.macrophylla leaves phenotype was chlorosis，osmotic adjustment substances accumulated in a large amount，antioxidant enzyme activity was enhanced，and plant growth was inhibited.Exogenous spraying of ALA solution at a suitable concentration could significantly increase the osmotic adjustment substance content and antioxidant enzyme activity of H.macrophylla under drought stress，alleviated the damage of active oxygen，and reduced the antioxidant degree of drought stress on cell plasma membrane.Exogenous ALA could effectively alleviate the damage of drought stress to H.macrophylla and enhance the adaptability of H.macrophylla to drought stress.

参考文献/References:

[1]任洁.八仙花的应用及研究进展[J].绿色科技,2018(13):165-167.[2]WU Y，LIAO W，DAWUDA M M，et al.5-aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants：A review[J].Plant Growth Regulation，2019，87(2):357-374[3]牛奎举.外源5-氨基乙酰丙酸对干旱胁迫下草地早熟禾光合作用的调控机制[D].兰州:甘肃农业大学，2018.[4]王颖,孔德浩,陈佰鸿,等.外源ALA对干旱胁迫下山定子叶片叶绿素荧光特性及抗性生理指标的影响[J].西北植物学报,2018,38(5):902-911.[5]张春平,何平,袁凤刚,等.外源5-氨基乙酰丙酸对干旱胁迫下甘草种子萌发及幼苗生理特性的影响[J].西北植物学报,2011,31(8):1603-1610.[6]章毅,韦孟琪,孙欧文,等.不同绣球品种对干旱胁迫的生理响应及抗旱机制研究[J].西北林学院学报,2018,33(1):90-97.[7]HSIAO T C.Plant responses to water stress[J].Annual Review of Plant Physiology,1973(24):519-570.[8]孙欧文,杨倩倩,章毅,等.四个绣球品种对高温干旱复合胁迫的生理响应机制[J].植物生理学报,2019,55(10):1531-1544.[9]王学奎，黄见良.植物生理生化实验原理与技术[M].北京:高等教育出版社,2015.[10]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[11]李忠光,龚明.植物中超氧阴离子自由基测定方法的改进[J].云南植物研究,2005(2):211-216.[12]李柯,周庄煜,李四菊,等.荆芥的生长、渗透调节和抗氧化能力对干旱胁迫的响应[J].草业学报,2020,29(5):150-158.[13]薄伟.3种景天属植物对持续干旱的生理响应及抗旱性综合评价[J/OL].分子植物育种.(2017-04-28)[2021-10-29].https://biopublisher.cn/index.php/mpbopa/article/view/6731.[14]BOOGAR A R，SALEHI H,JOWKAR A.Exogenous nitric oxide alleviates oxidative damage in turfgrasses under drought stress[J].South African Journal of Botary,2014,92:78-82.[15]ZHANG C，SHI S，WANG B，et al.Physiological and biochemical changes in different drought-tolerant alfalfa (Medicago sativa L.) varieties under peg-induced drought stress[J].Acta Physiologiae Plantarum，2018，40:25.[16]张治平,戴海博,耿园.叶面喷施不同浓度ALA对黄瓜幼苗抗氧化酶系统的影响[J].长江蔬菜,2017(20):88-92.[17]李阳阳.叶面喷施5-氨基乙酰丙酸对葡萄光合作用及蔗糖转化酶基因表达的影响[D].杨凌:西北农林科技大学,2018.[18]焦健,李朝周,黄高宝.钴对干旱胁迫下大豆幼苗叶片的保护作用及其机理[J].应用生态学报,2006(5):796-800.[19]王发展,金伊楠,李子玮,等.干旱胁迫下外源ALA对烤烟幼苗光合特性和抗氧化能力的影响[J].中国烟草科学,2020,41(1):22-29.[20]刘福顺,陈媛媛,李宗谕,等.ALA对盐胁迫下苦参种子萌发及幼苗生理特性的影响[J/OL].分子植物育种.(2021-05-31)[2021-10-29].http://kns.cnki.net/kcms/detail/46.1068.S.20210531.0945.002.html.

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备注/Memo

第一作者简介：龙思宇（1998-），女，硕士研究生，研究方向为园林植物与观赏园艺。E-mail:18597848985@163.com.责任作者：曹受金（1972-），男，博士，教授，现主要从事园林植物与观赏园艺等研究工作。E-mail:932143364@qq.com.基金项目：湖南省教育厅科学研究重点资助项目(19A524)。收稿日期：2021-10-29

更新日期/Last Update: 2022-07-04