FANG Zhirong,QING Yuan,HE Xiaolan,et al.Identification and Biological Characters of Two Wild Lentinula Strains[J].Northern Horticulture,2022,(06):107-113.[doi:10.11937/bfyy.20213873]
两株野生微香菇属（Lentinula）真菌的鉴定和生物学特性
- Title:
- Identification and Biological Characters of Two Wild Lentinula Strains
- 文献标志码:
- A
- 摘要:
- 以四川省普格县野生微香菇属真菌（Lentinula）子实体为试材，采用组织分离的方法得到菌株HG-2、BG-1，并对其进行分子鉴定；同时研究了不同碳源、氮源、温度以及pH对HG-2、BG-1菌株菌丝生长的影响，以期为野生食用菌资源的开发利用提供参考依据。结果表明： 2个野生菌株均为微香菇属（Lentinula edodes）真菌。HG-2、BG-1菌株菌丝体生长的最佳碳源为玉米粉，最佳氮源为酵母膏，最适培养温度为25 ℃，最适pH为6.0。
- Abstract:
- Taking the fruiting bodies of wild Lentinula fungus collected from the mountain in Puge county,Sichuan Province as the test material,strains HG-2 and BG-1 were obtained by tissue isolation.Strains HG-2 and BG-1 were identified by DNA sequences.The effects of different carbon sources,nitrogen sources,temperature and pH on the growth of HG-2 and BG-1 strains were studied,in order to provide a reference for the utilization of wild Lentinula resources.The results showed that the two wild strains were Lentinula edodes.The optimum carbon source for the mycelial growth of HG-2 and BG-1 strains was corn meal,the optimum nitrogen source was yeast extract; the optimal cultivation temperature was 25 ℃,and the optimal pH was 6.0.
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备注/Memo
第一作者简介：方志荣(1981-)，女，博士，讲师，现主要从事固体废物处理、处置科研与教学等工作。E-mail:172580110@qq.com.基金项目：凉山彝族自治州科学技术局资助项目（17NYCX0023)；攀西特色作物研究与利用四川省重点实验室资助项目（XNFZ2109）。收稿日期：2021-09-17