WANG Lei,YAN Xingfu,TANG Zhanhui.Effects of Three Species of Arbuscular Mycorrhizal Fungi on the Growth of Lychnis cognata Maxim[J].Northern Horticulture,2021,(20):77-83.[doi:10.11937/bfyy.20211282]
三种丛枝菌根真菌对浅裂剪秋萝生长的影响
- Title:
- Effects of Three Species of Arbuscular Mycorrhizal Fungi on the Growth of Lychnis cognata Maxim
- Keywords:
- Lychnis cognata Maxim; arbuscular mycorrhizal fungi(AMF); biomass; chlorophyll content; root characteristics
- 文献标志码:
- A
- 摘要:
- 以石竹科植物浅裂剪秋萝(Lychnis cognata Maxim)为试材，在温室盆栽条件下分别接种摩西斗管囊霉（Funneliformis mosseae，GM）、地表球囊霉（Glomus versiforme，GV）和根内根孢囊霉（Rhizophagus intraradices，GI），研究了3种丛枝菌根真菌(arbuscular mycorrhizal fungi，AMF)对浅裂剪秋萝形态和生长指标及根系侵染情况的影响，以期为在人工条件下利用AMF促进野生花卉植物栽培和资源开发提供参考依据。结果表明：3种AMF均能与浅裂剪秋萝形成菌根共生结构，GV、GI和GM的根系侵染率分别为35.71%、23.00%和17.50%；但浅裂剪秋萝对3种AMF的菌根依赖性无显著性差异；与对照相比接种3种AMF并未使浅裂剪秋萝株高、地上生物量、地下生物量及总生物量和根冠比产生显著差异；仅GI显著增加了浅裂剪秋萝分枝数；此外3种AMF对浅裂剪秋萝叶绿素含量与根系活力和根系结构未产生显著影响。综上所述，在该试验条件下，虽然AMF能够与浅裂剪秋萝形成菌根缠绕结构，但其因植物自身因素与培养条件等并未对浅裂剪秋萝生长产生明显影响，该结果可为进一步探索非菌根石竹科植物与AMF的相互作用提供参考依据。
- Abstract:
- Lychnis cognata Maxim was used as the experimental material,and the L.cognata was inoculated by using three arbuscular mycorrhizal fungi (AMF) species including Funneliformis mosseae (GM),Glomus versiforme (GV) and Rhizophagus intraradices (GI) under greenhouse conditions.The effects of inoculation by the three AMF species on morphology,growth performance and root infection of L.cognata were studied,in order to provide a reference for using AMF to promote the cultivation and resource development of wild ornamental plants under artificial conditions.The results showed that all the three AMFs could form mycorrhizal symbiotic structure with L.cognata.The root infection rates of GV,GI and GM were 35.71%,23.00% and 17.50%,respectively.However,there was no significant difference in the mycorrhizal dependence among the three species of AMF.There were no significant differences in plant height,aboveground biomass,underground biomass,total biomass and root-shoot ratio of L.cognata inoculated with AMF compared with the control.The branching number of L.cognata significantly increased when inoculated by GI.In addition,three species of AMF had no significant effects on chlorophyll content,root activity and root structure of L.cognate.In conclusion,although AMF can form mycorrhizal entangling structure with L.cognata under the experimental conditions,it had no significant effect on the growth of L.cognata.The results could provide a reference for further exploration of the interaction between non-mycorrhizal plants and AMF.
参考文献/References:
[1]BERRUTI A,LUMINI E,BALESTRINI R,et al.Arbuscular mycorrhizal fungi as natural biofertilizers：Let′s benefit from past successes[J].Frontiers in Microbiology,2016,6(426)：1559.[2]MORRIS E K,MORRIS D J P,VOGT S,et al.Visualizing the dynamics of soil aggregation as affected by arbuscular mycorrhizal fungi[J].ISME Journal,2019（13）：1639-1646.[3]AGUS C,PRIMANANDA E,FARIDAH E,et al.Role of arbuscular mycorrhizal fungi and Pongamia pinnata for revegetation of tropical open-pit coal mining soils[J].International Journal of Environmental Science and Technology,2019,16(7)：3365-3374.[4]ZHANG H,XU N,LI X,et al.Arbuscular mycorrhizal fungi (Glomus mosseae) improves growth,photosynthesis and protects photosystemII in leaves of Lolium perenne L.in cadmium contaminated soil[J].Frontiers in Plant Science,2018(9)：1156.[5]ABDEL-FATTAH G M,ASRAR A A,AL-AMRI S M,et al.Influence of arbuscular mycorrhiza and phosphorus fertilization on the gas exchange,growth and phosphatase activity of soybean(Glycine max L.) plants[J].Photosynthetica,2014,52(4)：581-588.[6]HIIESALU I,PARTEL M,DAVISON J,et al.Species richness of arbuscular mycorrhizal fungi：Associations with grassland plant richness and biomass[J].New Phytologist,2014,203：233-244.[7]刘丽丽,刘仁道,黄仁华.丛枝菌根真菌(AMF)对红阳猕猴桃叶片富硒能力及光合特性的影响[J].食品工业科技,2014,35(10):234-237,242.[8]LI W B,LU W Q,XIE J W,et al.Effects of arbuscular mycorrhizal fungi on the growth and cut flower physiology of Tulipa gesneriana[J].Mycosystema,2018,37(4)：456-465.[9]SAYYAHFAR M,MIRSHEKARI B,YARNIA M,et al.Effect of mycorrhizal inoculation and methanol spraying on some photosynthetic characteristics and yield in wheat cultivars under endseason drought stress[J].Applied Ecologyand Environmental Research,2018,16(4)：3783-3800.[10]PAVITHRA D,YAPA N.Arbuscular mycorrhizal fungi inoculation enhances drought stress tolerance of plants[J].Groundwater for Sustainable Development,2018(7)：490-494.[11]ZHANG Z F,ZHANG J C,HUANG Y Q.Effects of arbuscular mycorrhizal fungi on the drought tolerance of Cyclobalanopsis glauca seedlings under greenhouse conditions[J].New Phytologist,2014,45：545-556.[12]BASLAM M,ESTEBAN R,GARCIA-PLAZAOLA J I,et al.Effectiveness of arbuscular mycorrhizal fungi(AMF) for inducing the accumulation of major carotenoids,chlorophylls and tocopherol in green and red leaf lettuces[J].Applied Microbiologyand Biotechnology,2013,97(7)：3119-3128.[13]MOHAN J E,COWDEN C C,BAAS P,et al.Mycorrhizal fungi mediation of terrestrial ecosystem responses to global change：Mini-review[J].Fungal Ecology,2014(10)：3-19.[14]BRUNDRETT M C.Mycorrhizal associations and other means of nutrition of vascular plants：Understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis[J].Plant and Soil,2009,320：37-77.[15]初亚男,张海波,秦泽峰,等.AM 真菌与非菌根植物的相互作用关系[J].应用生态学报,2018,29(1)：321-326.[16]HIRREL M C,MEHRAVARAN H,GERDEMANN J W.Vesicular-arbuscular mycorrhizae in the chenopodiaceae and cruciferae：Do they occur?〖KG-*3〗[J].Canadian Journal of Botany-revue Canadienne de Botanique,2011,56：2813-2817.[17]包宇,乔娅楠,闫兴富,等.浅裂剪秋萝和丝瓣剪秋萝传粉特性的初步研究[J].植物学报,2018,53(5):634-642.[18]岳莉然,程贝贝.盐碱胁迫及干旱胁迫对浅裂剪秋萝种子萌发的影响[J].北方园艺,2019(10)：86-93.[19]ZHU X Q,TANG M,ZHANG H Q.Arbuscular mycorrhizal fungi enhanced the growth,photosynthesis,and calorific value of black locust under salt stress[J].Photosynthetica,2017,55(2)：378-385.[20]BIERMANN B,LINDERMAN R G.Quantifying vercula arbuscular mycorrhizas：A proposed method towards standardization[J].New Phytologist,1981,87：63-67.[21]弓明钦,王凤珍,陈羽,等.西南桦对菌根的依赖性及其接种效应研究[J].林业科学研究,2000,13(1)：8-14.[22]SARTORY D R,GROBBELAAR J U.Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis[J].Hydrobiologia,1984,114：177-187.[23]郑坚,陈秋夏,金川,等.不同TTC法测定枫香等阔叶树容器苗根系活力探讨[J].浙江农业科学,2008(1)：39-42.[24]刘欢,姚拓,刘婷,等.不同丛枝菌根真菌对苜蓿生长的影响[J].草原与草坪,2017,37(4)：61-67,73.[25]JOHNSON N C,GRAHAM J H,SMITH F A.Functioning of mycorrhzizal associations along the mutualism-parasitism continuum[J].New Phytologist,1997,135：575-585.[26]王怀玉,罗英.基质对VA菌根真菌的侵染及孢子产量的影响[J].四川师范大学学报(自然科学版),2003(3):302-305.[27]VIVAS A,MARULADA A,RUIZ-LOZANO J M,et al.Influence of a Bacillus sp.on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG-induced drought stress[J].Mycorrhiza,2003,13(5)：249-256.[28]XAVIER L J C,GERMIDA J J.Bacteria associated with Glomus clarum spores influence mycorrhizal activity[J].Soil Biology&Biochemistry,2003,35(3)：471-478.[29]GAO X P,GUO H H,ZHANG Q,et al.Arbuscular mycorrhizal fungi (AMF) enhanced the growth,yield,fiberquality and phosphorus regulation in upland cotton (Gossypium hirsutum L.)[J].Scientific Reports,2020,10(1)：2084.[30]HABIBZADEH Y,PIRZAD A,ZARDASHTI M R,et al.Effectsof arbuscular mycorrhizal fungi on seed and protein yieldunder water-deficit stress in mung bean[J].Agronomy Journal,2013,105(1):79-84.[31]晏梅静,补春兰,黄盖群,等.丛枝菌根真菌对桑树(Morus alba)地上部分的促进作用[J].植物生理学报,2020,56(12):2647-2654.[32]VEIGA R S T,van DER HEIJDEN M G A,FACCIO A.Arbuscular mycorrhizal fungi reduce growth and infect roots of the non-host plant Arabidopsis thaliana[J].Plant Cell and Environment,2013(36)：1926-1937.[33]闫萌萌,王铭伦,王洪波,等.光质对花生幼苗叶片光合色素含量及光合特性的影响[J].应用生态学报,2014,25(2)：483-487.[34]ZHANG H,XU N,LI X,et al.Arbuscular mycorrhizal fungi(Glomus mosseae) improves growth,photosynthesis and protects photosystem Ⅱ in leaves of Lolium perenne L.in cadmium contaminated soil[J].Frontiers in Plant Science,2018(9)：1156.[35]PARADISO R,ARENA C,MICCO V,et al.Changes in leaf anatomical traits enhanced photosynthetic activity of soybeangrown in hydroponics with plant growth-promoting microorganisms[J].Frontiers in Plant Science,2017(8)：674.[36]ZUBEK S,ROLA K,SZEWCZYK A,et al.Enhanced concentrations of elements and secondary metabolites in Viola tricolor L.induced by arbuscular mycorrhizal fungi[J].Plant and Soil,2015,390(1-2)：129-142.[37]吴强盛,袁芳英,费永俊,等.丛枝菌根真菌对白三叶根系构型和糖含量的影响[J].草业学报,2014,23(1)：199-204.[38]许朋阳,陈伟立,朱红惠,等.丛枝菌根真菌、磷和生长素对枳侧根形成的调控效应[J].亚热带植物科学,2016,45(3)：216-220.[39]薛英龙,李春越,王苁蓉,等.丛枝菌根真菌促进植物摄取土壤磷的作用机制[J].水土保持学报,2019,33(6)：12-22.
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备注/Memo
第一作者简介：王磊（1996-），男，硕士研究生，研究方向为植物生态学。E-mail：wangl788@nenu.edu.cn.责任作者：唐占辉（1980-），男，博士，副教授，现主要从事植物生态等研究工作。E-mail：tangzh789@nenu.edu.cn.基金项目：国家自然科学基金资助项目（31470446，31960228）。收稿日期：2021-03-24