ZHOU Min,CHENG Youpu,ZHANG Ying,et al.Effects of Four Cupric Pesticides on Antioxidant Enzyme Activities of Chinese Cabbage[J].Northern Horticulture,2021,(08):37-41.[doi:10.11937/bfyy.20202579]
四种铜制剂对小白菜抗氧化酶活性的影响
- Title:
- Effects of Four Cupric Pesticides on Antioxidant Enzyme Activities of Chinese Cabbage
- Keywords:
- Chinese cabbage; cupric pesticides; antioxidant enzymes
- 文献标志码:
- A
- 摘要:
- 以“早熟五号”小白菜为试材,采用盆栽方式,设定4种处理浓度,通过测定小白菜叶片超氧化物歧化酶(SOD),过氧化物酶(POD),过氧化氢酶(CAT)3种抗氧化酶活性的变化,研究了4种铜制剂对小白菜抗氧化酶活性的影响,以期为铜制剂在蔬菜生长中的安全使用提供参考依据。结果表明:1 800 mg?L-1王铜药后1 d、2 586 mg?L-1氧化亚铜药后2 d开始对小白菜产生药害,噻森铜和噻菌铜在研究浓度条件下对小白菜生长安全。推荐剂量下4种铜制剂对小白菜体内抗氧化酶活性均有一定影响,其中以SOD最为敏感,可将其作为小白菜受到铜制剂胁迫时的标记物,预防药害产生。4种药剂中王铜对小白菜最容易产生药害,氧化亚铜其次,而噻菌铜和噻森铜对小白菜相对安全。
- Abstract:
- Chinese cabbage variety of ‘Zaoshu No5’ was used as experimental material,with the potting experiment,four treatment concentrations were investigated.The effects of four cupric pesticides on the antioxidant enzyme activities of Chinese cabbage were studied by measuring the changes in activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT),to provide scientific guidance for the safe use of cupric pesticides in the growth of vegetables.The experiment results showed that 1 800 mg?L-1 copper oxychloride 1 days and 2 586 mg?L-1 cuprous oxide two days produce phytotoxicity,and thiodiazole-copper and saisen tong were safe for Chinese cabbage growth at the studied concentrations.The four cupric pesticides at the recommended concentration had certain effects on the activities of antioxidant enzymes in Chinese cabbage.The superoxide dismutase (SOD) was the most sensitive and could be used as a marker for Chinese cabbage under copper stress to prevent drug damage.In the four agents,copper oxychloride was the most susceptible to drug damage to Chinese cabbage,followed by cuprous oxide,while thiodiazole-copper and saisen tong were relatively safe to cabbage.
参考文献/References:
[1]华乃震.保护性杀菌剂中的四个杰出楷模述评[J].农药市场信息,2019(3):6-10,29.[2]李金堂,默书霞,王秋生,等.蔬菜铜制剂药害的发生及防治[J].长江蔬菜,2012(7):45,65.[3]惠隽雄.铜制剂在防治苹果病害中产生药害原因分析及对策[C].长沙:第二十一届全国农药械“双交会”,2005.[4]SARVAJEET S G,NARENDRA T.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12):909-930.[5]刘井兰,于建飞,印建莉,等.化学农药对植物生理生化影响的研究进展[J].农药,2006(8):511-514.[6]张清智,陈振德,刘红玉.氰戊菊酯对小白菜抗氧化酶活性及丙二醛(MDA)含量的影响[J].生态毒理学报,2008,3(5):507-512.[7]宋家永,李英涛,高飞,等.高效氯氟氰菊酯对小白菜保护酶活性及脂质过氧化作用的影响[J].江西农业大学学报,2013,35(5):920-923.[8]李海峰,王瑞华,韩琛.农药胁迫对植物抗氧化系统的研究现状[J].农产品加工,2018(3):59-62.[9]石连旋,颜宏.植物生理学试验指导[M].北京:高等教育出版社,2013.[10]MCCORD J M,FRIDOVICH I.Superoxide dismutase.An enzymic function for erythrocuprein (hemocuprein)[J].The Journal of Biological Chemistry,1969,244(22):6049-6055.[11]李英涛.上海青对4种不同类型农药胁迫的抗氧化防御反应研究[D].郑州:河南农业大学,2013.[12]ZHANG S,QIU C B,ZHOU Y,et al.Bioaccumulation and degradation of pesticide fluroxypyr are associated with toxic tolerance in green alga Chlamydomonas reinhardtii[J].Ecotoxicology,2011,20(2):337-347.[13]WILLEKENS H,CHAMNONGPOL S,DAVEY M,et al.Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants[J].The EMBO Journal,1997,16(16):4806-4816.[14]IRWIN F.Oxygen is toxic[J].Bio Science,1977,27(7):462.[15]张英鹏,林咸永,章永松,等.不同氮素形态对菠菜生长及体内抗氧化酶活性的影响[J].浙江大学学报(农业与生命科学版),2006(2):139-144.[16]曲爱军,郭丽红,孙绪艮,等.农药胁迫对大叶黄杨SOD和脯氨酸含量的影响[J].农药,2006(1):35-37.[17]徐敦明,马志卿,冯俊涛,等.毒死蜱和鬼臼毒素胁迫对蔬菜上海青抗氧化酶系及丙二醛的影响[J].农业环境科学学报,2004(6):1089-1092.[18]张清智,陈振德,王文娇,等.毒死蜱胁迫对小白菜抗氧化酶活性和相关生理指标的影响[J].生态学报,2008,28(9):4524-4530.
相似文献/References:
[1]陈祥伟,刘世琦,刘 庆,等.不同LED光源对小白菜生长及光合特性的影响[J].北方园艺,2013,37(22):1.
CHEN Xiang-wei,LIU Shi-qi,LIU Qing,et al.Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Pakchoi[J].Northern Horticulture,2013,37(08):1.
[2]王文全,周 颖,阿依丁别克.再生水灌溉及污泥施肥对蔬菜种子发芽及幼苗生长的影响[J].北方园艺,2013,37(22):181.
WANG Wen-quan,ZHOU Ying,Ayidingbieke.Influence of Reclaimed Water Irrigation and Sludge Fertilizer on Seed Germination and Seedling Growth of Vegetables[J].Northern Horticulture,2013,37(08):181.
[3]付雪清,李红利,朱翠敏,等.水杨酸和亚硫酸氢钠降低小白菜硝酸盐累积的复合效应研究[J].北方园艺,2014,38(04):9.
FU Xue-qing,LI Hong-li,ZHU Cui-min,et al.The Compound Effect of SA and Sodium Bisulfite on Reducing Nitrate Accumulation in the Leaf of Non-heading Chinese Cabbage[J].Northern Horticulture,2014,38(08):9.
[4]李雪芳,王林权,尚浩博,等.小白菜和小青菜对镉、汞、砷的富集效应及影响因素[J].北方园艺,2014,38(01):16.
LI Xue-fang,WANG Lin-quan,SHANG Hao-bo,et al.The Enrichment Effect of Cd,Hg and As onBrassica chinensisandBrassica pekinensisand Its Influencing Factors[J].Northern Horticulture,2014,38(08):16.
[5]李晓梅,赵红香,盛积贵.农药乙酰甲胺磷和高效氟氯氰菊酯对小白菜生理生化指标的影响[J].北方园艺,2013,37(06):132.
LI Xiao-mei,ZHAO Hong-xiang,SHENG Ji-gui.Effect of Organophosphorus Pesticide Acephate and Pyrethroid Pesticide Efficient Cyhalothrin on Physiological and Biochemical Index of Chinese Cabbage[J].Northern Horticulture,2013,37(08):132.
[6]张菊平,焦新菊,崔文朋,等.不同品种小白菜对镉胁迫的生理性差异[J].北方园艺,2013,37(08):9.
ZHANG Ju-ping,JIAO Xin-ju,CUI Wen-peng,et al.Physiological Differences of Different Chinese Cabbage Varieties on Cadmium Stress[J].Northern Horticulture,2013,37(08):9.
[7]刘 忠,蒋健明,莫士力,等.微量元素水溶性肥料对小白菜农艺性状及产量的影响[J].北方园艺,2013,37(20):155.
LIU Zhong,JIANG Jian-ming,MO Shi-li,et al.Effects of Microelement Water Soluble Fertilizer on Cabbage Agronomic Traits and Yield[J].Northern Horticulture,2013,37(08):155.
[8]肖丽,匡银近,覃彩芹.壳低聚糖浸种对镉胁迫下小白菜幼苗部分生理生化特性的影响[J].北方园艺,2012,36(17):27.
XIAO Li,KUANG Yin-jin,QIN Cai-qin.Influence of Oligochitosan on Some Physiological and Biochemical Characteristics of Chinese Cabbage under the Stress of Cadmium[J].Northern Horticulture,2012,36(08):27.
[9]刘朝阳,穆金艳,孙丽,等.小白菜主要营养品质及与农艺性状的相关分析[J].北方园艺,2014,38(15):10.
LIU Chao-yang,MU Jin-yan,SUN Li,et al.The Correlation Analysis on Main Nutrient Quality and Agronomic Traits of Pakchoi[J].Northern Horticulture,2014,38(08):10.
[10]孙丽,赵锋亮,李贞霞,等.二十二份小白菜主要农艺性状的遗传及相关分析[J].北方园艺,2014,38(17):1.
SUN Li,ZHAO Feng-liang,LI Zhenxia,et al.Genetic Analysis and Correlation Analysis on Agronomic Traits of Twenty-two Chinese Cabbage[J].Northern Horticulture,2014,38(08):1.
备注/Memo
第一作者简介:周敏(1996-),女,硕士研究生,研究方向为农药学。E-mail:1754772805@qq.com.责任作者:程有普(1969-),男,博士,副教授,现主要从事农药等研究工作。E-mail:chengyoupu@tjau.edu.cn.基金项目:天津市企业科技特派员资助项目(19JCTPJC59000);国家自然科学基金资助项目(31572034)。收稿日期:2020-06-20