[1]邓竹英,梁大成.拟南芥/本生烟远缘嫁接体中移动性mRNA分子检测[J].北方园艺,2020,44(16):22-28.[doi:10.11937/bfyy.20194602]
　DENG Zhuying,LIANG Dacheng.Identification of Mobile mRNAs in Arabidopsis/Nicotiana benthamiana Hetero-grafts[J].Northern Horticulture,2020,44(16):22-28.[doi:10.11937/bfyy.20194602]

点击复制

拟南芥/本生烟远缘嫁接体中移动性mRNA分子检测

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷期数: 2020年16 页码: 22-28 栏目: 出版日期: 2020-08-30

Title:: Identification of Mobile mRNAs in Arabidopsis/Nicotiana benthamiana Hetero-grafts

作者:: 邓竹英1; 2; 梁大成1; 2; （1.长江大学湖北省主要粮食作物产业化协同创新中心，湖北荆州 434025；2.长江大学湿地生态与农业利用教育部工程研究中心/湖北省涝渍灾害与湿地农业重点实验室，湖北荆州 434025）

Author(s):: DENG Zhuying1; 2; LIANG Dacheng1; 2; (1.Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou,Hubei 434025；2.Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Yangtze University/Hubei Key Laboratory of Waterlogging Disaster and Wetland Agriculture,Jingzhou,Hubei 434025)

关键词:: 拟南芥; 本生烟草; 远缘嫁接; 转录组测序; mRNA分子移动

Keywords:: Arabidopsis; Nicotiana benthamiana; hetero-grafting; RNA-Seq; mobile mRNA

DOI:: 10.11937/bfyy.20194602

文献标志码:: A

摘要:: 以拟南芥和本生烟草为试材，采用微嫁接技术和转录组测序的方法，研究了拟南芥/本生烟烟草远缘嫁接体中可移动的mRNA分子，以期进一步了解根茎间的信息交流机制。结果表明：在本生烟砧木中检测出824个向下移动的拟南芥mRNA分子，挑选其中30个高丰度的拟南芥mRNA靶分子进行RT-PCR扩增验证，未发现可以移动的mRNA分子。进一步验证其中psbW和[STBX]RBCS1A[STBZ]基因均未发生移动。利用克隆测序，检测出[STBX]AT1G26630(elF5A2[STBZ])的序列来自于本生烟草，而非接穗部分移动而来。深度测序得到的可移动mRNAs并未能够被RT-PCR技术所验证，上述结果表明mRNA可能并非担当根茎间长距离运输的角色。

Abstract:: Arabidopsis and Nicotiana benthamiana were used as materials,by deploying the micrografting technique and deep-sequencing platform,the possibility of potentially mobile mRNAs between Arabidopsis scion and Nicotiana rootstock were explored in order to understand the mechanisms of root/shoot communication.The results showed that 824 transcripts belonging to Arabidopsis were identified in the Nb rootstock about deep sequencing,suggesting that mRNA might move across the graft union.To further confirm the mobility of the identified Arabidopsis transcripts,RT-PCR analyses of 30 high-potential mobile transcripts together with previously identified psbW and RBCS1A transcripts were performed.The amplified PCR fragment for AT1G26630 (elF5A2) gene was further cloned and sequenced,and it was from Nicotiana benthamiana rather than from the scion.Mobile mRNA candidates identified with deep sequencing technique cannot be validated by RT-PCR,thereby presenting concerns about the role of mRNAs in acting as long-distance signaling between shoot and root as previously suggested.

参考文献/References:

[1]THIEME C J,ROJAS-TRIANA M,STECYK E,et al.Endogenous Arabidopsis messenger RNAs transported to distant tissues[J].Nature Plants,2015,1(4):15025.[2]SPIEGELMAN Z,GOLAN G,WOLF S.Don′t kill the messenger:Long-distance trafficking of mRNA molecules[J].Plant Science,2013,213:1-8.[3]BANERJEE A K,CHATTERJEE M,YU Y Y,et al.Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway[J].The Plant Cell,2006,18(12):3443-3457.[4]LOUGH T J,LUCAS W J.Integrative plant biology:Role of phloem long-distance macromolecular trafficking[J].Annual Review of Plant Biology,2006,57(1):203-232.[5]TURGEON R,WOLF S.Phloem transport:Cellular pathways and molecular trafficking[J].Annual Review of Plant Biology,2009,60(1):207-221.[6]ATKINS C A,SMITH P M C,RODRIGUEZ-MEDINA C.Macromolecules in phloem exudates:A review[J].Protoplasma,2011,248(1):165-172.[7]LIN M K,BELANGER H,LEE Y J,et al.Floweing locus T protein May act as the long-distance florigenic signal in the cucurbits[J].The Plant Cell,2007,19(5):1488-1506.[8]CORBESIER L,VINCENT C,JANG S,et al.FT protein movement contributes to long-distance signaling in floral induction of arabidopsis[J].Science,2007,316(5827):1030-1033.[9]HUEN A K,RODRIGUEZ-MEDINA C,HO A Y Y,et al.Long-distance movement of phosphate starvation-responsive microRNAs in Arabidopsis[J].Plant Biology,2017,19(4):643-649.[10]KHALDUN A B M,HUANG W J,LV H,et al.Comparative profiling of miRNAs and target gene identification in distant-grafting between tomato and Lycium(goji berry)[J].Frontiers in Plant Science,2016(7):1475.[11]PAGLIARANI C,VITALI M,FERRERO M,et al.The accumulation of miRNAs differentially modulated by drought stress is affected by grafting in grapevine[J].Plant Physiology,2017,173(4):2180-2195.[12]BROSNAN C A,VOINNET O.Cell-to-cell and long-distance siRNA movement in plants:Mechanisms and biological implications[J].Current Opinion in Plant Biology,2011,14(5):580-587.[13]LIANG D C,WHITE R G,WATERHOUSE P M.Gene silencing in Arabidopsis spreads from the root to the shoot,through a gating barrier,by template-dependent,nonvascular,cell-to-cell movement[J].Plant Physiology,2012,159(3):984-1000.[14]ZHANG W N,KOLLWIG G,STECYK E,et al.Graft-transmissible movement of inverted-repeat-induced siRNA signals into flowers[J].The Plant Journal,2014,80(1):106-121.[15]KIM G,LEBLANC M L,WAFULA E K,et al.Genomic-scale exchange of mRNA between a parasitic plant and its hosts[J].Science,2014,345(6198):808-811.[16]HUANG N C,YU T S.The sequences of Arabidopsis GA-insensitive RNA constitute the motifs that are necessary and sufficient for RNA long-distance trafficking[J].The Plant Journal,2009,59(6):921-929.[17]HAYWOOD V,YU T S,HUANG N C,et al.Phloem long-distance trafficking of gibberellic acid insensitive RNA regulates leaf development[J].The Plant Journal,2005,42(1):49-68.[18]YANG H W,YU T S.Arabidopsis floral regulators FVE and AGL24 are phloem-mobile RNAs[J].Botanical Studies,2010,51(1):17-26.[19]YANG Y Z,MAO L Y,JITTAYASOTHORN Y,et al.Messenger RNA exchange between scions and rootstocks in grafted grapevines[J].BMC Plant Biology,2015,15:251.[20]XU H Y,ZHANG W N,LI M F,et al.Gibberellic acid insensitive mRNA transport in both directions between stock and scion in malus[J].Tree Genetics & Genomes,2010,6(6):1013-1019.[21]XU H Y,IWASHIRO R,LI T Z,et al.Long-distance transport of gibberellic acid insensitive mRNA in Nicotiana benthamiana[J].BMC Plant Biology,2013,13(1):165.[22]LEBLANC M,KIM G,PATEL B,et al.Quantification of tomato and Arabidopsis mobile RNAs trafficking into the parasitic plant Cuscuta pentagona[J].New Phytologist,2013,200(4):1225-1233.[23]HUANG N C,LUO K R,YU T S.Mobility of antiflorigen and PEBP mRNAs in Toma to-tobacco heterografts[J].Plant Physiology,2018,178(2):783-794.[24]XIA C,ZHENG Y,HUANG J,et al.Elucidation of the mechanisms of long-distance mRNA movement in a Nicotiana benthamiana/tomato heterograft system[J].Plant Physiology,2018,177(2):745-758.[25]NOTAGUCHI M,OKAMOTO S.Dynamics of long-distance signaling via plant vascular tissues[J].Frontiers in Plant Science,2015（6）:161.[26]ZHANG Z L,ZHENG Y,HAM B K,et al.Vascular-mediated signalling involved in early phosphate stress response in plants[J].Nature Plants,2016,2(4):16033.[27]JAILANI A A K,SOLANKI V,ROY A,et al.A CGMMV genome-replicon vector with partial sequences of coat protein gene efficiently expresses GFP in Nicotiana benthamiana[J].Virus Research,2017,233:77-85.

相似文献/References:

[1]陈昱,官丽莉,杨晶,等.植物金属硫蛋白的研究进展[J].北方园艺,2014,38(07):185.
　CHEN Yu,GUAN Li-li,YANG Jing,et al.Research Progress on Plant Metallothionein[J].Northern Horticulture,2014,38(16):185.
[2]郭涛１,２,晁跃辉,等.拟南芥NCED4基因的克隆及初步功能鉴定[J].北方园艺,2014,38(17):105.
　GUO Tao,CHAO Yue-hui,et al.Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana[J].Northern Horticulture,2014,38(16):105.
[3]王崇,程玉祥.AtGDPD-Like 6和AtGDPD-Like 7基因启动子表达特性及AtGDPD-Like 6蛋白定位[J].北方园艺,2015,39(11):90.[doi:10.11937/bfyy.201511022]
　WANG Chong,CHENG Yu-xiang.The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6[J].Northern Horticulture,2015,39(16):90.[doi:10.11937/bfyy.201511022]
[4]杨淑萍,马伟琴,刘阳,等.模式生物拟南芥研究进展的文献计量分析[J].北方园艺,2015,39(19):195.[doi:10.11937/bfyy.201519049]
　YANG Shuping,MA Weiqin,LIU Yang,et al.Bibliometric Analysis of Research Progress on Model Species Arabidopsis thaliana[J].Northern Horticulture,2015,39(16):195.[doi:10.11937/bfyy.201519049]
[5]周　索,杜　丽.四种生长素对拟南芥根生长发育的影响[J].北方园艺,2008,32(03):0.[doi:10.11937/bfyy.200803024]
　ZHOU Suo,DU Li.Study on the Root Growth and Development of Arabidopsis thaliana[J].Northern Horticulture,2008,32(16):0.[doi:10.11937/bfyy.200803024]
[6]张亮,马灵玉,王太霞.低剂量60Co-γ辐照刺激拟南芥植株生长的效应[J].北方园艺,2016,40(23):28.[doi:10.11937/bfyy.201623007]
　ZHANG Liang,MA Lingyu,WANG Taixia.Effect of Low-dose Gamma Irradiation on Growth and Development of Arabidopsis Plants[J].Northern Horticulture,2016,40(16):28.[doi:10.11937/bfyy.201623007]
[7]宿强,曾会明,许立新,等.紫花苜蓿MsSAG[STBX]113基因的克隆及对拟南芥的转化[J].北方园艺,2018,42(06):10.[doi:10.11937/bfyy.20172691]
　XU Qiang,ZENG Huiming,XU Lixin,et al.Cloning of MsSAG113 Gene From Medicago sativa L. and Transformation of Arabidopsis thaliana[J].Northern Horticulture,2018,42(16):10.[doi:10.11937/bfyy.20172691]
[8]唐尧,张微,尹艳莉,等.拟南芥[STBX]AtOFP8[STBZ]的生物信息学分析及表达分析[J].北方园艺,2018,42(18):35.[doi:10.11937/bfyy.20174346]
　TANG Yao,ZHANG Wei,YIN Yanli,et al.Bioinformatics Analysis and Expression Analysis of AtOFP8 in Arabidopsis thaliana[J].Northern Horticulture,2018,42(16):35.[doi:10.11937/bfyy.20174346]
[9]高润昕,黄凤珍,李萌,等.水势和盐胁迫对拟南芥种子萌发和根部生长的影响[J].北方园艺,2019,43(15):8.[doi:10.11937/bfyy.20184111]
　GAO Runxin,HUANG Fengzhen,LI Meng,et al.Effects of Water Potential and Salt Stress on Seed Germination and Root Growth in Arabidopsis[J].Northern Horticulture,2019,43(16):8.[doi:10.11937/bfyy.20184111]

备注/Memo

第一作者简介：邓竹英（1991-），女，博士研究生，研究方向为生物大分子长距离信号运输现象。E-mail：201572341@yangtzeu.edu.cn.责任作者：梁大成（1979-），男，博士，教授，现主要从事嫁接机理及生物大分子长距离信号运输现象等研究工作。E-mail：dachengliang@gmail.com.基金项目：国家自然科学基金资助项目（31671257）。收稿日期：2019-12-05

更新日期/Last Update: 2020-11-23