[1]李晓科,武玉珍,张谨华,等.外源H2S对镉胁迫下菠菜种子萌发及幼苗生长的影响[J].北方园艺,2020,44(16):16-21.[doi:10.11937/bfyy.20194934]
　LI Xiaoke,WU Yuzhen,ZHANG Jinhua,et al.Effects of Hydrogen Sulfide on Seed Germination and Seedling Growth of Spinach Under Cadmium Stress[J].Northern Horticulture,2020,44(16):16-21.[doi:10.11937/bfyy.20194934]
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外源H2S对镉胁迫下菠菜种子萌发及幼苗生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年16 页码: 16-21 栏目: 出版日期: 2020-08-30
Title:
Effects of Hydrogen Sulfide on Seed Germination and Seedling Growth of Spinach Under Cadmium Stress
作者:
李晓科1; 武玉珍1; 张谨华1; 张义贤2
（1.晋中学院 生物科学与技术学院，山西 晋中 030619；2.山西大学 生命科学学院，山西 太原 030006）
Author(s):
LI Xiaoke1; WU Yuzhen1; ZHANG Jinhua1; ZHANG Yixian2
(1.School of Biology Science and Technology，Jinzhong College，Jinzhong，Shanxi 030619；2.School of Life Science，Shanxi University，Taiyuan，Shanxi 030006)
关键词:
Cd胁迫; H2S; 菠菜; 种子萌发; 幼苗生长
Keywords:
cadmium stress; H2S; spinach; seed germination; seedling growth
DOI:
10.11937/bfyy.20194934
文献标志码:
A
摘要:
为了探明外源H2S对镉胁迫下菠菜的适应性反应特性，以菠菜为试材，采用室内培养法，研究了外源NaHS（H2S的供体）对镉胁迫下菠菜种子萌发指标的影响及幼苗的生长响应，以期为镉污染地区菠菜的栽培管理提供参考依据。结果表明：在重金属镉（0.36 mmol?L-1）和NaHS溶液（40 μmol?L-1）以及H2S生成抑制剂HA溶液（0.8 mmol?L-1）处理下，与对照相比，镉和HA单独处理使菠菜种子的萌发指标（发芽率、发芽势、发芽指数、活力指数、异状发芽率）、幼苗生长指标（根长、株高、生物量、根系耐性指数）和呼吸速率显著降低。随着H2S的加入，对以上各项指标有促进作用，其中复合处理组（NaHS＋Cd）表现出H2S对镉伤害的缓解作用。而萌发生理指标丙二醛（MDA）含量在镉和HA单独处理显著升高，H2S的加入使其含量下降，缓解了自由基对菠菜种子的伤害。
Abstract:
In order to ascertain the exogenous H2S adaptive response characteristics of spinach under Cd stress,the effects of exogenous NaHS (H2S donor) on seed germination index and seedling growth response of spinach under Cd stress were studied by using spinach as experimental material and adopting indoor culture method,so as to provide experimental theoretical basis for planting and management of spinach in the Cd polluted areas.The results showed that in the treatment of heavy metal Cd (0.36 mmol?L-1),NaHS solution (40 μmol?L-1) and H2S generation inhibitor HA solution (0.8 mmol?L-1),compared with the control,spinach seed germination indexes (germination rate,germination potential,germination index,vigor index,unusual germination rate),seedling growth indexes (root length,plant height,biomass,root system tolerance index) and respiratory rate were reduced significantly under the treatment of Cd and HA alone.With the addition of H2S,it had a promoting effect on the above indexes,especially the compound treatment group (NaHS+Cd) showed the mitigating effect of H2S on Cd damage.The content of malondialdehyde (MDA),a physiological index of germination,was significantly increased under the treatment of Cd and HA alone,while the addition of H2S reduced MDA content and alleviated the damage of ROS to spinach seeds.

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备注/Memo

第一作者简介：李晓科（1979-），男，硕士，讲师，现主要从事植物生理生态等研究工作。E-mail：jzxylixiaoke@163.com.基金项目：山西省自然科学基金资助项目（201701D121083）；晋中学院博士基金资助项目（2015006）。收稿日期：2019-12-30

更新日期/Last Update: 2020-11-23