[1]郭涛１,２,晁跃辉,等.拟南芥NCED4基因的克隆及初步功能鉴定[J].北方园艺,2014,38(17):105-110.
　GUO Tao,CHAO Yue-hui,et al.Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana[J].Northern Horticulture,2014,38(17):105-110.

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拟南芥NCED4基因的克隆及初步功能鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年17期页码: 105-110 栏目: 出版日期: 2014-09-15

Title:: Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana

文章编号:: 1001-0009(2014)17-0105-06

作者:: 郭涛^１; ２; 晁跃辉²; 杨青川2; 金洪¹; 张铁军²; 康俊梅²; １.内蒙古农业大学生态环境学院，内蒙古呼和浩特 010019；
２.中国农业科学院北京畜牧兽医研究所，北京 100193

Author(s):: GUO Tao^１; ２; CHAO Yue-hui²; YANG Qing-chuan²; JIN Hong¹; ZHANG Tie-jun²; KANG Jun-mei²; 1.Collefe of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010019；
2. Beijing Institute of Animal Husbandry and Veterinary,Chinese Academy of Agricultural Sciences,Beijing 100193

关键词:: 拟南芥; NCED4; 脱落酸; 转化

Keywords:: Arabidopsis thaliana; NCED4; abscisic acid; tranformation

分类号:: Q943.2

文献标志码:: A

摘要:: 以拟南芥为试材，采用PCR及DNA重组技术，从野生型拟南芥中克隆出NCED4基因，并构建植物表达载体pBI-NCED4，最终获得具有卡那霉素抗性的转基因拟南芥植株，研究NCED4基因超表达对植物体的影响，并对其中4个再生植株进行PCR和荧光定量RT-PCR分析。结果表明：目的基因转入拟南芥基因组中，并在转基因拟南芥中成功过量表达；NCED4基因受外源激素的影响较为敏感，其作用可能与激素的调控有关。

Abstract:: Taking Arabidopsis thaliana as material,a gene which was named as NCED4was cloned by PCR technique.NCED4 gene was inserted into plasmid and resulting a plant expressing vector pBI-NCED4 with DNA recombination technology.Transgenic Arabidopsis plants with kanamycin resistance were obtained finally,and this were the basis of researching that the effect of NCED4 gene overexpression on plant.The results showed that，PCR and Real Time RT-PCR analysis of 4 kanamycin resistant plants showed that the target gene was inserted into the genomes of the Arabidopsis thaliana and NCED4 gene was over-expressed in transgenic Arabidopsis successfully;the expression levels of NCED4 genes were changed obviously when exogenous hormones were added,and these results showed that NCED4 gene may play an important role in hormone biosynthesis and regulation.

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备注/Memo

第一作者简介：郭涛（1988-)，男，硕士研究生，研究方向为植物资源利用与保护。E-mail：yushen0002008@126.com.

责任作者：金洪（1958-)，男，博士，教授，研究方向为草业科学。E-mail:jinhong915@163.com.

基金来源：国家“十二五”科技支撑子课题资助项目（2011BAD17B01-01-3）；国家牧草产业体系资助项目（CARS-35-04，201）；中国农业科学院北京畜牧兽医研究所基本科研业务费资助项目（2014ywf-zd-2）。

更新日期/Last Update: 2014-09-26