[1]周伟坚,陈赛花,万俊华,等.荆西口蘑培养条件及栽培种培养基的优化[J].北方园艺,2020,44(15):124-129.[doi:10.11937/bfyy.20194158]
　ZHOU Weijian,CHEN Saihua,WAN Junhua,et al.Optimization of Culture Conditions and Culture Medium of Macrocybe gigantea[J].Northern Horticulture,2020,44(15):124-129.[doi:10.11937/bfyy.20194158]
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荆西口蘑培养条件及栽培种培养基的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年15 页码: 124-129 栏目: 出版日期: 2020-08-15
Title:
Optimization of Culture Conditions and Culture Medium of Macrocybe gigantea
作者:
周伟坚1; 陈赛花2; 万俊华3; 查丽燕1; 王越1; 莫美华1
（1.华南农业大学 食品学院，广东 广州 510642；2.广州菇王科技有限公司，广东 广州 510308；3.广州粤旺农业科技有限公司，广东 广州 510450）
Author(s):
ZHOU Weijian1; CHEN Saihua2; WAN Junhua3; ZHA Liyan1; WANG Yue1; MO Meihua1
(1.College of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642;2.Guangzhou Guwang Technology Limited Company,Guangzhou,Guangdong 510308;3.Guangzhou Yuewang Agricultural Science and Technology Limited Company,Guangzhou,Guangdong 510450)
关键词:
巨大口蘑; 培养基; 均匀设计; 优化
Keywords:
Macrocybe gigantea; medium; uniform design; optimization
DOI:
10.11937/bfyy.20194158
文献标志码:
A
摘要:
以蔗渣、棉籽壳、稻草、玉米秆、玉米芯、麸皮、酵母粉、石灰、KH2PO4、轻质碳酸钙为试材，采用均匀设计法研究荆西口蘑栽培种培养基配方，利用均匀设计表U12（1210）进行试验方案的设计，以菌丝体在袋子中的生长深度作为目标函数（Y）经DPS v9.50数据处理软件对数据进行二次回归分析，并对培养温度和pH进行了筛选，以期获得荆西口蘑栽培种培养基优化配方、菌丝适宜生长温度和pH。结果表明：荆西口蘑栽培种培养基的优化配方为蔗渣38.47%，棉籽壳29.24%，玉米秆6.59%，玉米芯15.25%，麸皮0.64%，酵母粉2.13%，石灰5.16%，磷酸二氢钾2.51%。最适生长温度为30 ℃，适宜酸碱度范围pH 6～9。
Abstract:
Bagasse,cottonseed shell,straw,corn stalk,corncob,bran,yeast powder,lime,KH2PO4 and light calcium carbonate were used as test materials.In order to obtain the optimum culture medium and the suitable growth temperature and pH of mycelium.Uniform design method was used to study the culture medium formula of Macrocybe giganteum,which was from Jingxi in Fujian Province.Uniform design table U12 (1210) was adopted to arrange the experiment scheme.The growth depth of M.gigantea mycelium in the bag was taken as the objective function (Y) and the data was analyzed by DPS v9.50 data processing software.The culture temperature and pH were screened.The results showed that the optimum culture medium was bagasse 38.47%,cottonseed shell 29.24%,corn stalk 6.59%,corncob 15.25%,bran 0.64%,yeast 2.13%,lime 5.16%,KH2PO4 2.51%.The optimum growth temperature is 30 ℃,and the suitable pH range is 6-9.

参考文献/References:

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备注/Memo

第一作者简介：周伟坚(1964-),男，本科，实验师，现主要从事食用真菌学和分子生物学等研究工作。E-mail:zwj85281192@scau.edu.cn.责任作者：莫美华(1966-)，女，博士，教授，现主要从事食用真菌学等研究工作。E-mail:mindymo@163.com.基金项目：广东省现代农业产业技术体系创新团队建设资助项目（2019KJ103）；广州市产学研合作创新重大资助项目(201704020020)；广东省科技创新战略专项资金（重点领域研发计划项目）资助项目（2018B020206001）；2016广东省农业标准化研究资助项目。收稿日期：2019-11-06

更新日期/Last Update: 2020-10-29