MA Mengli,ZHANG Wei,MENG Hengling,et al.Genetic Diversity Analysis of Amomum tsao-ko in Main Producing Area Using RAPD Markers[J].Northern Horticulture,2020,44(10):121-127.[doi:10.11937/bfyy.20193422]
利用RAPD标记分析主产地草果遗传多样性
- Title:
- Genetic Diversity Analysis of Amomum tsao-ko in Main Producing Area Using RAPD Markers
- 文献标志码:
- A
- 摘要:
- 以云南省红河州4个居群的48份草果为试材，采用RAPD分子标记技术，研究草果遗传多样性，以期为草果资源的保护及利用提供参考依据。结果表明：11条RAPD引物共扩增出139个条带，多态性条带比率(PPB)为98.56%。在群体水平上，金平居群的遗传多样性最高（PPB=82.01%），其次是绿春居群（PPB=66.91%），屏边居群（57.55%）和元阳居群（55.40%）多样性水平相对较低。草果总遗传多样性的87.67%(Hs=0.197)来自于居群内部，居群间遗传变异只占12.33%(Gst=0.123)，AMOVA分析进一步证明草果的遗传变异主要存在于居群内部。遗传一致度分析表明不同居群间的遗传一致度较高，相似性系数在0.931 4~0.971 7，表明草果居群间遗传变异较小。
- Abstract:
- The genetic diversity of 48 Amomum tsao-ko from four populations in Honghe Prefecture of Yunnan Province was analyzed by random amplified polymorphic DNA (RAPD) molecular marker technique.This study could provide an important theoretical basis for the protection and utilization of A.tsao-ko resources.The results showed that a total of 139 bands were amplified by 11 RAPD primers,and the ratio of polymorphic bands (PPB) was 98.56%.At the population level,Jinping population had the highest genetic diversity (PPB=82.01%),followed by Lvchun population (PPB=66.91%),Pingbian population (57.55%) and Yuanyang population (55.40%).87.67% (Hs=0.197) of the total genetic diversity of A.tsao-ko came from within populations,and the genetic variation among populations accounted for only 12.33%(Gst=0.123),analysis of molecular variance (AMOVA) further proved that the genetic variation of A.tsao-ko mainly existed within populations.Four A.tsao-ko populations shared high levels of genetic identity (0.931 4-0.971 7),and the genetic variation among the populations was small.
参考文献/References:
[1]LIM T K.Amomum tsao-ko,In edible medicinal and non-medicinal plants[M].Berlin:Springer Netherlands Press,2013.[2]LIU G,JIN M,CAI C,et al.Soil microbial community structure and physicochemical properties in Amomum tsao-ko based agroforestry systems in the Gaoligong Mountains,Southwest China[J].Sustainability,2019,11(2):546.[3]SHI Y N,JIN H,YANG Y,et al.Herbal verification of medicinal Amomum tsao-ko[J].Med Plant,2014,5(9):16-19,24.[4]杨绍兵,张金渝.草果生产加工适宜技术[M].北京:中国医药科技出版社,2018.[5]张拓.人工合成小麦遗传多样性及重要农艺性状变异分析[D].杨凌:西北农林科技大学,2017.[6]WILLIAMS J G K,KUBELIK A R,LIVAK K J,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Research,1990,18(22):6531-6535.[7]SHARMA S,KUMAR P,GAMBHIR G,et al.Assessment of genetic diversity in lettuce (Lactuca sativa L.) germplasm using RAPD markers[J].3 Biotech,2018,8(1):9.[8]KAUR P,PANDEY D K,GUPTA R C,et al.Assessment of genetic diversity among different population of five Swertia species by using molecular and phytochemical markers[J].Industrial Crops and Products,2019,138:111569.[9]PIDIGAM S,MUNNAM S B,NIMMARAJULA S,et al.Assessment of genetic diversity in yardlong bean (Vigna unguiculata (L.) Walp subsp.sesquipedalis Verdc.) germplasm from India using RAPD markers[J].Genetic Resources and Crop Evolution,2019,66(6),1231-1242.[10]LIU H,YAN Q,ZOU D,et al.Identification and bioactivity evaluation of ingredients from the fruits of Amomum tsaoko Crevost et Lemaire[J].Phytochemistry Letters,2018,28:111-115.[11]SIM S,TAN S K,KOHLENBERG B,et al.Amomum tsao-ko:Chinese Black Cardamom:Detailed oil composition and comparison with two other Cardamom species[J].Natural Product Communications,2019,14(7):1934578X19857675.[12]KIM J G,JANG H,LE T P L,et al.Pyranoflavanones and pyranochalcones from the fruits of Amomum tsao-ko[J].Journal of Natural Products,2019,82(7):1886-1892.[13]YANG Y W,YANG Z Y,YAN M R,et al.Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae),an economically important plant in China[J].Genetics and Molecular Research,2014,13(4):8220-8224.[14]谢正万,宁德鲁,周军,等.适于草果遗传多样性分析的RAPD引物筛选[J].西部林业科学,2018,47(4):45-50.[15]胡一凡,张雪梅,徐绍忠,等.云南草果种质资源的遗传多样性及亲缘关系的SSR分析[J].中草药,2018,49(22):163-170.[16]PEAKALL R O D,SMOUSE P E.GENALEX 6:Genetic analysis in excel.Population genetic software for teaching and research[J].Molecular Ecology Notes,2006,6(1):288-295.[17]ROBARTS D W H,WOLFE A D.Sequence-related amplified polymorphism (SRAP) markers:A potential resource for studies in plant molecular biology[J].Applications in Plant Sciences,2014,2(7):1400017.[18]徐吉银,丁平.道地药材阳春砂不同居群的RAPD分析[J].中药新药与临床药理,2005,16(3):194-196.[19]张忠廉,李学兰,杨春勇,等.砂仁遗传多样性的ISSR分析[J].中草药,2011,42(3):570-574.[20]BOOY G,HENDRIKS R J J,SMULDERS M J M,et al.Genetic diversity and the survival of populations[J].Plant Biology,2000,2(4):379-395.[21]NYBOM H.Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J].Molecular Ecology,2004,13(5):1143-1155.[22]LI M,CHEN X,HUANG M,et al.Genetic diversity and relationships of ancient Chinese fir (Cunninghamia lanceolata) genotypes revealed by sequence-related amplified polymorphism markers[J].Genetic Resources and Crop Evolution,2017,64(5):1087-1099.[23]陈宗游,黄夕洋,唐辉,等.广西甜茶种质资源遗传多样性的ISSR分析[J].园艺学报,2017,44(1):161-169.[24]姜武,吴志刚,陶正明,等.基于ISSR和SRAP标记的栀子种质遗传多样性研究[J].中草药,2019,50(2):251-257.[25]HAMRICK J L,GODT M J W.Effects of life history traits on genetic diversity in plant species[J].Philosophical Transactions of the Royal Society of London.Series B:Biological Sciences,1996,351(1345):1291-1298.[26]BUSSELL J.The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)[J].Molecular Ecology,1999(8):775-789.[27]崔晓龙,魏蓉城,黄瑞复.草果遗传体系的初步研究[J].云南大学学报(自然科学版),1995,17(3):290-297.[28]李国栋,张洪武,刘小莉,等.草果两型植株的繁育差异研究[J].广西植物,2017,37(10):1312-1321.
相似文献/References:
[1]肖志娟,翟梅枝,许静,等.不同核桃品种的ISSR标记分析[J].北方园艺,2013,37(04):103.
[2]魏姗姗,刘兴菊,杨敏生,等.基于成熟期的桃品种遗传多样性SSR分析[J].北方园艺,2014,38(12):88.
WEI Shan-shan,LIU Xing-ju,YANG Min-sheng,et al.Genetic Diversity of SSR Analysis of Prunus persica Cultivars Based on Maturity[J].Northern Horticulture,2014,38(10):88.
[3]刘志曦,李志强,魏海莲,等.平菇ISSR遗传多样性分析[J].北方园艺,2014,38(12):94.
LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(10):94.
[4]李 娜,张存旭,崔晓燕,等.栓皮栎ISSR反应体系的建立[J].北方园艺,2014,38(13):93.
LI Na,ZHANG Cun-xu,CUI Xiao-yan,et al.Establishment of ISSR Reaction System in Quercus variabilis [J].Northern Horticulture,2014,38(10):93.
[5]张文龙.石斛种质资源遗传多样性的PAGE分析[J].北方园艺,2014,38(14):104.
ZHANG Wen-long.PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources[J].Northern Horticulture,2014,38(10):104.
[6]桂明英,赵春艳,吴素蕊,等.黑木耳18S rDNA序列变异及遗传多样性分析[J].北方园艺,2014,38(14):111.
GUI Ming-ying,ZHAO Chun-yan,WU Su-rui,et al.Analyses of Sequence Variation and Genetic Diversity on 18S rDNA Regions of Auricularia auricula-judae[J].Northern Horticulture,2014,38(10):111.
[7]傅 冰,洪 震,刘跃钧,等.蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析[J].北方园艺,2013,37(17):95.
FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(10):95.
[8]龙 茜,刘洪章,刘树英.玉簪种质资源遗传多样性SSR分析[J].北方园艺,2013,37(17):100.
LONG Xi,LIU Hong-zhang,LIU Shu-ying.The Analysis of Hosta Resources Genetic Diversity by SSR[J].Northern Horticulture,2013,37(10):100.
[9]张建英,毛向红,张莹莹.核桃种质资源遗传多样性的RAMP标记分析[J].北方园艺,2014,38(09):106.
ZHANG Jian-ying,MAO Xiang-hong,ZHANG Ying-ying.Study on the Genetic Diversity of Walnut Germplasm Resources Based on RAMP Marker[J].Northern Horticulture,2014,38(10):106.
[10]甘 娜,陈其兵,罗承德.四川天彭牡丹遗传多样性研究[J].北方园艺,2014,38(09):112.
GAN Na,CHEN Qi-bing,LUO Cheng-de.Research on the Genetic Diversity of Tianpeng Tree Peony[J].Northern Horticulture,2014,38(10):112.
备注/Memo
第一作者简介：马孟莉（1985-），女，硕士，讲师，研究方向为植物遗传学。E-mail：mamlsky@126.com.责任作者：卢丙越（1981-），男，博士，副教授，研究方向为植物分子遗传学。E-mail：lby202@126.com.基金项目：国家自然科学基金资助项目（31460380）；红河学院中青年学术骨干培养资助项目（2014GG0101）；红河学院重点学科开放基金资助项目（2018ZDXK02）；红河学院大学生创新创业训练计划资助项目（DCXL181073）。收稿日期：2019-12-01