YUE Wansong,GUO Xiang,FENG Yunli,et al.Isolation and Identification of Two Wild Strains of Lyophyllum spp.and Selection of Liquid Culture Medium[J].Northern Horticulture,2020,44(02):124-129.[doi:10.11937/bfyy.20192616]
两个离褶伞野生菌株的鉴定及其液体培养基的初步筛选
- Title:
- Isolation and Identification of Two Wild Strains of Lyophyllum spp.and Selection of Liquid Culture Medium
- 文献标志码:
- A
- 摘要:
- 以2个分离自云南鸡足山的野生离褶伞菌株C821-001和C822-003为试材，根据ITS序列分析，对2个野生菌株进行分类鉴定。采用液体摇瓶培养法，以菌丝生物量为主要指标，对C821-001和C822-003进行液体培养基筛选，从5种不同培养基中分别筛选出适合2个菌株菌丝生长的液体培养基。结果表明：经ITS序列分析证实C821-001为真姬离褶伞Lyophyllum shimeji，C822-003为烟熏褐离褶伞Lyophyllum infumatum。C821-001和C822-003均在配方5中生长最好，菌丝体干质量分别达到1.948 8 g和1.128 8 g，因此，配方5可作为2个菌株菌丝体繁殖的首选液体培养基。
- Abstract:
- Two wild strains,named C821-001 and C822-003,were isolated from two spieces of Lyophyllum spp.which were collected in Jizushan of Yunnan Province，then these two selected samples were identified by ITS sequence analysis.The liquid culture medium of C821-001 and C822-003 were selected by liquid shaking flask culture method with mycelial biomass as the main index,the liquid culture medium suitable for mycelial growth of two selected strains were selected from 5 kinds of culture medium which consisted of different materials.The results showed that C821-001 and C822-003 were respectively identified as Lyophyllum shimeji and Lyophyllum infumatum analyzed by phylogenetic relationship of ITS sequences.Both C821-001 and C822-003 grew best in formula 5,with the dry weight of mycelium 1.948 8 g and 1.128 8 g，respectively.So the formula 5 could be used as the preferred liquid culture medium for mycelium propagation of two selected strains.
参考文献/References:
[1]KIRK P M，CANNON P F，MINTER D W,et al.Dictionary of the fungi (10th ed)[M].Wallingford，UK:CABI Publishing，2008.[2]卯晓岚.中国大型真菌[M].郑州:河南科学技术出版社，2000.[3]桂明英,马绍斌,郭相,等.西南大型真菌(Ⅰ)[M].上海:上海科学技术文献出版社,2016.[4]李永红.云南离褶伞菌株的ITS鉴定及其生理研究[D].昆明:西南林学院，2009.[5]黄兴奇.云南作物种质资源[M].昆明:云南科技出版社,2005.[6]孙权,沈玉秀,沈明花.榆干离褶伞发酵液体外抗氧化性能研究[J].食品科技，2010，35(1):223-225.[7]李晓,张士颖,李玉.灰离褶伞子实体营养成分测定与评价[J].食用菌,2010(6):198-201.[8]金梅花,沈玉秀,沈明花.榆干离褶伞发酵液对急性肝损伤的保护作用[J].食品科技，2010，35(4):78-80.[9]魏雨恬,冯娜,张劲松,等.荷叶离褶伞子实体有机溶剂萃取物的化学成分和抗肿瘤活性[J].食用菌学报,2016,23(2):70-74.[10]程继红，郑慧芬，贲伟东，等.荷叶离褶伞工厂化栽培[J].食用菌学报，2008，15(2):20-22.[11]宋莹,吕立涛,杨镇，等.烟色离褶伞液体菌种培养条件及栽培基质优化[J].食用菌学报,2015,22(4):27-30.[12]OHTA A.Production of fruit-bodies of a mycorrhizal fungus, Lyophyllum shimeji，in pure culture[J].Mycoscience,1994,35:147-151.[13]赵厚坤,梁延海.灰离褶伞人工栽培技术[J].防护林科技,2016(11):111-112.[14]汤昕明,冯云利,郭相,等.我国食药用菌栽培现状分析及展望[J].北方园艺,2019(8):148-153.[15]张文武,刘艳,邵小军,等.金针菇液体与固体菌种制作及栽培对比试验[J].中国食用菌,2012,31(5):65.[16]蒋宁,李辉平,侯立娟,等.杏鲍菇液体菌种的研究及进展[J].山西科技,2018,33（4）：50-54.[17]刘启燕,戚俊,周洪英,等.食用菌液体菌种工厂化生产应用现状及发展浅析[J].食用菌，2018,43(6):8-10.[18]江树勋,邵碧英,陈文炳,等.15种常见食（药）用菌的3种总DNA提取方法比较研究[J].食品科学,2004,25（5）:36-40.[19]WHITE T J，BRUNS T D，LEE S B，et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//Innis MA，Gelfand DH，SninskyJJeds.PCR Protocols:A Guide to Methods and Applications.New Yorks:Academic Press，1990:315-321.[20]余金凤,陈正启,周汐,等.云南省美味牛肝菌的遗传多样性分析[J].北方园艺,2018(6):139-144.[21]郭尚,徐莉娜,周林,等.Agaricus bisporus野生驯化新品种“沐野１号”的分子遗传鉴定与重金属富集规律研究[J].北方园艺,2019（8）:137-141.[22]王守现,刘宇,许峰,等.荷叶离褶伞菌株的ITS鉴定及生物学特性研究[J].中国农学通报,2012,28(28):148-152.[23]陈今朝,贺稚非,李洪军,等.野生灰离褶伞菌丝体液体发酵工艺研究[J].食品工业科技,2010,31(5):212-214.[24]席亚丽,王治江,魏生龙,等.荷叶离褶伞摇瓶发酵条件研究[J].河西学院学报,2011,27(2):68-74.[25]宋莹,吕立涛,杨镇,等.烟色离褶伞液体菌种培养条件及栽培基质优化[J].食用菌学报,2015,22(4):27-30.
相似文献/References:
[1]刘灵娣,谢晓亮,温春秀,等.不同丹参种质的rDNA ITS序列分析[J].北方园艺,2012,36(20):146.
LIU Ling-di,XIE Xiao-liang,WEN Chun-xiu,et al.rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge[J].Northern Horticulture,2012,36(02):146.
[2]赵娜娜,陈文强,邓百万,等.秦巴山区香菇主要栽培种亲缘关系的研究[J].北方园艺,2014,38(16):140.
ZHAO Na-na,CHEN Wen-qiang,DENG Bai-wan,et al.Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area[J].Northern Horticulture,2014,38(02):140.
[3]王晓峨,吴永汉.浙南山区茄子根结线虫种类鉴定[J].北方园艺,2012,36(11):137.
WANGXiao-e,WUYong-han.IdentificationoftheRoot-knotNematodeonSolanummelongenaL.in SouthernMountainAreaofZhejiang[J].Northern Horticulture,2012,36(02):137.
[4]裴冬丽,刘筱斐,陈柯.朝天委陵菜白粉病病原菌鉴定[J].北方园艺,2016,40(06):112.[doi:10.11937/bfyy.201606028]
PEI Dongli,LIU Xiaofei,CHEN Ke.Identification of Powdery Mildew of Potentilla supine[J].Northern Horticulture,2016,40(02):112.[doi:10.11937/bfyy.201606028]
[5]刘晓婷,孙国琴,李亚娇,等.一株白桩菇属野生食用菌菌种分离鉴定与培养[J].北方园艺,2017,41(18):161.[doi:10.11937/bfyy.20170461]
LIU Xiaoting,SUN Guoqin,LI Yajiao,et al.Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus[J].Northern Horticulture,2017,41(02):161.[doi:10.11937/bfyy.20170461]
[6]丁锦平,侯颖,张庆琛,等.佩兰白粉病的病原菌鉴定[J].北方园艺,2018,42(09):77.[doi:10.11937/bfyy.20174286]
DING Jinping,HOU Ying,ZHANG Qingchen,et al.Identification of Herba eupaatorii Fortunei Powdery Mildew[J].Northern Horticulture,2018,42(02):77.[doi:10.11937/bfyy.20174286]
[7]裴冬丽,朱晓琴,王书楠,等.草本药用植物猪殃殃白粉菌分子生物学鉴定[J].北方园艺,2018,42(09):149.[doi:10.11937/bfyy.20171623]
PEI Dongli,ZHU Xiaoqin,WANG Shunan,et al.Molecular Identification of Powdery Mildew of Medicinal Herb Galium aparine[J].Northern Horticulture,2018,42(02):149.[doi:10.11937/bfyy.20171623]
[8]梁姗,杨霞.基于ITS序列的胭脂萝卜亲缘关系分析[J].北方园艺,2019,43(01):30.[doi:10.11937/bfyy.20181377]
LIANG Shan,YANG Xia.Analysis of Carmine Radish Genetic Relationship Based on ITS Sequences[J].Northern Horticulture,2019,43(02):30.[doi:10.11937/bfyy.20181377]
[9]陈婉婷,倪必勇,张富强,等.基于ITS序列的栽培杏种质亲缘关系及政和杏的分类研究[J].北方园艺,2019,43(07):27.[doi:10.11937/bfyy.20182702]
CHEN Wanting,NI Biyong,ZHANG Fuqiang,et al.Genetic Relationships of Cultivation Apricot and Classification of Prunus zhengheensis Revealed by ITS Sequences[J].Northern Horticulture,2019,43(02):27.[doi:10.11937/bfyy.20182702]
[10]张家晨,贾福晨,罗章,等.基于ITS序列对林芝市售七种野生大型食用真菌的鉴定[J].北方园艺,2020,44(08):122.[doi:10.11937/bfyy.20193803]
ZHANG Jiachen,JIA Fuchen,LUO Zhang,et al.Identification Research of Seven Wild Macrofungus Based on ITS Sequences[J].Northern Horticulture,2020,44(02):122.[doi:10.11937/bfyy.20193803]
备注/Memo
第一作者简介：岳万松（1990-），男，硕士，助理研究员，研究方向为食用菌育种栽培。E-mail:459440031@qq.com.责任作者：罗孝坤（1966-），男，本科，高级农艺师，研究方向为食用菌菌种选育及栽培技术。E-mail:565043908@qq.com.基金项目：国家重点研发计划资助项目（2017YFD0601002）。收稿日期：2019-09-24