GAO Wa,ZHANG Dongmei,GAO Zhenjiang,et al.Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties[J].Northern Horticulture,2019,43(23):20-26.[doi:10.11937/bfyy.20191132]
番茄溃疡病病原的鉴定和抗性品种的筛选
- Title:
- Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties
- 文献标志码:
- A
- 摘要:
- 以番茄“CM966”病株为试验材料，采用PCR的方法和形态学鉴定法对包头市采集的疑似番茄病株进行鉴定，并构建系统发育树，以期确定其分类地位；以17个番茄品系和品种为试验材料，采用与番茄溃疡病相关的QTL位点中贡献率最高的Rcm5.1对17个番茄品系及品种进行特异性PCR扩增，同时进行田间接种试验，探究番茄溃疡病抗性种质资源。结果表明：采自4个不同番茄种植田的疑似溃疡病病株，均携带番茄溃疡病菌。经过BLAST比对，其中4个病原菌的分离物与GenBank中已报道的密执安棒形杆菌密执安亚种（Clavibacter michiganensis subsp.michiganensis,Cmm）的一致性达90%以上，其中1个病原菌分离物序列与其它菌株亲缘关系较远；对17个番茄品系及品种进行检测，均不携带QTL位点中Rcm5.1点的抗性基因，但是田间接种试验表明有3个品系‘山东荷兰’‘圣490’及‘紫晶’表现为高抗，病情指数分别为2%、4%、4%。“160”“CM966”‘T54’及“普罗旺斯”表现为高感,病情指数均大于50%。
- Abstract:
- Using tomato ‘CM966’ disease strain as test material,the suspected tomato disease strain collected by Baotou city was identified by PCR method and morphological identification method,and the phylogenetic tree was constructed to determine its taxonomic status;17 tomato lines and cultivars were tested with specific PCR amplification and using Rcm5.1 with the highest contribution rate in the QTL locus associated with tomato canker disease,and field inoculation test was conducted to explore tomato canker resistant species resources.The results showed that the suspected canker disease strains from four different tomato plantations all carried tomato canker.After BLAST comparison,the isolates of 4 pathogens were more than 90% identical to the reported Clavibacter michiganensis subsp.michiganensis (Cmm) in GenBank,including 1 pathogen.The isolate sequence was significantly different from other strains;17 tomato lines and cultivars were tested,and none of the resistance genes at the Rcm5.1 point in the QTL locus was carried.However,field vaccination trials showed that three strains ‘Shandong Holland’,‘Sheng 490’ and ‘Amethyst’ showed high resistance,with disease indices of 2%,4%,and 4%,respectively.‘160’‘CM966’‘T54’ and ‘Provence’ showed high susceptible,and the disease index was greater than 50%.
参考文献/References:
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备注/Memo
第一作者简介：高娃(1987-)，女，硕士，助理研究员，现主要从事植物保护等研究工作。E-mail:gaowabao@163.com.责任作者：张冬梅(1969-)，女，本科，研究员，现主要从事植保农化等研究工作。E-mail:zhangdongmei69@126.com.基金项目：内蒙古自治区番茄工程中心资助项目（CX2017-51）。收稿日期：2019-05-03