GAO Guo-ping,WANG Hong,DENG Qiu-yue,et al.Study on Pathogen Identification and Biological Characteristics of Pathogenic Fungal of Poplar Leaf Margin Blight[J].Northern Horticulture,2015,39(04):119-123.[doi:10.11937/bfyy.201504028]
杨树叶缘枯病病原菌鉴定及生物学特性
- Title:
- Study on Pathogen Identification and Biological Characteristics of Pathogenic Fungal of Poplar Leaf Margin Blight
- Keywords:
- Populus; identification; biological characteristics; ITS rDNA
- 文献标志码:
- A
- 摘要:
- 以从杨树发病叶分离到的病原菌为试材，采用形态学与分子生物学法鉴定病原菌，利用菌丝培养法，研究不同培养基、温度、光照、pH值、碳源和氮源对病原菌菌落生长的影响。结果表明：病原菌为大茎点属（Macrophoma sp.）真菌；病原菌菌丝生长最适温度为25～30℃，适宜pH值为6.0，适宜培养基为PA培养基与PDA培养基，不同的光照条件对病原菌菌丝生长速度影响不大，适宜病原菌P1生长的最佳碳源为乳糖，氮源为酵母膏。
- Abstract:
- Taking the leaves infected by poplar isolated pathogenic bacteria as experimental materials，pathogenic bacteria was identified by morphology and molecular biology method，the effects of different culture medium,temperature,light,pH value,carbon source and nitrogen source on colony growth were studied by method of mycelium culture.The results showed that pathogen P1 was identified as Macrophoma sp..The optimum temperature of the mycelium growth was 25-30℃,the optimum pH value was 6,PA medium and PDA medium were suitable,and different illumination had no obvious effect on mycelium growth.The mycelium grew best on media with Lactose as carbon source，yeast as nitrogen resource.
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备注/Memo
第一作者简介：高国平（1961-），男，博士，教授，研究方向为林木病理学和园林植物病理学。E-mail：ggp8881@163.com.