FENG Youren,LIU Baosheng,BAI Penghua.Pathogen Identification and Biological Characteristics of Rose Leaf Blotch in Tianjin[J].Northern Horticulture,2015,39(14):125-129.[doi:10.11937/bfyy.201514033]
天津市新型月季叶枯病病原菌鉴定及生物学特性研究
- Title:
- Pathogen Identification and Biological Characteristics of Rose Leaf Blotch in Tianjin
- Keywords:
- Rosa chinensis Jacq; leaf blotch; pathogen identification; biological characteristics; Pestalotiopsis clavispora (Arth.)Stey.
- 文献标志码:
- A
- 摘要:
- 以天津市某花圃栽培中发现的一种新型病害为研究对象，采用形态特征观察、致病性测定及rDNA-ITS测序、比对研究方法，对其进行了病原鉴定，并对病原菌的生物学特性进行了简单研究。结果表明：该菌株在PDA培养基上4 d菌落直径达到34.6～37.2 mm，具明显的灰白色轮纹。分生孢子呈梭形，宽度为4.83～9.11 μm，由5个细胞组成，细胞间有隔膜，孢子顶端有2～3根附属丝，长度约为5.93～36.23 μm，尾胞具附属丝1根，长度为2.85～16.05 μm。其rDNA-ITS测序比对结果表明，该菌株与棒状拟盘多毛孢菌Pestalotiopsis clavispora的同源性达到99%。基于形态学特征和rDNA-ITS测序比对结果，该病原菌被最终鉴定为Pestalotiopsis clavispora (Arth.)Stey.，经检索月季病害,属国内首次发现由棒状拟盘多毛孢菌引起月季叶枯病。生物学特性研究表明，该菌生长最适温度是25℃，但20～30℃范围内差异不显著；适宜pH 5.0；最佳碳源为乳糖。
- Abstract:
- Taking one disease on leaf of Rosa chinersis that found in Tianjin flower nursery as test material，the pathogen was identified through morphological characteristics，pathogenicity and rDNA-ITS sequence analysis，simple biological characteristics research of this pathogen was conducted also.The results showed that fungal colony that developed on PDA was 34.6-37.2 mm in diameter，with concentric rings in white in four days.The conidia was fusiform and 4.83-9.11 μm in diameter，formed by five cells with four transverses；the apical appendage of conidia was 2-3，5.93-36.23 μm in length；the basal appendage of conidia was 1，2.85-16.05 μm in length.The homology of rDNA-ITS sequence between this pathogen and Pestalotiopsis clavispora was 99%.Based on the morphological characteristics and rDNA-ITS sequence analysis，the pathogen was finally identified as P.clavispora.This was the first report that Pestalotiopsis clavispora could cause leaf blotch disease of Rosa chinensis Jacq in China.Biological characteristics studies demonstrated that the optimum temperature for growth was 25℃，no significant difference existed in the range of 20-30℃；the suitable pH value was around 5.0；the suitable carbon source was lactose.
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备注/Memo
第一作者简介：冯友仁（1983-），男，硕士，研究实习员，研究方向为植物保护和病虫害生物防治。E-mail：znlfyr@126.com.