[1]孙蕾,白雪,白庆荣,等.甜瓜青霉果腐病的病原鉴定及药剂筛选[J].北方园艺,2019,43(18):52-57.[doi:10.11937/bfyy.20190305]
　SUN Lei,BAI Xue,BAI Qingrong,et al.Pathogen Identification and Screening of the Fungicides of Muskmelon Fruit Rot Caused by Penicillium oxalicum[J].Northern Horticulture,2019,43(18):52-57.[doi:10.11937/bfyy.20190305]

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甜瓜青霉果腐病的病原鉴定及药剂筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年18 页码: 52-57 栏目: 出版日期: 2019-09-30

Title:: Pathogen Identification and Screening of the Fungicides of Muskmelon Fruit Rot Caused by Penicillium oxalicum

作者:: 孙蕾1; 白雪2; 白庆荣1; 赵廷昌2; （1.吉林农业大学植物保护学院，吉林长春 130118；2.中国农业科学院植物保护研究所，北京 100193）

Author(s):: SUN Lei1; BAI Xue2; BAI Qingrong1; ZHAO Tingchang2; (1.Institute of Plant Protection，Jilin Agricultural University,Changchun,Jilin 130118；2.Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193)

关键词:: 甜瓜; 草酸青霉; 果腐病; 病原鉴定; 药剂筛选

Keywords:: muskmelon; Penicillium oxalicum; fruit rot; pathogen identification; screening of the fungicides

DOI:: 10.11937/bfyy.20190305

文献标志码:: A

摘要:: 以发病甜瓜为试材，采用常规组织分离法对采自吉林省梨树县甜瓜上的一种新病害进行病原菌分离、致病性测定，并通过形态学特征及rDNA-ITS、NS、NL、CAM和RPB2序列分析，确定其为草酸青霉菌（Penicillium oxalicum）。采用孢子萌发法测定了该病原菌对20种杀菌剂的敏感性，以期筛选出对防治甜瓜青霉果腐病有效的药剂。结果表明：草酸青霉对25%吡唑醚菌酯EC、17%唑醚?氟环唑SC、80%克菌丹WG、70%代森联WG、22.5%啶氧菌酯SC、75%肟菌?戊唑醇WG和60%唑醚?代森联WG敏感性较高，EC50为0.001 7~1.000 0 mg?L-1；对75%百菌清WP、450 g?L-1咪鲜胺EW、500 g?L-1甲基硫菌灵、25%嘧菌酯SC、250 g?L-1苯醚甲环唑EC和12%氟菌?氟环唑EC的敏感性略低，EC50为1.379 5~8.131 5 mg?L-1，为生产上防治该病害提供了备选药剂。

Abstract:: Pathogens which caused muskmelon fruit rot were gotten by tissue isolation method.Their pathogenicity were confirmed according to the Koch′s Postulation，and all of them were the pathogens.The pathogens were identified as Penicillim oxalicum based on morphological characteristics and rDNA-ITS，NS，NL，CAM and RPB2 sequence analysis.The sensitivity of the representative strain TGQM01 pathogens to 20 fungicides was tested to determine effective treatments for controling muskmelon fruit rot by spore germination method.The results showed that the spores of P.oxalicum was more sensitive to 25% pyraclostrobin EC，17% yraclostrobin?epoxiconazole SC，80% captan WG，70% polyram WG，22.5% picoxystrobin SC，75% trifloxystrobin?tebuconazole WG and 60% pyraclostrobin?metiram WG (0.001 7 mg?L-1EC501.000 0 mg?L-1).Followed by 75% chlorothalonil WP，450 g?L-1 prochloraz EW，500 g?L-1 thiophanatemethyl，25% azoxystrobin SC，250 g?L-1 difenoconazole EC and 12% fluxapyroxad?epoxiconazole EC (1.379 5 mg?L-1EC508.131 5 mg?L-1).These results provided theoretical basis for controlling of the disease.

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备注/Memo

第一作者简介：孙蕾（1992-），女，硕士研究生，研究方向为植物病害综合治理。E-mail:272784398@qq.com.责任作者：白庆荣（1975-），女，博士，教授，硕士生导师，现主要从事植物病害综合治理等研究工作。E-mail:bbbqqqrrr@163.com.基金项目：国家重点研发计划资助项目（2018YFD0201300）；国家西甜瓜产业技术体系资助项目(CARS-25)。收稿日期：2019-03-21

更新日期/Last Update: 2019-09-29