[1]张萍,姜景彬,杨欢欢,等.番茄EMS突变体库的构建及表型分析[J].北方园艺,2019,43(07):1-9.[doi:10.11937/bfyy.20183698]
　ZHANG Ping,JIANG Jingbin,YANG Huanhuan,et al.Creation and Phenotypic Analysis of Tomato EMS Mutant Library[J].Northern Horticulture,2019,43(07):1-9.[doi:10.11937/bfyy.20183698]
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番茄EMS突变体库的构建及表型分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年07 页码: 1-9 栏目: 出版日期: 2019-04-15
Title:
Creation and Phenotypic Analysis of Tomato EMS Mutant Library
作者:
张萍; 姜景彬; 杨欢欢; 赵婷婷; 许向阳; 李景富
（东北农业大学 园艺园林学院，黑龙江 哈尔滨　150030）
Author(s):
ZHANG Ping; JIANG Jingbin; YANG Huanhuan; ZHAO Tingting; XU Xiangyang; LI Jingfu
(School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
关键词:
番茄; 甲基磺酸乙酯诱变; 突变体库; SSR
Keywords:
tomato; ethyl methyl sulfonate mutagenesis; mutant library; SSR
DOI:
10.11937/bfyy.20183698
文献标志码:
A
摘要:
以番茄品种“14637” 种子为试材，采用0.8%的甲基磺酸乙酯（EMS）处理种子12 h，然后分别在叶、花、茎、果实、种子等方面对M1和M2世代群体单株进行表型变异观察，同时利用20对SSR引物对M2代典型变异株系进行分析鉴定，构建番茄突变体库，以期为获得番茄新种质资源提供参考依据。结果表明：M1代群体发生了一些变异，如植株矮化、叶色变异、花朵畸形、茎细弱、果实畸形等，但表型变异现象不明显；M2代番茄群落筛选共发现65种变异类型，189株变异植株，突变率为12.89%，其中获得的有益突变类型有8种；对M3代中13个典型变异植株进行基因组水平的变异检测，通过SSR引物分析，发现13种突变体均发生了DNA水平上的变异。该研究初步构建了含有189株M2代植株、65种表型变异的番茄突变体库。
Abstract:
The seeds of tomato variety ‘14637’ were used as materials,the seeds were treated with 0.8% methyl sulfonate (EMS) for 12 hours,and then the phenotypic variation was observed in the leaf,flower,stem,fruit,seed and the like in the group of M1 and M2 population groups,and the analysis and evaluation of the typical mutant strain of the M2 typical mutation were analyzed by 20 pairs of SSR primers,and the tomato mutant library was constructed in order to provide reference for obtaining new tomato germplasm resources.The results showed that the M1-generation population had some variation,such as plants dwarfing,leaf color variation,flower malformation,stem thinness and fruit malformation,but the phenotypic variation was not obvious.A total of 65 variation types were found in the selection of tomato communities of M2 generation,and 189 mutant plants were found with a mutation rate of 12.89%,among which 8 beneficial mutation types were obtained.The variation of the genome level of 13 typical mutant plants in the M3 generation was tested.By SSR primer analysis,it was found that all 13 mutants had variation at the DNA level.This study preliminarily constructed a library of tomato mutant containing 189 strain M2 plants and 65 phenotypic variation.

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备注/Memo

第一作者简介：张萍（1994-），女，硕士研究生，研究方向为番茄育种。E-mail：920690217@qq.com.责任作者：李景富（1943-），男，教授，博士生导师，研究方向为番茄育种。E-mail：Lijf_2005@126.com.基金项目：国家自然科学基金面上资助项目（31572137）；“十三五”国家重点研发计划资助项目（2016YFD0101703）；国家自然科学基金资助项目（31501777）；哈尔滨市应用技术研究与开发资助项目（2017RAQXJ005）。收稿日期：2018-12-20

更新日期/Last Update: 2019-04-30